Influence of 2,4-D and BAP on callus growth and the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium x domesticum cv. Madame Layal

Haensch, Klaus-Thomas; Schulz, Philip

doi:10.2225/vol10-issue1-fulltext-9

Cited by 9 publications

(12 citation statements)

References 12 publications

(25 reference statements)

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“…Hasil dari perlakuan C terbentuk kalus, kalus dapat dijadikan planlet jika di ganti media pertumbuhannya yang mengandung akusin dan sitokinin dengan kombinasi yang sesuai (Haensch, 2007). Perlakuan A merupakan media perlakuan yang ideal untuk pembentukan planlet, oleh karena itu, kalus dari media C ditransfer ke media perlakuan A.…”

Section: Pembentukan Planlet Dari Kalus Bacopa Australisunclassified

“…Pembentukan kalus memiliki tujuan penting yaitu untuk mendapatkan lebih banyak planlet, karena setiap sel kalus memiliki potensi pertumbuhan planlet (Haensch, 2007). Pada penelitian ini sel somatik dibuat secara tidak langsung melalui proses pembentukan kalus.…”

Section: Pembentukan Planlet Dari Kalus Bacopa Australisunclassified

“…Haensch (2007) melaporkan regenerasi pembentukan planlet dari kalus pada tanaman Pelargonium x domesticum cv. Madame Layal dengan BAP terjadi dalam empat minggu.…”

Section: Pembentukan Planlet Dari Kalus Bacopa Australisunclassified

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Pembentukan mother plant Bacopa australis secara In-vitro dan aklimatisasi dalam aquascape air tawar

Nugraha¹,

Yunita²,

Lestari³

et al. 2017

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ABSTRAKTanaman air adalah bagian penting dari ekosistem air tawar. Salah satu spesies yang terkenal adalah Bacopa australis. Hobiis aquascape saat ini memiliki ketertarikan tinggi terhadap tanaman air dengan kualitas yang bagus dari setiap spesiesnya. Metode perbanyakan tanaman air tanpa tanah, lahan pertanian dan air perlu dilakukan untuk memenuhi keinginan tersebut. Tujuan dari penelitian ini adalah untuk mendapatkan formula media kultur jaringan dan zat pengatur tumbuh yang tepat untuk multiplikasi dalam perakitan mother plant (tanaman induk) Bacopa australis, serta mendapatkan media terbaik untuk aklimatisasi. Media yang digunakan adalah media Murashige dan Skoog (MS) A padat dengan perbedaan konsentrasi zat pengatur tumbuh. Perlakuan uji dalam kombinasi zat pengatur tumbuh (a) 0,50 mg/L BAP + 0,50 mg/L kinetin; (b) 0,50 mg/L BAP; dan (c) 0,50 mg/L 2,4-D. Aklimatisasi tanaman induk dilakukan pada berbagai media antara lain 1) pasir silika + pupuk aqua soil amazonia, 2). pasir malang + pupuk aqua soil amazonia, 3) pasir silika + pupuk cair; 4) pasir malang + pupuk. Hasil yang diperoleh, yaitu formula media kultur terbaik untuk multiplikasi tunas tanaman B. australis secara in-vitro adalah media MS (A) yang diperkaya dengan 0,5 mg/L BAP + 0,5 mg/L kinetin, sedangkan aklimatisasi terbaik pada media pasir malang + pupuk aqua soil amazonia.KATA KUNCI: Bacopa australis; in-vitro; tanaman induk; zat pengatur tumbuh; media aklimatisasi ABSTRACT:The

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Section: Pembentukan Planlet Dari Kalus Bacopa Australisunclassified

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Pembentukan mother plant Bacopa australis secara In-vitro dan aklimatisasi dalam aquascape air tawar

Nugraha¹,

Yunita²,

Lestari³

et al. 2017

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“…Kombinasi antara 2,4-D dan BAP pada media induksi kalus berperan dalam induksi sifat-sifat embriogenesis somatik, misal struktur bipolar melalui regulasi arah morfogenesis dan menginduksi pertumbuhan kalus melalui proliferasi (Haensch, 2007;George et al, 2008).…”

Section: Pendahuluanunclassified

Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang)

Azizah¹,

Restanto²,

Sugiharto³

2017

J. I. Dasar

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Indica rice variety Ciherang is the most planted variety in Indonesia, but the micropropagation technique is restricted because it is known has low regeneration frequency and included as recalcitrant cultivar for tissue culture and tranformation activities. One of solution to resolve that problem is developing a technique of somatic embryogenesis in callus of ciherang rice cultivar. The aims of study were to determine medium composition for inducting embryogenic callus in Ciherang rice and to know the effectivity of rice regeneration using callus as explant. The methods were included induction of embryogenic callus in callus induction media (CIM) containing MS basal, Proline 600 mg/l, Casein Hidrolisat 300 mg/l, phytagel 2,5 g/l, BAP 0.25 mg/l, sukrosa 30 %, and 2,4-D in different concentration, from 2,4-D 0 mg/l as control (CIM 1), 2,4-D 2 mg/l (CIM 2), 2,4-D 3 mg/l (CIM 3), and 2,4-D 4 mg/l (CIM 4). It then be continued to regenerate the calli in RM 1 medium containing MS basal, NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 10 g/l, and sukrosa 30 %, pH 5,8 for six days in dark and RM2 medium containing MS basal , NAA 0,2 mg/l, Kinetin 2 mg/l, Agarose 8 g/l, sukrosa 30 %, pH 5,8 in light room. Results showed CIM 4 medium using 2,4-D 4 mg/l gave optimum result in calli induction with procentage 57,63% and CIM 3 using 2,4-D 3 mg/l gave optimum result in embryonic calli induction with procentage 53,63%. Result of embryonic calli gave regeneration frequency procentage is 9,6%. The total planlet obtained after five weeks old in regeneration medum were 11 planlets ready for aclimatization.

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“…The majority of regeneration systems for Pelargonium species reported in the literature have focused on Zonal (Pelargonium 9 hortorum), Regal (Pelargonium 9 domesticum) and Ivy-leaf pelargoniums (Pelargonium peltatum), (Mithila et al 2001;Haensch 2007), compared to fewer reports for scented P. capitatum and P. graveolens (Rao 1994;KrishnaRaj et al 1997;Saxena et al 2000;Hassanein and Dorion 2005). Regeneration in different species of Pelargonium has been studied from diverse starting material, either by indirect organogenesis (Abo El Nil et al 1976, Dunbar andStephens 1989) or by adventitious shoot organogenesis (Boase et al 1998, Agarwal andRanu 2000).…”

Section: Introductionmentioning

confidence: 99%

Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars

Arshad

Silvestre

Merlina

et al. 2011

Plant Cell Tiss Organ Cult

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Shoot organogenesis from mature leaf tissues of two scented Pelargonium capitatum cultivars, 'Attar of Roses' and 'Atomic Snowflake', grown in the greenhouse, were optimized in the presence of thidiazuron (TDZ). The protocol involved preculture of leaf sections on basal Murashige and Skoog (MS) medium supplemented with 10 lM TDZ, 4.4 lM of 6-benzyladenine (BA) and 5.4 lM a-naphtaleneacetic acid (NAA) for a period of 2 weeks and followed by subculture of explants to a fresh medium containing 4.4 lM BA and 5.4 lM NAA. Frequency of regeneration reached approximately 93% for both cultivars, with the induction of more than 100 shoots per explant. Regenerated plantlets were rooted on half-strength MS medium supplemented with 4.4 mM sucrose and 8.6 lM of Indole-3-acetic acid (IAA). All regenerated shoots from both cultivars developed roots when transferred to organic soil mix, acclimatized, and successfully transferred to greenhouse conditions. When regenerated shoots were transferred to hydroponic conditions, frequency of survival was 76.2 and 61.9% for 'Attar of Roses' and 'Atomic Snowflake', respectively.

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Influence of 2,4-D and BAP on callus growth and the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium x domesticum cv. Madame Layal

Cited by 9 publications

References 12 publications

Pembentukan mother plant Bacopa australis secara In-vitro dan aklimatisasi dalam aquascape air tawar

Pembentukan mother plant Bacopa australis secara In-vitro dan aklimatisasi dalam aquascape air tawar

Enhancement of Regeneration Efficiency through Callus Induction Media Using 2,4-Dicholorophenoxyacetic acid in Indica Rice (Oryza sativa L. var. Ciherang)

Thidiazuron-induced shoot organogenesis from mature leaf explants of scented Pelargonium capitatum cultivars

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