Influence of Age on Calvarial Critical Size Defect Dimensions: A Radiographic and Histological Study

Hudieb, Malik; Haddad, Adeeb; Bakeer, Mohammad; Alkhazaaleh, Ahmad; Alkhader, Mustafa; Taani, D. Quteish; Kasugai, Shohei

doi:10.1097/scs.0000000000007690

Cited by 14 publications

(7 citation statements)

References 38 publications

(90 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The absence of total repair in the defects of the sham group (without ovariectomy and receiving water) in the evaluated period led us to conclude that 5mm-diameter defects are of critical size since the definition of critical size defect depends on the association of the time and repair tissue analysis. Besides, the literature shows that the diameter of 5 mm is suitable to be called a critical size defect, as reported by Hudieb et al 22 . The morphological evaluation performed in the present investigation showed a positive effect of lycopene in the bone repair of Ovx rats, promoting a new bone with an abundance of osteocytes compared to the other groups.…”

Section: Discussionmentioning

confidence: 86%

Lycopene enhances bone neoformation in calvaria bone defects of ovariectomized rats

et al. 2023

View full text Add to dashboard Cite

Osteoporosis can affect a significant part of the population and fractures are the most common complications associated with this disease, leading to high public health costs. Thus, the prevention of fractures is relevant to individuals with signs and symptoms as well as to the health system. Postmenopausal osteoporosis has been associated with oxidative stress, emphasizing the importance of an efficient defense system to maintain bone health. Lycopene is a carotenoid with antioxidant properties that may stimulate osteoblastogenesis and inhibit osteoclastogenesis. The purpose of this investigation was to analyze the influence of lycopene in the bone neoformation of calvaria defects in ovariectomized rats utilizing the concentration of 45 mg/kg. Wistar Hannover female rats were divided into ovariectomized and sham groups. The ovariectomized animals received 45 mg/kg lycopene (OvxL) or water (Ovx) by daily gavage the day after ovariectomy/sham surgery for 16 weeks. Twelve weeks after ovariectomy, there were performed 5-mm calvaria defects followed by euthanasia after 4 weeks. Samples of bone tissue were collected to perform morphological and morphometrical analysis of the neoformed bone area, and percentage with Software Image J. Morphological evaluation showed mature bone with more osteocytes in the group OVxL when compared to the other groups. The morphometrical analysis demonstrated a significant increase of bone neoformation in the group OvxL (p<0.05). The data obtained suggest that lycopene benefits bone repair in the absence of estrogenic hormones.

show abstract

Section: Discussionmentioning

confidence: 86%

Lycopene enhances bone neoformation in calvaria bone defects of ovariectomized rats

et al. 2023

View full text Add to dashboard Cite

show abstract

“…For the first study, a critical size defect in the calvaria was employed, which is a standard model for testing new materials. We chose the standard size for critical size defects in calvarial rat models of 5 mm[ 45 ]. BMP-7 was used as a positive control as its effects on bone formation have been described before.…”

Section: Resultsmentioning

confidence: 99%

Bone Sialoprotein Immobilized in Collagen Type I Enhances Bone Regeneration In vitro and In vivo

Kriegel¹,

Schlösser²,

Habeck³

et al. 2022

IJB

View full text Add to dashboard Cite

The use of bioactive molecules is a promising approach to enhance the bone healing properties of biomaterials. The aim of this study was to define the role of bone sialoprotein (BSP) immobilized in collagen type I in various settings. In vitro studies with human primary osteoblasts in mono- or in co-culture with endothelial cells demonstrated a slightly increased gene expression of osteogenic markers as well as an increased proliferation rate in osteoblasts after application of BSP immobilized in collagen type I. Two critical size bone defect models were used to analyze bone regeneration. BSP incorporated in collagen type I increased bone regeneration only marginally at one concentration in a calvarial defect model. To induce the mechanical stability, three-dimensional printing was used to produce a stable porous cylinder of polylactide. The cylinder was filled with collagen type I and immobilized BSP and implanted into a femoral defect of critical size in rats. This hybrid material was able to significantly induce bone regeneration. Our study clearly shows the osteogenic effect of BSP when combined with collagen type I as carrier and thereby offers various approaches and options for its use as bioactive molecule in bone substitute materials.

show abstract

“…The rat calvarial CSD has been utilized extensively in the exploration and establishment of a wide range of regenerative materials and procedures, 32 and it has been regarded as one of the most reliable in‐vivo models in the field of bone regeneration 7,8 . The 5 mm diameter of the CSD used in the present study is considered as an adequate size for evaluating new bone formation 33,34 .…”

Section: Discussionmentioning

confidence: 99%

The impact of Resolvin E1 on bone regeneration in critical‐sized calvarial defects of rat model—A gene expression and micro‐CT analysis

Alrumaih,

Alshibani,

Alssum

et al. 2023

J of Periodontal Research

View full text Add to dashboard Cite

ObjectiveTo investigate, in vivo, the effect of local application of Resolvin E1 (RvE1) on the bone regeneration of critical‐size defects (CSDs) in Wistar rats utilizing gene expression and micro‐computed tomographic (micro‐CT) analysis.BackgroundThe inflammation‐resolving actions of RvE1 are well established. The molecular mechanism of its bone‐regenerative actions has been of significant interest in recent years; however, there is limited information regarding the same.Materials and MethodsThirty Wistar rats with a 5 mm induced critical‐size calvarial defect were randomly allocated into four groups: no treatment/negative control (n = 5), treatment using bovine bone grafts/positive control (n = 5), treatment using local delivery of RvE1 (n = 11) and treatment using RvE1 mixed with bovine bone graft (n = 9). After 4 weeks, RNA isolation, complementary DNA synthesis and real‐time polymerase chain reaction were used for genetic expression of alkaline phosphatase (ALP), osteocalcin (OCN) and osteopontin (OPN). The rats were sacrificed after 12 weeks and micro‐CT imaging was performed to analyse the characteristics of the newly formed bone (NFB). The data were analysed using ANOVA and the least significant difference tests (α ≤ .05).ResultsThe RvE1 + bovine graft group had statistically highest mean NFB (20.75 ± 2.67 mm3) compared to other groups (p < .001). Similarly, RvE1 + bovine graft group also demonstrated statistically highest mean genetic expression of ALP (31.71 ± 2.97; p = .008) and OPN (34.78 ± 3.62; p < .001) compared to negative control and RvE1 groups.ConclusionResolvin E1 with adjunct bovine bone graft demonstrated an enhanced bone regeneration compared to RvE1 or bovine graft alone in the calvarial defect of Wistar rats.

show abstract

Influence of Age on Calvarial Critical Size Defect Dimensions: A Radiographic and Histological Study

Cited by 14 publications

References 38 publications

Lycopene enhances bone neoformation in calvaria bone defects of ovariectomized rats

Lycopene enhances bone neoformation in calvaria bone defects of ovariectomized rats

Bone Sialoprotein Immobilized in Collagen Type I Enhances Bone Regeneration In vitro and In vivo

The impact of Resolvin E1 on bone regeneration in critical‐sized calvarial defects of rat model—A gene expression and micro‐CT analysis

Contact Info

Product

Resources

About