Influence cationic and anionic PAMAM dendrimers of low generation on selected hemostatic parameters in vitro

Айсина, Р.Б.; Мухаметова, Л.И.; Ivanova, Ekaterina A.

doi:10.1016/j.msec.2019.110605

Cited by 19 publications

(9 citation statements)

References 34 publications

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“…The internalization of cationic dendrimers is more effective than anionic or neutral dendrimers [34]. It is also proven that positively charged dendrimers are more toxic than their analogs with a negative charge on the surface [35]. Cationic nanoparticles have the ability to damage the integrity of the cell membrane and can lead to the lysis of the cells by increasing their permeability [36].…”

Section: Discussionmentioning

confidence: 99%

Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells

Białkowska

Miłowska

Michlewska

et al. 2021

IJMS

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The application of siRNA in gene therapy is mainly limited because of the problems with its transport into cells. Utilization of cationic dendrimers as siRNA carriers seems to be a promising solution in overcoming these issues, due to their positive charge and ability to penetrate cell membranes. The following two types of carbosilane dendrimers were examined: CBD-1 and CBD-2. Dendrimers were complexed with pro-apoptotic siRNA (Mcl-1 and Bcl-2) and the complexes were characterized by measuring their zeta potential, circular dichroism and fluorescence of ethidium bromide associated with dendrimers. CBD-2/siRNA complexes were also examined by agarose gel electrophoresis. Both dendrimers form complexes with siRNA. Moreover, the cellular uptake and influence on the cell viability of the dendrimers and dendriplexes were evaluated using microscopic methods and XTT assay on MCF-7 cells. Microscopy showed that both dendrimers can transport siRNA into cells; however, a cytotoxicity assay showed differences in the toxicity of these dendrimers.

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Section: Discussionmentioning

confidence: 99%

Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells

Białkowska

Miłowska

Michlewska

et al. 2021

IJMS

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“…Each subsequent layer around the core is called a generation. There are functional groups on the surface of the dendrimer, the number of which increases twice with each generation [ 11 , 12 ]. Cationic PAMAM dendrimers have an ethylenediamine core from which, depending on the generation of a dendrimer, a certain number of branches terminated with amino-NH 2 groups depart.…”

Section: Introductionmentioning

confidence: 99%

Poly(amidoamine) Dendrimers as Nanocarriers for 5-Fluorouracil: Effectiveness of Complex Formation and Cytotoxicity Studies

Szota

Reczyńska-Kolman

Pamuła

et al. 2021

IJMS

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Two generations of positively charged poly(amidoamine) dendrimers (PAMAMs) were selected for study as potential carriers for the anticancer drug 5-fluorouracil (5FU), a drug primarily used in the treatment of colorectal cancer. Analytical techniques, such as UV-Vis spectrophotometry, NMR Spectroscopy and Laser Doppler Velocimetry (LDV), have shown that the most critical factor determining the formation of a PAMAM–5FU complex is the starting components’ protonation degree. The tests confirmed the system’s ability to attach about 20 5FU molecules per one dendrimer molecule for the G4PAMAM dendrimer and about 25 molecules for the G6PAMAM dendrimer, which gives a system yield of 16% for the fourth generation and 5% for sixth generation dendrimers. Additionally, using the QCM-D method, the adsorption efficiency and the number of drug molecules immobilized in the dendrimer structure were determined. A new aspect in our study was the determination of the change in zeta potential (ζ) induced by the immobilization of 5FU molecules on the dendrimer’s outer shell and the importance of this effect in the direct contact of the carrier with cells. Cytotoxicity tests (resazurin reduction and MTS tests) showed no toxicity of dendrimers against mouse fibroblast cells (L929) and a significant decrease in cell viability in the case of four human malignant cell lines: malignant melanoma (A375), glioblastoma (SNB-19), prostate cancer (Du-145) and colon adenocarcinoma (HT-29) during incubation with PAMAM–5FU complexes. The purpose of our work was to investigate the correlation between the physicochemical properties of the carrier and active substance and the system efficiency and optimizing conditions for the formation of an efficient system based on PAMAM dendrimers as nanocarriers for 5-fluorouracil. An additional aspect was to identify potential application properties of the complexes, as demonstrated by cytotoxicity tests.

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“…In another study, Aisina et al . [ 46 ] evaluated the effects of cationic and anionic PAMAM dendrimers on platelet concentrates. Studies on platelet bags showed that although positively charged dendrimers caused platelet aggregation and deformation in plasma, anionic dendrimers did not have such effects.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, the reported effects of polymeric nanoparticles on platelets between 2010 and 2020 were reviewed. The results of studies have shown that platelet membrane charge is negative; therefore, nanoparticles with positive charge facilitate platelet-platelet reactions by neutralizing the repulsive force between the negatively charged surfaces of cells and creating cross bridges among them, which causes platelet aggregation [ 40 , 46 , 68 , 92 ]. Prevention of platelet aggregation during storage depends on other factors, such as size, shape, molecular weight, hydrophobicity, and concentration of nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

Effects and treatment applications of polymeric nanoparticles on improving platelets' storage time: a review of the literature from 2010 to 2020

2021

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Maintaining the quality of platelet products and increasing their storage time are priorities for treatment applications. The formation of platelet storage lesions that limit the storage period and preservation temperature, which can prepare a decent environment for bacterial growth, are the most important challenges that researchers are dealing with in platelet preservation. Nanotechnology is an emerging field of science that has introduced novel solutions to resolve these problems. Here, we reviewed the reported effects of polymeric nanoparticles-including chitosan, dendrimers, polyethylene glycol (PEG), and liposome-on platelets in articles from 2010 to 2020. As a result, we concluded that the presence of dendrimer nanoparticles with a smaller size, negative charge, low molecular weight, and low concentration along with PEGylation can increase the stability and survival of platelets during storage. In addition, PEGylation of platelets can also be a promising approach to improve the quality of platelet bags during storage.

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Influence cationic and anionic PAMAM dendrimers of low generation on selected hemostatic parameters in vitro

Cited by 19 publications

References 34 publications

Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells

Interaction of Cationic Carbosilane Dendrimers and Their siRNA Complexes with MCF-7 Cells

Poly(amidoamine) Dendrimers as Nanocarriers for 5-Fluorouracil: Effectiveness of Complex Formation and Cytotoxicity Studies

Effects and treatment applications of polymeric nanoparticles on improving platelets' storage time: a review of the literature from 2010 to 2020

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