Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis

Arnold, Ludovic; Henry, Adeline; Poron, Françoise; Baba-Amer, Yasmine; Rooijen, Nico van; Plonquet, Anne; Gherardi, Romain K.; Chazaud, Bénédicte

doi:10.1083/jcb1774oia7

Cited by 514 publications

(1,009 citation statements)

References 46 publications

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“…Firstly, in addition to showing significantly less infiltrate than plasmid ET, saline treatment was associated with a more rapid resolution of tissue repair as seen by the switch from pro-to anti-inflammatory macrophages already by day 3, as measured by the number of CD45 þ ve /F4/80 þ ve /Ly6C Àve and CD45 þ ve /F4/80 þ ve /Ly6C þ ve cells, respectively (Supplementary Figures 3b and 4a). 34 Secondly, the pro-inflammatory phase of macrophage infiltration was significantly different between pDCMV and pCpG-free treatments at day 7, but not at day 3. We saw statistically more proinflammatory macrophages in pDCMV compared with pCpG-free plasmid at day 7 by absolute number (74 279.1 ± 49 515 vs 16 024.3±5020 for pDCMV and pCpG-free plasmids, respectively; P ¼ 0.00068) and proportion (52.7 ± 3.0% vs 19.2 ± 0.6%; P ¼ 7.7 Â 10 À6 ), whereas anti-inflammatory macrophages were similar in terms of absolute number (P ¼ 0.086), but statistically different by proportion owing to the reduced total number of CD45 þ ve cells in pCpG-free treated muscle (10.1 ± 2.4% vs 40.8 ± 1.9% for pDCMV and pCpG-free plasmids, respectively; P ¼ 9.7 Â 10 À6 ) (Supplementary Figures 3 and 4 and Supplementary Table 10).…”

Section: Go Biological Process Term Mappingmentioning

confidence: 88%

Molecular signature of the immune and tissue response to non-coding plasmid DNA in skeletal muscle after electrotransfer

et al. 2011

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Electrotransfer of plasmid DNA in skeletal muscle is a common non-viral delivery method for both therapeutic genes and DNA vaccines. Yet, despite the similar approaches, an immune response is detrimental in gene therapy, but desirable for vaccines. However, the full nature of the immune and tissue responses to nucleic acids and electrotransfer in skeletal muscle has not been addressed. Here we used microarray analysis, fluorescence-activated cell sorting and quantitative polymerase chain reaction to obtain the molecular and cellular signature of the tissue and immune response to electrotransfer of saline and non-coding plasmid DNA. Saline electrotransfer resulted in limited infiltration and induction of a moderate damage --repair gene expression pattern not involving innate immune activation. However, plasmid electrotransfer augmented expression of the same genes in addition to inducing a strong innate immune response associated with pro-inflammatory infiltration. In particular, the inflammasome, Toll-like receptor 9 and other pattern recognition receptors able to respond to cytoplasmic DNA were upregulated. Several key differences in the nature of the inflammatory infiltrate and the kinetics of gene expression were also identified when comparing electrotransfer of conventional and CpG-free plasmids. Our data provide insights into the mechanisms of DNA detection and response in muscle that has relevance for non-viral gene therapy and DNA vaccination.

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Section: Go Biological Process Term Mappingmentioning

confidence: 88%

Molecular signature of the immune and tissue response to non-coding plasmid DNA in skeletal muscle after electrotransfer

et al. 2011

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“…They generate inflammatory cytokines and sustain the activation and the proliferation of muscle stem cells. 75 'Alternatively activated' or 'M2' macrophages predominate during the resolution phases of the damage when they regulate the termination of the inflammatory responses. 69,76 Monocyte-derived macrophages undergo dynamic transitions between M1 and M2 and this timely transition is increasingly felt to be the key to muscle homeostasis.…”

Section: The Predators: Macrophages Scavenge Muscle Debrismentioning

confidence: 99%

Cell death, clearance and immunity in the skeletal muscle

et al. 2016

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“…1,20 A number of studies have suggested that infiltrating macrophages along with the trophic factors they release participate in tissue repair of the kidney, 20 -22 brain, 23 skin, 24,25 lung, 26 liver, 27 heart, 28 gastrointestinal tract, 29,30 and skeletal muscle. 31,32 Indeed, the pleiotrophic roles for CSF-1 in reproduction, development of multiple organ systems, and maternal-fetal interactions during pregnancy by macrophage-mediated processes have also been well defined. 2,33,34 To determine the physiological relevance of CSF-1 as a component of the mammalian growth regulatory axis, CSF-1 was administered to neonatal mice.…”

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confidence: 99%

Colony-Stimulating Factor-1 Promotes Kidney Growth and Repair via Alteration of Macrophage Responses

Alikhan

Jones

Williams

et al. 2011

The American Journal of Pathology

131

134

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Colony-stimulating factor (CSF)-1 controls the survival, proliferation, and differentiation of macrophages, which are recognized as scavengers and agents of the innate and the acquired immune systems. Because of their plasticity, macrophages are endowed with many other essential roles during development and tissue homeostasis. We present evidence that CSF-1 plays an important trophic role in postnatal organ growth and kidney repair. Notably, the injection of CSF-1 postnatally enhanced kidney weight and volume and was associated with increased numbers of tissue macrophages. Moreover, CSF-1 promotes postnatal renal repair in mice after ischemia-reperfusion injury by recruiting and influencing macrophages toward a reparative state. CSF-1 treatment rapidly accelerated renal repair with tubular epithelial cell replacement, attenuation of interstitial fibrosis, and functional recovery. Analysis of macrophages from CSF-1-treated kidneys showed increased expression of insulin-like growth factor-1 and anti-inflammatory genes that are known CSF-1 targets. Taken together, these data suggest that CSF-1 is important in kidney growth and the promotion of endogenous repair and resolution of inflammatory injury.

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Inflammatory monocytes recruited after skeletal muscle injury switch into antiinflammatory macrophages to support myogenesis

Cited by 514 publications

References 46 publications

Molecular signature of the immune and tissue response to non-coding plasmid DNA in skeletal muscle after electrotransfer

Molecular signature of the immune and tissue response to non-coding plasmid DNA in skeletal muscle after electrotransfer

Cell death, clearance and immunity in the skeletal muscle

Colony-Stimulating Factor-1 Promotes Kidney Growth and Repair via Alteration of Macrophage Responses

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