Influence of age and season on certain biochemical constituents of seminal plasma of Arabian horses

Abou-Ahmed, M.M.; El-Belely, M.S.; Ismail, Somaia; El-Baghdady, Y.R.M.; Hemeida, N.A.

doi:10.1016/0378-4320(93)90095-9

Cited by 12 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1976; Amann et al. 1987; Abou‐Ahmed et al. 1993) and in the maintenance of motility during storage (Padilla and Foote 1991; Braun et al.…”

Section: Discussionmentioning

confidence: 99%

“…As expected, variation between stallions was clearly evident. Previous reports have shown significant differences between stallions both in chemical characteristics of seminal plasma (Gebauer et al 1976;Amann et al 1987;Abou-Ahmed et al 1993) and in the maintenance of motility during storage (Padilla and Foote 1991;Braun et al 1994). Individual differences in the effects of seminal plasma on the quality of semen during cooled storage (Katila et al 2004), as well as after freezing and thawing (Aurich et al 1998), have been studied by exchanging seminal plasma between stallions.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Motility and Plasma Membrane Integrity of Spermatozoa in Fractionated Stallion Ejaculates after Storage

Kareskoski

Reilas

Andersson

et al. 2006

Reprod Domestic Animals

View full text Add to dashboard Cite

With the aim of investigating properties of stallion seminal plasma to eventually improve semen-handling techniques, sperm motility and plasma membrane integrity were analysed in different fractions of the ejaculates after storage. Semen was collected using a computer-controlled automated phantom that separates the ejaculates into five successive cups. Samples containing seminal plasma and skim milk extender were compared with samples stored in skim milk extender after the removal of seminal plasma by centrifugation. Fractionated ejaculates were stored cooled for 24 h after dilution with extender (Expt 1) or frozen in liquid nitrogen (Expt 2). In Expt 1, cup 1 was pre-sperm fluid, cups 2 and 3 sperm-rich fractions, and cup 4 sperm-poor fractions. In Expt 2, cups 1 and 2 were sperm-rich fractions, and cups 3 and 4 sperm-poor fractions. One sample (WE) represented the whole ejaculate in both experiments. Motility parameters were determined with a Hamilton-Thorn Motility Analyzer, and plasma membrane integrity was assessed using carboxyfluorescein diacetate and propidium iodide staining and fluorescence microscopy. The removal of seminal plasma lowered motility values, but not plasma membrane integrity, in both experiments. No significant differences between cups were observed after cooled storage. The cups differed significantly in most post-thaw motility parameters, and the sperm-rich fraction showed higher post-thaw motility than the whole ejaculate.

show abstract

“…1976; Amann et al. 1987; Abou‐Ahmed et al. 1993) and in the maintenance of motility during storage (Padilla and Foote 1991; Braun et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Motility and Plasma Membrane Integrity of Spermatozoa in Fractionated Stallion Ejaculates after Storage

Kareskoski

Reilas

Andersson

et al. 2006

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…In addition to differences in composition between ejaculate fractions, many researchers have attributed the composition of SP to season, age and the individual stallion (Mann et al., 1956; Mann, 1975; Gebauer et al., 1976; Abou‐Ahmed et al., 1993). When SP was exchanged between stallions, the SP of stallion 4 negatively affected the quality of its own and stallion 3’s sperm during storage, but did not affect the sperm of the other two stallions.…”

Section: Discussionmentioning

confidence: 99%

Effect of Seminal Plasma Fractions on Stallion Sperm Survival after Cooled Storage

Akçay

Reilas

Andersson

et al. 2006

Journal of Veterinary Medicine Series A

View full text Add to dashboard Cite

This study aimed to evaluate stallion sperm survival after 24 h of cooled storage in the presence of seminal plasma (SP) derived from the sperm-rich fractions (SRF) or sperm-poor fractions(SPF) of the ejaculate, without SP, or in the presence of SP from other stallions. Ejaculates were collected from four stallions using an automated phantom, which separated the semen into five cups. Centrifuged and washed spermatozoa from cup 2 (SRF) were mixed with skim milk extender to a concentration of 100 x 10(6) sperm/ml and then 1:1 (v/v) with SP from the stallion's own or another stallions' second (SP-SRF) or last cup (SP-SPF). Skim milk extender (K) and skim milk extender supplemented with modified Tyrode's medium (KMT) were used as control treatments. After a 24-h storage period in a transport container, spermatozoa were evaluated for motion characteristics and plasma membrane integrity by calcein acetoxymethyl (AM)/propidium iodide staining. The percentage of spermatozoa with intact plasma membranes after storage was lower in SP-SRF than in SP-SPF, and the highest in K (P < 0.05). Progressive motility (PMOT) was lower for sperm stored in SP-SRF than for sperm stored in SP-SPF (P < 0.05), but there was no significant difference in total motility (TMOT). Sperm stored in KMT (P < 0.05) registered the highest TMOT and PMOT percentages. Osmolarity was significantly higher and pH lower in K than in KMT or SP. Treatment with SP-SPF from three stallions benefited the PMOT of sperm from one stallion. These preliminary findings suggest that SP from SRFs may be more harmful during storage than SP from SPFs. Removal of SP improves sperm survival in KMT extender, and exchanging SP between stallions seems to influence sperm survival.

show abstract

“…Comparison of seminal plasma proteins among several terrestrial mammalian species (boar, bull, ram, buck, stallion, alpaca, camel) using proteomics resulted in considerable divergence among species with only three common proteins (Druart et al, 2013). While genetic diversity likely contributes to the degree of heterogeneity of seminal plasma proteins among species as reported among three different breeds of sheep (Carvajal-Serna1 et al, 2018), other factors such as seasonality reported in rams (Domínguez et al, 2008), buffalo bulls (Sharma et al, 2014), and stallions (Abou-Ahmed et al, 1993) can also have an effect on the proteinaceous constituency of seminal plasma. In the present study, perhaps the degree of heterogeneity of seminal plasma proteins among odontocetes may have been due, in part, to season since the beluga (Steinman et al, 2012) and Pacific white-sided dolphin (Robeck et al, 2009) are seasonally polyestrous.…”

Section: Discussionmentioning

confidence: 99%

Preliminary Evaluation of Seminal Plasma Proteins and Immunoreactivity of Nerve Growth Factor as Indicative of an Ovulation Inducing Factor in Odontocetes

et al. 2019

View full text Add to dashboard Cite

In the seminal plasma of terrestrial mammalian species known as induced (e.g., camels) and spontaneous (e.g., cattle) ovulators, an ovulation-inducing factor (OIF) with a protein structure similar to beta-nerve growth factor (β-NGF) has been identified. Detection of an OIF/NGF in the seminal plasma of cetaceans would have both basic and applied implications in reproductive biology and conservation management programs. A preliminary evaluation was conducted to characterize the distribution and abundance of seminal plasma proteins in aquarium-based belugas and a Pacific white-sided and bottlenose dolphin. Initially, SDS-PAGE was used with 50 µg of total protein for separation; thereafter, Western immunoblot was used with anti-NGF. In addition to odontocete seminal plasma, a purified fraction of llama seminal plasma (100 ng protein) and an extract of mouse brain (20 µg total protein) were included as positive controls for NGF. Within the two belugas, visual inspection of the protein bands indicated similar distribution and intensity. However, among the belugas and Pacific white-sided and bottlenose dolphins there was more diversity than similarity in the distribution and abundance of seminal plasma proteins. While immunoreactivity of NGF was distinctly evident in the llama and mouse positive controls, there was no visual reactivity in any of the odontocete samples. These preliminary results provide novel information indicating more homogeneity within and heterogeneity among seminal plasma proteins of ondentocetes. Although NGF was not immunologically detected, future studies are required to address the apparent limitations of immuno-detection of NGF, especially if the post-translational form of β-NGF is in low abundance in the seminal plasma of belugas and Pacific white-sided and bottlenose dolphins.

show abstract

Influence of age and season on certain biochemical constituents of seminal plasma of Arabian horses

Cited by 12 publications

References 14 publications

Motility and Plasma Membrane Integrity of Spermatozoa in Fractionated Stallion Ejaculates after Storage

Motility and Plasma Membrane Integrity of Spermatozoa in Fractionated Stallion Ejaculates after Storage

Effect of Seminal Plasma Fractions on Stallion Sperm Survival after Cooled Storage

Preliminary Evaluation of Seminal Plasma Proteins and Immunoreactivity of Nerve Growth Factor as Indicative of an Ovulation Inducing Factor in Odontocetes

Contact Info

Product

Resources

About