Influence of age, sex, lactational state and exogenous growth hormone on erythrocyte UMP synthase in dairy cattle

Jones, L. R.; Harden, Kathryn K.; Bragg, Dawn; Robinson, James L.

doi:10.1016/0305-0491(86)90112-4

Cited by 5 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The assay of UMP synthase employed has been described by L. R. Jones et al (1986) and is a modification of that of M. E. Jones et al (1978). It involves measuring the decarboxylation of [7-14C]orotic acid (NEN, Boston, MA; 41.25 mCi mmol-1) which occurs during its conversion to UMP.…”

Section: Ump Synthasementioning

confidence: 99%

“…Each pair consisted of an animal heterozygous for UMP synthase and a control matched for age, sex, and stage of lactation; the first two factors have been shown to contribute to variation in UMP synthase activity (Jones et al, 1986). Samples from members of each pair were obtained, prepared, and assayed concurrently.…”

Section: Statistical Proceduresmentioning

confidence: 99%

“…To measure the red blood cell transferase alone, excess yeast OMP decarboxylase (ODC; Sigma, St. Louis, MO) was added in the UMP synthase assay (Jones et al, 1986) to ensure that the decarboxylase was not the limiting reaction. To each vial, 0.25 units ODC (1 unit converts 1/~mol OMP to UMP per hour at 37°C) was added.…”

Section: Orotate Phosphoribosyltransferase (Oprt)mentioning

confidence: 99%

“…Blood samples were obtained and haemolysates prepared as described by Jones et al (1986) with the following modifications; haemolysates were stored at -70°C, haemolysates assayed for PRPP concentration and PRPP synthetase activity were prepared by diluting red blood cells with an equal volume of cold double-distilled water containing 5 mmolL -1 EDTA, pH7.0, and assays were conducted within 2 weeks of blood collection except those for PRPP concentration which were performed within 2 days.…”

Section: Blood and Tissue Collectionmentioning

confidence: 99%

“…Longevity is not compromised and growth is normal in cattle heterozygous for UMP synthase deficiency (Jones et at., 1986;Shanks et al, 1987a). However, heterozygous cows have a longer calving interval than normal (Shanks et al, 1986b) and some have proved difficult to impregnate.…”

mentioning

confidence: 98%

See 4 more Smart Citations

Deficiency of UMP synthase in dairy cattle: A model for hereditary orotic aciduria

Harden

Robinson

1986

J of Inher Metab Disea

View full text Add to dashboard Cite

Deficiency of uridine-5'-monophosphate (UMP) synthase in dairy cattle, a condition analogous to human hereditary orotic aciduria, is reviewed with consideration of similarities and differences between the enzyme deficiency in humans and cattle. New findings regarding the bovine condition are reported including presence of the enzyme deficiency in numerous tissues and absence of substantial effects on other aspects of nucleotide metabolism. Specifically, erythrocyte concentration of phosphoribosylpyrophosphate (PRPP) and activities of PRPP synthetase, adenine phosphoribosyltransferase, and hypoxanthine-guanine phosphoribosyltransferase appear to be normal in cattle heterozygous for UMP synthase deficiency.

show abstract

Section: Ump Synthasementioning

confidence: 99%

Section: Statistical Proceduresmentioning

confidence: 99%

Section: Orotate Phosphoribosyltransferase (Oprt)mentioning

confidence: 99%

Section: Blood and Tissue Collectionmentioning

confidence: 99%

mentioning

confidence: 98%

See 3 more Smart Citations

Deficiency of UMP synthase in dairy cattle: A model for hereditary orotic aciduria

Harden

Robinson

1986

J of Inher Metab Disea

View full text Add to dashboard Cite

show abstract

Characterization of UMP synthase in dairy cattle heterozygous for a lethal recessive trait

Harden

Robinson

1987

Biochem Genet

View full text Add to dashboard Cite

UMP synthase was characterized biochemically in dairy cattle heterozygous for a deficiency of this enzyme. Both activities comprising this bifunctional enzyme are decreased, with OMP decarboxylase more affected than orotate phosphoribosyltransferase. Immunotitration of UMP synthase activity revealed the presence of the protein product of the mutant allele in the heterozygous animals. UMP synthases from normal and deficient cattle were not distinguished from one another by kinetic constants, responses to inhibitors, pH profiles, or thermal lability. It was concluded that the 50% reduction in enzyme activity in heterozygous cattle is the result of the presence of only half the normal level of catalytically active UMP synthase.

show abstract

Identification of the homozygous recessive genotype for the deficiency of uridine monophosphate synthase in 35-day bovine embryos

Shanks¹,

Popp²,

McCoy³

et al. 1992

Reproduction

View full text Add to dashboard Cite

Holstein-Friesian cattle heterozygous for the deficiency of uridine monophosphate (UMP) synthase have half-normal activity of UMP synthase. The homozygous recessive genotype would result in little or no activity, has not been observed among live animals and apparently leads to embryonic mortality at approximately Day 40 of gestation. Activity of UMP synthase averaged 2.74 +/- 0.61 units/mg protein for 19 obligatory normal embryos (from normal x normal matings). Activity for 18 embryos from heterozygote x heterozygote matings yielded three non-overlapping groups as follows: (i) five presumed normals with greater than two-thirds normal activity, (ii) ten apparent heterozygotes with one-third to two-thirds normal activity and (iii) three putative homozygous recessive embryos with less than one-third normal activity. The distribution among these groups was consistent with the 1:2:1 ratio expected for autosomal inheritance. Conception of embryos homozygous recessive for this disorder was demonstrated.

show abstract

Influence of age, sex, lactational state and exogenous growth hormone on erythrocyte UMP synthase in dairy cattle

Cited by 5 publications

References 8 publications

Deficiency of UMP synthase in dairy cattle: A model for hereditary orotic aciduria

Deficiency of UMP synthase in dairy cattle: A model for hereditary orotic aciduria

Characterization of UMP synthase in dairy cattle heterozygous for a lethal recessive trait

Identification of the homozygous recessive genotype for the deficiency of uridine monophosphate synthase in 35-day bovine embryos

Contact Info

Product

Resources

About