Influence of a loblolly pine (Pinus taeda L.). Culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.)

Abstract

A new culture medium, originally designed and shown to grow cell suspensions from a variety of loblolly pine (Pinus taeda L.) explants, was used to study growth and somatic embryogenesis of the wild carrot (Daucus carota L.) in cell suspensions. The new loblolly pine medium (LM) differed from the standard wild carrot medium (WCM) in having very low Ca(2+), very high Mg(2+), and enrichment with PO inf4 (sup3-) and microelements. When WCM was altered to contain levels of Ca(2+) or Ca(2+) and Mg(2+) equivalent to LM, it supported neither growth nor embryogenesis of the wild carrot. However, growth and embryogenesis in LM was superior to WCM. The phosphate level in WCM was found to be suboptimal.

Somatic embryogenesis of selected spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana)

“…77, No. 3: 189-199, 2008 195 ACTA SOCIETATIS BOTANICORUM POLONIAE (Litvay et al 1985), containing 1/2 of nutrient concentrations of LM medium.…”

“…In this study, two basal media were tested: BM-3 (Gupta and Durzan 1986) and LM (Litvay et al 1985), either fullstrength or half-strength. The explants were cultured on three variants of the media: (a) full-strength BM-3 and LM; (b) 1/2 BM-3 and 1/2 LM (with 1/2 of full concentrations of media); and (c) 1/2 macro BM-3 and 1/2 macro LM (with 1/2 macro and full concentrations of micronutrients).…”

Effect of ascorbic acid and glutathione on somatic embryogenesis induction in Picea pungens Engelmann

“…Для инициации соматического эмбриогенеза из незрелых зиготических зародышей исполь-зовали базовые среды Дурзана (DCR) (Gupta, Durzan, 1985) и Литвая (LV) (Litvay et al, 1985) с уменьшенным вдвое содержанием макроэле-ментов. Все среды дополняли мезоинозитом -100 мг/л (Sigma-Aldrich, США), гидролизатом казеина -500 мг/л (Fluca Analytical, США), глу-таминовой кислотой -500 мг/л (Sigma-Aldrich, Сент-Луис, США) и агаром -0,7 % (PanReac®, Испания).…”

Somatic embryogenesis of selected coniferous tree species of the genera Picea, Abies and Larix

“…At all stages of somatic embryogenesis following basal media were used: for spruces the BM-3 medium (Gupta and Durzan 1986) and 50% LM medium (Litvay et al 1985); for larches the MSG medium (Becwar et al 1990); and for fir the MCM medium Jansson 1981 after Hristoforoglu et al 1995). During embryogenic callus initiation, the above-mentioned BM-3 medium and the SH medium (Schenck and Hildebrandt 1972) were used additionally for fir.…”

“…mZE mature zygotic embryo; iZE immature zygotic embryo; M megagametophyte (endosperm with immature zygotic embryo); SE somatic embryo; C and H cotyledons and hypocotyls of 1-month-old somatic seedlings. Media: BM-3 (Gupta and Durzan 1986), LM (Litvay et al 1985), MSG (Becwar et al 1990), MCM Jansson 1981 after Hristoforoglu et al 1995). Proliferation and development of embryogenic tissue Embryogenic tissues of individual tree species differed in survival rate and potential for further proliferation.…”