Influence of allicin administration on reproductive efficiency, immunity and lipid peroxidation of rabbit does under high ambient temperature

Abstract

This study investigated the effect of daily oral administration with allicin levels (0, 5 and 10 mg/kg of female body weight), 30 days pre‐insemination, on reproductive performance in vivo and in vitro, immunity, and oxidative stress of rabbit does under high ambient temperature. Niliparous NZW does (n = 105) were randomly divided into three groups (35 in each) treated with 0, 5 and 10 mg allicin dissolved in 2 ml distilled water, respectively, for 30 days pre‐insemination. At the end of treatment (30 days), does were artificially inseminated with fresh diluted semen of 20 fertile NZW bucks. Reproductive performance and ovulatory response parameters were determined. Serum biochemicals, enzyme activity, immunoglobulins (IgG and IgM) and antioxidant status were determined on day 30 of treatment. Serum progesterone and prolactin were determined pre‐insemination (30 days of treatment), on 15 days of pregnancy and 7 days post‐partum. Results showed that both allicin levels increased live litter size, and bunny viability rat and litter size at birth and weaning. Allicin levels increased ovulation rate and improved embryo quality. Number of total follicles decreased only with 10 mg allicin. Progesterone increased pre‐insemination, 15 days of pregnancy and 7 days post‐partum progesterone by allicin levels. Prolactin pre‐insemination and on day 7 post‐partum increased with 10 mg allicin. Serum total proteins, albumin, globulin, IgG and IgM increased, while glucose, aspartate and alanine aminotransaminases, and thiobarbituric acid reaction decreased by both allicin levels. In conclusion, the mechanism by which allicin administration 30 days pre‐insemination to improve the reproductive performance of rabbit does is based on that allicin can play an important role, as a natural exogenous antioxidant, increasing immune response and reducing lipid peroxidation.

Effects of dietary phytogenic supplementation on performance, semen quality and serum biochemical of rabbits

“…In the current study, supplementation of TEO to male rabbits signi cantly decreased serum urea, creatinine, ALT and AST activity, as well as increased serum testosterone levels compared to PC and NC groups. These results are in accordance with those of El-Ratel et al [ 13] reported that the activity of AST and ALT of rabbits were signi cantly improved by oral administration of phytogenic (5 or 10 mg allicin/ BW) compared with the control. Supplementation of 2.5% of thyme leaves to rabbit diets signi cantly decreased urea and creatinine [ 14].…”

“…El-Ratel et al [ 13] reported that oral administration of phytobiotic improved liver function of rabbits compared with the control. Likewise, supplementation of 2.5% of thyme leaves to rabbit diets signi cantly improved kidney function by decreased urea and creatinine levels [ 14].…”

Effects of dietary phytogenic supplementation on performance, semen quality and serum biochemical of rabbits

“…In the current study, supplementation of TEO to male rabbits significantly reduced serum urea, creatinine, and ALT and AST activities and increased serum testosterone levels compared to PC and NC groups, but the values were within the normal physiological range [14,16,[29][30][31]. These results agree with those of El-Ratel et al [14], where it is reported that the activity of AST and ALT of rabbits was remarkably enhanced by oral administration of phytogenic additives (5 or 10 mg allicin/BW) compared to the control. Supplementation of 2.5% of thyme leaves to rabbit diets significantly decreased urea and creatinine [15].…”

Productive and Physiological Response of Male Rabbits to Dietary Supplementation with Thyme Essential Oil

“…In the current study, supplementation of TEO to male rabbits significantly reduced serum urea, creatinine, and ALT and AST activities and increased serum testosterone levels compared to PC and NC groups, but the values were within the normal physiological range [ 14 , 16 , 29 , 30 , 31 ]. These results agree with those of El-Ratel et al [ 14 ], where it is reported that the activity of AST and ALT of rabbits was remarkably enhanced by oral administration of phytogenic additives (5 or 10 mg allicin/BW) compared to the control. Supplementation of 2.5% of thyme leaves to rabbit diets significantly decreased urea and creatinine [ 15 ].…”

“…These active components had the best oxidative status [ 12 ] and may reduce serum cholesterol [ 13 ], which could improve the reproductive performance of rabbits. El-Ratel et al [ 14 ] reported that oral administration of phytobiotics improved the liver function of rabbits compared to the control. Similarly, supplementation of 2.5% of thyme leaves to rabbit diets remarkably enhanced kidney function by decreasing urea and creatinine levels [ 15 ].…”