“…Finally, we complemented the experimental work with molecular dynamics (MD) simulations for a better characterization of the nature of PAMAM-5FU interactions at the atomic level. The initial evaluation of cytotoxicity of dendrimers and their complexes with 5FU against mouse fibroblasts (L929), glioblastoma (SNB-19), prostate cancer (Du-145), and colon adenocarcinoma (HT-29) determined via metabolic activity assay of the cells cultured for 7 days in the presence of G4PAMAM dendrimers, G4-5FU complexes, and 5FU, with concentration corresponding to its concentration in complex [15]. The initial evaluation of cytotoxicity of dendrimers and their complexes with 5FU against mouse fibroblasts (L929), glioblastoma (SNB-19), prostate cancer (Du-145), and colon adenocarcinoma (HT-29) determined via metabolic activity assay of the cells cultured for 7 days in the presence of G4PAMAM dendrimers, G4-5FU complexes, and 5FU, with concentration corresponding to its concentration in complex [15].…”