Influence of a 50 Hz extra low frequency electromagnetic field on spermatozoa motility and fertilization rates in rabbits

Roychoudhury, Shubhadeep; J, Jedlicka; Parkányi, V.; Rafay, J.; Ondruška, Ľubomír; Massányi, Peter; Bulla, J.

doi:10.1080/10934520902997029

Cited by 42 publications

(44 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ELF-EMFs have been found to produce a variety of biological effects, from simple enzyme reactions to the far more complex gene induction and protein [Goodman and Blank, 1998;De Mattei et al, 2005;Delle Monache et al, 2008;Piacentini et al, 2008;Goodman et al, 2009]. Recently, studies carried out on mammalian sperm suggest that ELF-EMFs can negatively [Bernabò et al, 2007] or positively [Iorio et al, 2007;Roychoudhury et al, 2009] influence sperm motility according to the specific characteristics of the EMF applied and/or to the type of sperm used. In humans, the exposure of spermatozoa to an ELF-EMF with a square waveform of 5 mT amplitude and frequency of 50 Hz resulted in an increase in motility and kinematic parameters [Iorio et al, 2007].…”

Section: Introductionmentioning

confidence: 98%

Involvement of mitochondrial activity in mediating ELF‐EMF stimulatory effect on human sperm motility

Iorio

Monache

Bennato

et al. 2010

Bioelectromagnetics

View full text Add to dashboard Cite

It has recently been reported that the exposure of human spermatozoa to an extremely low frequency (ELF) electromagnetic field (EMF) with a square waveform of 5 mT amplitude and frequency of 50 Hz improves sperm motility. The functional relationship between the energy metabolism and the enhancement of human sperm motility induced by ELF-EMF was investigated. Sperm exposure to ELF-EMF resulted in a progressive and significant increase of mitochondrial membrane potential and levels of ATP, ADP and NAD(+) that was associated with a progressive and significant increase in the sperm kinematic parameters. No significant effects were detected on other parameters such as ATP/ADP ratio and energy charge. When carbamoyl cyanide m-chlorophenylhydrazone (CICCP) was applied to inhibit the oxidative phosphorylation in the mitochondria, the values of energy parameters and motility in the sperm incubated in the presence of glucose and exposed to ELF-EMF did not change, thus indicating that the glycolysis was not involved in mediating ELF-EMF stimulatory effect on motility. By contrast, when pyruvate and lactate were provided instead of glucose, the energy status and motility increased significantly in ELF-EMF-treated sperm. Under these culture conditions, the inhibition of glycolitic metabolism by 2-deoxy-D-glucose (DOG) again resulted in increased values of energy and kinematic parameters, indicating that gluconeogenesis was not involved in producing glucose for use in glycolysis. We concluded that the key role in mediating the stimulatory effects exerted by ELF-EMF on human sperm motility is played by mitochondrial oxidative phosphorylation rather than glycolysis.

show abstract

Section: Introductionmentioning

confidence: 98%

Involvement of mitochondrial activity in mediating ELF‐EMF stimulatory effect on human sperm motility

Iorio

Monache

Bennato

et al. 2010

Bioelectromagnetics

View full text Add to dashboard Cite

show abstract

“…We have previously reported the lack of alterations on morphological characteristics of male germ cells in mice exposed to a 60 Hz and 2.0 mT magnetic fields (Heredia-Rojas et al, 2004). In contrast, Roychoudhury et al (2009) found alterations of spermatozoa and fertilization rates in rabbits exposed to 50 Hz magnetic fields. For humans, it is accepted that there is evidence demonstrating that magnetic field exposure may have adverse effects on sperm quality (Li et al, 2010).…”

Section: Discussionmentioning

confidence: 82%

Cytological effects of pulsed electromagnetic fields and static magnetic fields induced by a therapeutic device on <i>in vivo</i> exposed rats

Fuente¹,

Heredia-Rojas²,

Carranza-Rosales³

et al. 2015

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

Background: There is a trend towards the use of magnetic fields in medicine. Pulsed electromagnetic fields (PEMFs) technology was based upon 20 years of fundamental studies on the electromechanical properties of bone and other connective tissues. More recently, these magnetic fields have been used to treat several health conditions. There remains continuing concern that exposure to electromagnetic devices may cause adverse effects. The aim of the present study was to investigate the cytological effects induced in rats exposed in a patented medical device that uses PEMFs combined with static magnetic fields (SMFs). Material and Methods: Thirty sexually mature 14-week-old male and female Sprague Dawley rats were distributed into three groups: (a) 5 males and 5 females (independently) exposed to PEMFs combined with SMFs, (b) animals treated with SMFs only, and (c) non-exposed animals. Acridine orange fluorescent-staining micronucleus test and male germ cells analysis were performed according to standardized techniques. Results: A lack of evidence for alterations on micronucleus frequency, on polychromatic erythrocytes percentage, and on sperm counts and morphological characteristics of male germ cells were found in mature rats exposed to PEMFs medical device compared to non-exposed animals.Conclusions: This study suggests that the applied magnetic field generated in a therapeutic device did not have any detectable cytotoxic or genotoxic effect in exposed rats. In view of these findings and the contradictory reports in the literature, it is necessary to carry out more research to help clarify the controversy concerning cytogenotoxic risk associated with therapeutic magnetic fields exposures.

show abstract

“…A large body of evidence has confirmed that exposure to ELF-EMFs can induce spermatogenic cell apoptosis, decrease the numbers of spermatogenic cells and significantly increase abnormalities in cells. 5 However, the underlying molecular mechanism of this influence remains unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have confirmed that ELF-EMFs can alter the reproductive endocrine hormones and decrease the semen quality of humans and animals, as well as gonadal fetal function. [3][4][5] Despite numerous attempts, the biological mechanism facilitating the effects of ELF-EMFs remains unknown. Therefore, it is necessary to investigate and understand the potential effects of ELF-EMFs on the male reproductive system.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of miR-26b-5p regulates the cell cycle by targeting CCND2 in GC-2 cells under exposure to extremely low frequency electromagnetic fields

Liu

et al. 2016

Cell Cycle

View full text Add to dashboard Cite

The increasing prevalence of extremely low frequency electromagnetic fields (ELF-EMFs) exposure has raised considerable public concern regarding the potential hazardous effects of ELF-EMFs on male reproductive function. Increasing evidence indicates that miRNAs are necessary for spermatogenesis and male fertility. However, the regulation of miRNA expression and the roles of miRNAs in response to ELFEMFs remain unclear. In our study, mouse spermatocyte-derived GC-2 cells were intermittently exposed to a 50 Hz ELF-EMF for 72 h (5 min on/10 min off) at magnetic field intensities of 1 mT, 2 mT and 3 mT. MiR26b-5p was differentially expressed in response to different magnetic field intensities of ELF-EMFs. The host gene CTDSP1 showed an unmethylation status in GC-2 cells at different magnetic field intensities of ELF-EMF exposure. MiR-26b-5p had no significant, obvious influence on the cell viability, apoptosis or cell cycle of GC-2 cells. However, the overexpression of miR-26b-5p significantly decreased the percentage of G0/G1 phase cells and slightly increased the percentage of S phase cells compared to the sham group that was exposed to a 50 Hz ELF-EMF. Computational algorithms identified Cyclin D2 (CCND2) as a direct target of miR-26b-5p. MiR-26b-5p and a 50 Hz ELF-EMF altered the expression of CCND2 at both the mRNA and protein levels. Overexpressed miR-26b-5p in GC-2 cells can change the mRNA expression of CCND2 following 50 Hz ELF-EMF at 3 mT. These findings demonstrate that miR-26b-5p could serve as a potential biomarker following 50 Hz ELF-EMF exposure, and miR-26b-5p-CCND2-mediated cell cycle regulation might play a pivotal role in the biological effects of ELF-EMFs.

show abstract

Influence of a 50 Hz extra low frequency electromagnetic field on spermatozoa motility and fertilization rates in rabbits

Cited by 42 publications

References 22 publications

Involvement of mitochondrial activity in mediating ELF‐EMF stimulatory effect on human sperm motility

Involvement of mitochondrial activity in mediating ELF‐EMF stimulatory effect on human sperm motility

Cytological effects of pulsed electromagnetic fields and static magnetic fields induced by a therapeutic device on <i>in vivo</i> exposed rats

Overexpression of miR-26b-5p regulates the cell cycle by targeting CCND2 in GC-2 cells under exposure to extremely low frequency electromagnetic fields

Contact Info

Product

Resources

About