KeyWordsColumn liquid chromatography Micella r chromatog ra phy SodTum dodecyl sulfate Cytostatic antibiotics of rubomicin derivatives Summary Micellar ITquTd chromatography has been adopted for separation of cytostatic antibiotics of rubomidn derivatives. The influence of the kind of organic modifier (normal and iso-alcehols) and its cencentratTon, sodTum dodecyl sulfate micelle concentratTon and column temperature on the chromatographic characteristics of rubomicn derivatives have been investigated. ]_ pentanol and iso propanol were shown to be the most suitable for providing optimum selectiv ity, good taTITng factor and column efriciencywth mTnimum reagent consumption.reversed-phase chromatography. MLC provides additional possibilities to control the selectivity and retention by variation of micelle concentration, as well as organic modifier and its concentration in the mobile phase.The objective in this work is to optimize chromatographic conditions for separation and determination of rubomicin derivatives using the micellar mobile phase with sodium dodecyl sulfate (SDS). For this purpose, the influence of the surfactant concentration, pH, ionic strength, column temperature, organic modifier and its concentration on chromatographic characteristics of rubomicin derivatives were investigated.