Influence of alcoholic modifiers on the selectivity of the separation of a group of polycyclic aromatic hydrocarbons by micellar liquid chromatography

Delgado, Miguel Ángel Rodríguez; Sánchez, M. J.; González, Venerando; Montelongo, F. García

doi:10.1016/0003-2670(94)00287-8

Cited by 27 publications

(6 citation statements)

References 19 publications

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“…Khaledi et al 47 have found that for 14 aromatic compounds belonging to different classes the values of S hyb in the cetyltrimethylammonium bromide micellar phase, modified by methanol, 2-propanol and n-butanol, vary in the order S n-butanol > S 2-propanol > S methanol , which coincides with the increase in the eluting strength of these modifiers in RP HPLC. An analogous sequence was obtained by Rodriguez Delgado et al 65 for 15 PAH in the mobile micellar phase of sodium dodecyl sulfate modified with the same alcohols. 65 The higher values of the parameter S hyb for 2-butanol indicate that it interacts with the micelles more actively, solvates the sorbates more effectively and can compete better with the micelles for the interaction with sorbates than does methanol.…”

Section: Effect Of the Nature Of The Organic Modifiersupporting

confidence: 53%

“…An analogous sequence was obtained by Rodriguez Delgado et al 65 for 15 PAH in the mobile micellar phase of sodium dodecyl sulfate modified with the same alcohols. 65 The higher values of the parameter S hyb for 2-butanol indicate that it interacts with the micelles more actively, solvates the sorbates more effectively and can compete better with the micelles for the interaction with sorbates than does methanol.…”

Section: Effect Of the Nature Of The Organic Modifiersupporting

confidence: 53%

“…67 The selectivities of separation attainable with 2-propanol, acetonitrile and tetrahydrofuran belonging to different types of solvents are close, 67 whereas those with 2-propanol and n-butanol belonging to the same type exhibited strong differences. 9,65,67 For example, in a model mixture of 15 polyaromatic hydrocarbons (PAH) in the absence of the modifier (0.15 M sodium dodecyl sulfate) in the mobile phase, many compounds are co-eluted, particularly the most hydrophobic ones. 65 In the presence of 15 vol.% methanol, the peaks of all compounds are well resolved.…”

Section: Effect Of the Nature Of The Organic Modifiermentioning

confidence: 99%

“…9,65,67 For example, in a model mixture of 15 polyaromatic hydrocarbons (PAH) in the absence of the modifier (0.15 M sodium dodecyl sulfate) in the mobile phase, many compounds are co-eluted, particularly the most hydrophobic ones. 65 In the presence of 15 vol.% methanol, the peaks of all compounds are well resolved. The quality of separation with 2-propanol instead of methanol is similar; in this case, the time of analysis is reduced from 45 to 25 min.…”

Section: Effect Of the Nature Of The Organic Modifiermentioning

confidence: 99%

“…Variation of S hyb as a function of surfactant concentration is also different for different modifiers: as the concentration of sodium dodecyl sulfate in the mobile phase increases from 0.06 to 0.14 M, the DS m varies by *0.2 for methanol, *0.4 for 2-propanol and *2.0 for n-butanol. 65 Less hydrophobic sorbates interact strongly with the micelles (e.g., anthracene with cetyltrimethylammonium bromide 47 or naphthalene, acenaphthylene, fluorene and anthracene with sodium dodecyl sulfate 65 ). They are less accessible to such polar solvents as methanol.…”

Section: Effect Of the Nature Of The Organic Modifiermentioning

confidence: 99%

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Micellar liquid chromatography

Basova¹,

Иванов²,

Шпигун³

1999

Russ. Chem. Rev.

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Section: Effect Of the Nature Of The Organic Modifiersupporting

confidence: 53%

Section: Effect Of the Nature Of The Organic Modifiersupporting

confidence: 53%

Section: Effect Of the Nature Of The Organic Modifiermentioning

confidence: 99%

Section: Effect Of the Nature Of The Organic Modifiermentioning

confidence: 99%

Section: Effect Of the Nature Of The Organic Modifiermentioning

confidence: 99%

See 3 more Smart Citations

Micellar liquid chromatography

Basova¹,

Иванов²,

Шпигун³

1999

Russ. Chem. Rev.

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Separation and on‐column preconcentration of some nonsteroidal anti‐inflammatory drugs by microemulsion electrokinetic capillary chromatography using high‐speed separations

et al. 2005

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Various strategies have been investigated for separating a group of nonsteroidal anti-inflammatory drugs (NSAIDs) by microemulsion electrokinetic capillary chromatography (MEEKC) using high-speed separations. The parameters that of affect the separation, such as the nature of the oil droplet and the buffer, and the surfactant concentration have been studied. In addition, several organic solvents were used to decrease the retention of the analytes in the oil droplet phase and to improve the resolution of the NSAIDs. The optimum microemulsion background electrolyte (BGE) solution made of 0.8% w/w ethyl acetate, 6.6% w/w butan-1-ol, 6.0% w/w acetonitrile, 1.0% w/w sodium dodecyl sulfate (SDS), and 85.6% w/w of 10 mM sodium tetraborate at pH 9.2 resolved the drugs within 8 min. The short-end injection procedure is an alternative for reducing the analysis time. When this procedure was used, the microemulsion BGE solution consisted of 0.8% w/w ethyl acetate, 6.6% w/w butan-1-ol, 17.0% w/w methanol, 1.0% w/w SDS, and 74.6% w/w of 10 mM sodium tetraborate, pH 9.2, and the NSAIDs were separated within 3 min. The reversed electrode polarity stacking mode (REPSM) technique was applied to the on-line concentration of the NSAIDs. In this technique, the sample matrix was pumped out of the capillary using a polarity-switching step. When this technique was applied, the sensitivity was enhanced up to 40-fold and the limits of detection (LODs) were in the low microg.L(-1) levels.

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Enantioseparation of chiral tropa alkaloids by means of cyclodextrin‐modified microemulsion electrokinetic chromatography

Bitar

Holzgrabe

2007

Electrophoresis

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CD-modified microemulsion EKC as a CE technique has been applied to the chiral separation of atropine, scopolamine, ipratropium and homatropine. Enantioseparations of these tropa alkaloids were optimized by using a standard oil-in-water (O/W) microemulsion and varying the nature and concentration of CD additives as well as of the organic modifier (methanol, 2-propanol or ACN) whilst keeping the applied voltage of 15 kV and capillary temperature of 30 degrees C constant. The standard (O/W) microemulsion BGE solution consisted of 0.8% w/w octane, 6.6% w/w 1-butanol, 2.0% w/w SDS and 90.6% w/w 10 mM sodium tetraborate buffer (pH 9.2). Enantioseparations with high resolution and short migration times of all tropa alkaloids were achieved by using heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-CD and sulfated beta-CD in the microemulsion BGE and were superior to corresponding CD-modified CE methods.

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Influence of alcoholic modifiers on the selectivity of the separation of a group of polycyclic aromatic hydrocarbons by micellar liquid chromatography

Cited by 27 publications

References 19 publications

Micellar liquid chromatography

Micellar liquid chromatography

Separation and on‐column preconcentration of some nonsteroidal anti‐inflammatory drugs by microemulsion electrokinetic capillary chromatography using high‐speed separations

Enantioseparation of chiral tropa alkaloids by means of cyclodextrin‐modified microemulsion electrokinetic chromatography

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