Effect of ascorbic acid and glutathione on somatic embryogenesis induction in Picea pungens Engelmann

doi:10.14258/turczaninowia.20.3.4

Cited by 4 publications

(3 citation statements)

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“…To date, only a few publications have shown at the physiological level that ASA may be involved in promoting the SE process in conifers ( Stasolla and Yeung, 2007 ; Pullman et al., 2015 ; Zheleznichenko and Novikova, 2017 ). According to Zheleznichenko and Novinkova ( Zheleznichenko and Novikova, 2017 ), this antioxidant stimulates the initial steps of somatic embryo development in Picea pungens Engelmann. Stasolla and Yeung (2007) reported a positive effect on somatic embryo formation in Picea glauca (Moench) Voss.…”

Section: Discussionmentioning

confidence: 99%

“… Stasolla and Yeung (2007) reported a positive effect on somatic embryo formation in Picea glauca (Moench) Voss. The induction and proliferation stages of P. pungens were promoted even when the basal medium was supplemented with 300 mg l −1 ASA ( Zheleznichenko and Novikova, 2017 ). On the other hand, Malabadi and Van Staden (2005) reported a negative effect of ASA at a concentration of 0.01%–0.5% on SE induction from vegetative shoot apices of Pinus patula .…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have reported that ascorbic acid (ASA) can have a positive effect on SE in spruce at the physiological level ( Stasolla and Yeung, 2007 ; Zheleznichenko and Novikova, 2017 ). However, they mainly refer to the maturation and germination stages of the somatic embryos.…”

Section: Introductionmentioning

confidence: 99%

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Modulating ascorbic acid levels to optimize somatic embryogenesis in Picea abies (L.) H. Karst. Insights into oxidative stress and endogenous phytohormones regulation

Hazubska-Przybył,

Obarska,

Konecka

et al. 2024

Front. Plant Sci.

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Global warming has adversely affected Picea abies (L.) H. Karst. forests in Europe, prompting the need for innovative forest-breeding strategies. Somatic embryogenesis (SE) offers promise but requires protocol refinement. Understanding the molecular mechanisms governing somatic embryo development is essential, as oxidative stress plays a crucial role in SE regulation. Ascorbic acid (ASA), is a vital antioxidant that can potentially control oxidative stress. In the present study, we normalized ASA concentrations in induction and proliferation media to enhance embryogenic tissue (ET) regeneration and proliferation capacity of mature explants. The media were supplemented with ASA at 0 mg l−1, 25 mg l−1, 50 mg l−1, 100 mg l−1, and 200 mg l−1. The accumulation of hydrogen peroxide (H2O2) and endogenous phytohormones, including auxins, cytokinins, brassinosteroids, abscisic acid, and gibberellin, was measured in non-embryonic calli and ET. Subsequently, their impact on ET induction and multiplication was analyzed. Our results demonstrate that application of ASA at concentrations of 25 mg l−1 and 200 mg l−1 led to increased H2O2 levels, potentially inducing oxidative stress while simultaneously reducing the levels of all endohormone groups. Notably, the highest ET induction frequency (approximately 70%) was observed for ASA at 50 mg l−1. These findings will enhance SE induction procedures, particularly in more resistant explants, underscoring the significance of ASA application to culture media.

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