Influence of age, gender characteristics, chronotype on the expression of core clock genes Per1, Clock, Bmal1 and Cry1 in buccal epithelium

Abstract

The purpose of the study is to determine the expression of the core clock genes in buccal epithelial cells of healthy people with different chronotypes. Materials and methods. Fourteen healthy volunteers with a healthy periodontium and oral mucosa (7 women and 7 men) were selected for participation in the trial. The buccal epithelium sampling was performed at 07:00 am and 07:00 pm in one day by cytological brush. The surveyed patients were examined chronotypically using the Horn-Ostberg test. The determination of the mRNA expression of the Per1, Clock, Bmal1, Cry1 genes was performed by quantitative real-time PCR. Statistical analysis was performed using two-way analysis of variance followed by Bonferroni post hoc tests. Results. Per1 expression was higher in the morning, regardless of chronotype, age, and gender. The expression of the Clock demonstrated the prevalence of the evening in both chronotypes, in both men and women. Bmal1 was better expressed in the evening, regardless of age, gender, and chronotype. The expression of Cry1 did not show statistically significant differences between the indicators. Conclusions. The evening expression of Clock was higher in people with the evening chronotype than in people with the morning chronotype. The chronotype did not show any effect on the expression of Per1, Bmal1, and Cry1. Age and sex did not show any effect on the expression of the core clock genes.

Expression of periferal core molecular clock genes in oral mucosa depends on the chronotype in patients with maxillofacial cellulitis

“…Previously, when studying the expression of circadian genes over time between groups of chronotypes, regardless of gender and age of participants, it was demonstrated that the extreme evening chronotypes have a delayed phase of fluctuations in the per3 and nr1d2 circadian clock genes compared to the extreme morning types. 19 , 20 …”

Study of the Circadian Molecular Clock in Paediatric Patients With Seasonal Allergic Rhinitis: Potential Approaches to Treatment

“…Отримані в групі контролю результати дослідження, знаходять підтвердження в інших подібних роботах. Так, нещодавно, вітчизняними науковцями було продемонстровано, що в здорових людей експресія мРНК per 1 у букальному епітелії була вищою вранці, в той час як bmal1 була більш вираженою у вечірній час незалежно від хронотипу, віку та статі [26]. Аналіз додаткових зарубіжних робіт, наводить дані, що у здорових людей експресія мРНК per1 в слизовій оболонці ротової порожнини та шкіри має найвищі показники в першій половині дня, з наступним зниженням впродовж доби (Bjarnason G. A., 2001).…”