Lipogenic capacity and relative contribution of the different tissues and organs to lipid synthesis in male rat

Gandemer, G.; Pascal, G.; Durand, Georges

doi:10.1051/rnd:19830410

Cited by 27 publications

(14 citation statements)

References 25 publications

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“…Briefly, the tissues were homogenised in 0.25 M sucrose and centrifuged at 40000 g for 40 min. The supernatant cytosolic fractions were analysed for malic enzyme (ME, EC 1.1.1.40) and glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) using modifications [43] of the methods of Fitch et al [44] and Hsu and Lardy [45], respectively. Acetyl-CoA-carboxylase (ACC, EC 6.4.1.2) was assayed by the H 14 CO 3 -fixation method [46,47], using H 14 CO 3 (NEN, Life Science Product, Boston, USA, 49 mCi·mmol -1 ).…”

Section: Lipogenic Enzymesmentioning

confidence: 99%

Dose effect of alpha-linolenic acid on lipid metabolism in the hamster

Morise¹,

Mourot²,

Riottot³

et al. 2005

Reprod. Nutr. Dev.

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-In order to meet dietary requirements, the consumption of α-linolenic acid (ALA, 18:3 n-3) must be promoted. However, its effects on triglyceride (TG) and cholesterol metabolism are still controversial, and may be dose-dependent. The effects of increasing dietary ALA intakes (1%, 10%, 20% and 40% of total FA) were investigated in male hamsters. ALA replaced oleic acid while linoleic and saturated FA were kept constant. Triglyceridemia decreased by 45% in response to 10% dietary ALA and was not affected by higher intakes. It was associated with lower hepatic total activities of acetyl-CoA-carboxylase (up to -29%) and malic enzyme (up to -42%), which were negatively correlated to ALA intake (r 2 = 0.33 and r 2 = 0.38, respectively). Adipose tissue lipogenesis was 2-6 fold lower than in the liver and was not affected by dietary treatment. Substitution of 10% ALA for oleic acid increased cholesterolemia by 15% but, as in TG, higher ALA intakes did not amplify the response. The highest ALA intake (40%) dramatically modified the hepatobiliary metabolism of sterols: cholesterol content fell by 45% in the liver and increased by 28% in the faeces. Besides, faecal bile acids decreased by 61%, and contained more hydrophobic and less secondary bile acids. Thus, replacing 10% oleic acid by ALA is sufficient to exert a beneficial hypotriglyceridemic effect, which may be counteracted by the slight increase in cholesterolemia. Higher intakes did not modify these parameters, but a very high dose resulted in adverse effects on sterol metabolism. α-linolenic acid / oleic acid / lipogenesis / cholesterol / triglyceride / bile acid / hamster

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Section: Lipogenic Enzymesmentioning

confidence: 99%

Dose effect of alpha-linolenic acid on lipid metabolism in the hamster

Morise¹,

Mourot²,

Riottot³

et al. 2005

Reprod. Nutr. Dev.

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“…To measure the activity of malic enzyme (EC 1.1.1.40), samples of muscle (1.5 g) and fat (0.7 g) were homogenized in 0.25 mol/L sucrose buffer and centrifuged at 30,000 × g for 40 min at 4°C. Supernatants were analyzed for malic enzyme using a modified (Gandemer et al, 1983) method of Hsu and Lardy (1969). Malic enzyme was assayed through measurement of NADPH formation at 37°C by absorbance at 340 nm.…”

Section: Meat and Fat Qualitymentioning

confidence: 99%

Influence of extruded linseed on growth, carcass composition, and meat quality of slaughtered pigs at one hundred ten and one hundred sixty kilograms of liveweight1

Corino

Musella

Mourot

2008

Journal of Animal Science

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ABSTRACT:The Western diet is characterized by a high intake of SFA relative to PUFA, and the consumption of n-3 PUFA is decreased relative to n-6 PUFA. Therefore, there has been much interest in recent years in ways to manipulate the fatty acid composition of meat. The objective of this work was to determine the effects of dietary extruded linseed, an n-3 PUFA source, on growth performance, carcass composition, meat quality, and oxidative stability of pigs slaughtered at 111.0 (±4.8) kg of BW and 160.0 (±9.2) kg of BW. The association of these factors with BW at slaughter was also examined. Forty barrows, 78.1 (±1.75) kg of initial BW, were fed a control diet (2.5% sunflower oil) or a linseed diet containing 5% of whole extruded linseed. Both diets contained 170 mg of vitamin E and 250 μg of selenium. Eight pigs from each dietary treatment were slaughtered at 110 kg of BW and the others at 160 kg of BW. There was no dietary effect (P > 0.05) on growth, carcass characteristics, meat quality, or the activity of malic enzyme in LM and backfat. Inclusion of linseed increased (P < 0.05) n-3 PUFA content in both LM and backfat and decreased the n-6:n-3 PUFA ratio from 12 to 4.5 in LM, and from 11 to 3 in backfat. Liveweight at slaughter significantly influenced carcass characteristics, meat quality, total lipid and oxidative stability of LM, malic enzyme activity in adipose tissue, and fatty acid content of LM and backfat. This study shows that the inclusion of linseed in swine diets may improve the fatty acid profile of pork without deleteriously affecting oxidation or color stability. Thus, such a feeding practice may improve human health based on the n-6:n-3 PUFA ratio recommended for the human diet.

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“…The supernates were analyzed for malic enzyme (ME, EC 1.1.1.40) and glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) activities using a modification (Gandemer et al, 1983) of the methods of Hsu and Lardy (1969) and Fitch et al (1959). The formation of NADPH was measured at 37°C by absorbance at 340 nm.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Influence of dietary conjugated linoleic acid on growth, meat quality, lipogenesis, plasma leptin and physiological variables of lipid metabolism in rabbits1

Corino

Mourot²,

Magni

et al. 2002

Journal of Animal Science

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ABSTRACT:We investigated the effects of conjugated linoleic acid (CLA) supplementation on growth, feed efficiency, carcass characteristics, meat quality, lipogenesis, and lipid metabolism in rabbits. One hundred forty-four New Zealand White rabbits, half males, half females, age 55 d, mean 1.8 kg BW, were randomly assigned to three weight-and sex-balanced feeding groups in which conventional pelleted diets were supplemented with 0, 0.25, or 0.5% of a CLA preparation. The CLA preparation contained 65% CLA isomers. Twelve rabbits (six males and six females from each group) were slaughtered at each of three slaughtering trials (2.5, 2.8, and 3.1 kg BW, or 76, 90, and 104 d of age). Conjugated linoleic acid supplementation did not influence growth performance (P ≥ 0.05) or carcass characteristics but reduced perirenal fat at heavier slaughtering weights (P = 0.09 at 2.8 kg BW; P < 0.01 at 3.1 kg BW). Conjugated linoleic acid reduced acetyl-CoAcarboxylase (CBX) activity in liver (P < 0.05) and adi-

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Lipogenic capacity and relative contribution of the different tissues and organs to lipid synthesis in male rat

Abstract: Introduction.

Cited by 27 publications

References 25 publications

Dose effect of alpha-linolenic acid on lipid metabolism in the hamster

Dose effect of alpha-linolenic acid on lipid metabolism in the hamster

Influence of extruded linseed on growth, carcass composition, and meat quality of slaughtered pigs at one hundred ten and one hundred sixty kilograms of liveweight1

Influence of dietary conjugated linoleic acid on growth, meat quality, lipogenesis, plasma leptin and physiological variables of lipid metabolism in rabbits1

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