Influence of age and genetic background on in vivo fatty acid synthesis in obese (ob/ob) mice

Lipogenic capacity and relative contribution of the different tissues and organs to lipid synthesis in male rat

“…In the latter work, mesenteric white adipose tissue was not separated from the intestines. However, this adipose tissue has remarkable lipogenic capacity (our results, Patkins and Masoro, 1964 ;Hems, Rath and Verrinder, 1975) The rates of total lipid and fatty acid synthesis (per g of tissue) were, on the average, much lower than the rate measured in the liver, confirming previous results (Hems, Rath and Verrinder, 1975 ;Cornish and Cawthorne, 1978 ;Rath and Thenen, 1980 ;Stansbie et al, 1976 Favarger and Gerlach (1955) using 14 C precursor ; (2) results comparable to ours were obtained by Godbole and York (1978) using intravenous injection of 3 H 2 0 and by Patkins and Masoro (1964) using per os administration of !4C-glucose ; (3) in vitro observations (Gandemer, Pascal and Durand, 1979 ;Benjamin et al, 1971) (Favarger, 1965) (Jungas, 1968). From data on table 1, we can calculate that the rate of 3 H incorporation into glycerol in the whole of the white adipose tissues was 176 !g of 3 H/10 min (640 -464 = 176).…”

“…Although there are no comparative data in the literature on organ contribution to lipid synthesis in rat, a study in mouse has shown that kidneys, lungs, brain and heart synthesize few fatty acids (Hollands and Cawthorne, 19811. Our estimate of the contribution of intestines to lipid synthesis in rat is close to that found in mouse by Rath and Thenen (1980) but much lower than reported by Hollands and Cawthorne (1981) who attributed to intestines a role equivalent to that of liver. In the latter work, mesenteric white adipose tissue was not separated from the intestines.…”

“…The above studies showed the need of using such a precursor as tritiated water (Jungas, 1968 ;Lowenstein, 1971) (Yen et al, 1976 ;Le Marchand-Brustel and Jeanrenaud, 1978 ;Cawthorne and Cornish, 1979 ;Cornish and Cawthorne, 1978 ;Rath and Thenen, 1980 ;Hollands and Cawthorne, 1981 ;Romsos and Leveille, 1974) and rat (Stansbie et al, 1976 ;Lakshmanan, Berdanier and Veech, 1977 ;Godbole and York, 1978 ;Kannan et al, 1980) (Baker, Learn and Bruckdorfer, 1978 ;Cawthorne and Cornish, 1979 ;Cornish and Cawthorne, 1978 ;Romsos and Leveille, 1974), genetics (Yen et al, 1976 ;Le Marchand-Brustel and Jeanrenaud, 1978 ;Cawthorne and Cornish, 1979 ;Rath and Thenen, 1980 ;Lakshmanan, Berdanier and Veech, 1977) and physiology (Le Marchand-Brustel and Jeanrenaud, 1978 ;Cornish and Cawthorne, 1978 ;Rath and Thenen, 1980), but all data indicate that the rest of the carcass is the main site of lipid synthesis. Of the many tissues in the carcass, only white adipose tissues have been thoroughly studied (Hems, Rath and Verrinder, 1975 ;Cornish and Cawthorne, 1978 ;Rath and Thenen, 1980 ;Hollands and Cawthorne, 1981 ;Stansbie et a/., 1976 ;Godbole and York, 1978 (Le Marchand-Brustel and Jeanrenaud, 1978 ;Rath and Thenen, 1980) and (2) the principal tissues and organs (White adipose tissues, skin, carcass, liver) are large enough so that the specific radioactivity of their total lipids and fatty acids can measured individua...…”

“…The animal was skinned and dissected rapidly. The (Yen et al, 1976 ;Rath and Thenen, 1980 ;Lakshmanan, Berdanier and Veech, 1977 (Cawthorne and Cornish, 1979 ;Romsos and Leveille, 1974).…”

Regulation of Fasting Fuel Metabolism by Toll-Like Receptor 4

“…Several studies around the 1970s showed that liver and dissectible adipose tissue usually contribute to no more than 40% of total fatty acid synthesis rate in rodents. Instead, the rest of carcass, mainly muscle and skeleton, is the major site of fatty acid synthesis (27–32). Although there was one study that suggested that the intermuscular fat in carcass might contribute to lipogenesis (33), several following studies using dissected muscle without fat showed that muscle tissue may be an important lipogenic tissue because of its large mass (27,34,35).…”