Influence of 2‐phenylethanol and 1,1′‐dimethylphenylethanol on metabolic activity and cell membrane function in ehrlich ascites tumour cells

Synthesis and Evaluation of the Antioxidant Activity of Lipophilic Phenethyl Trifluoroacetate Esters by In Vitro ABTS, DPPH and in Cell-Culture DCF Assays

“…The safety of phenethyl alcohol has recently been assessed by the Cosmetic Ingredient Review (CIR) Expert Panel and the Food and Drug Administration (FDA) [ 23 ], which included it in the list of substances Generally Recognized As Safe (GRAS) [ 24 ]. In addition, it shows antimicrobial [ 25 ], antiviral [ 26 ] and antitumor effects [ 27 ]. The hydroxylated derivatives tyrosol 4 , homovanillyl alcohol 5 , and hydroxytyrosol 6 are low-weight molecular phenols present in extra-virgin olive oil and olive oil by-products [ 28 ], well known for their beneficial health effects.…”

Chemical modification of Ehrlich ascites tumour cells by periodate and succinic anhydride: Effects on metabolism and membrane permeability

“…120. regulatory functions• The discrepancy between inhibition of glycolysis and of O 2-consumption with succinic anhydride permits an additional explanation: Succinate formed by hydrolysis may cross the cell membrane and serve as metabolic substrate for intact mitochondria; only high concentrations would abolish O2-u ptake by raising the intracdlular concentration of succinic anhydride to levels which alter the mitochondrial membrane system• The view that the cell membrane is the primary site of action of the reagents might be supported by studies with cell-free homogenates. Unfortunately, lysates from EAT ceils are very poor candidates for metabolic investigations [ 12]. Similar studies with hemolysates [9,10] and lyophilized yeast preparations [ 11 ], however, favour the assumption• Nonspecific oxidation by periodate, especially of essential sulfhydryl groups, could largely be excluded by studies with sulfhydryl reagent sensitive enzymes• Furthermore, our results with cell-free systems indicate for both reagents that intracellular reactions can play only a subordinate role in metabolic inhibition of whole cells.…”

“…With both reagents inhibitory effects of reaction products (formaldehyde, formate, iodate, succinate) could be excluded. The methods for the determination of metabolic rates, potassium and enzyme leakage, and of cell viability have been described [ 12]. In some experiments we used a new titrimetric method to measure O2-consumption.…”