Cell death, clearance and immunity in the skeletal muscle

Sciorati, Clara; Rigamonti, Elena; Manfredi, Angelo A.; Rovere‐Querini, Patrizia

doi:10.1038/cdd.2015.171

Cited by 127 publications

(121 citation statements)

References 155 publications

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“…This concept is also supported by the favorable changes of mitochondrial and apoptosis-related gene expression after G1 treatment in OVX rats, which is consistent with previous findings showing that HSPs improve mitochondrial function [19], and protect cells against apoptosis [23]. Apoptosis of skeletal muscle fibers and satellite cells is considered a possible contributor to aging decrements that occur in skeletal muscle [23,24]. …”

Section: Discussionsupporting

confidence: 86%

Activation of GPR30 improves exercise capacity and skeletal muscle strength in senescent female Fischer344 × Brown Norway rats

Wang

Alencar

Lin

et al. 2016

Biochemical and Biophysical Research Communications

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The molecular mechanisms of muscle weakness and sarcopenia in postmenopausal women are largely unknown. To determine the effect of a new estrogen receptor, GPR30, in the maintenance of exercise capacity and skeletal muscle function in females, the selective GPR30 agonist, G1 (100 μg/kg/day), or vehicle (V, soybean oil) was administered subcutaneously daily (n=7 per group) to ovariectomized (OVX) 27-month-old Fischer 344×Brown Norway (F344BN) female rats. Following 8 weeks of treatment, the exercise capacity (treadmill walk time to exhaustion) was reduced in OVX vs. sham rats (5.1±1.4 vs. 11.0±0.9 min, P<0.05), and chronic G1 restored exercise capacity (12.9±1.2 min; P<0.05 vs. OVX-V). Similarly, the peak twitch of electrically stimulated soleus muscles was decreased by 22% in OVX vs. sham rats (P<0.05), and G1 attenuated this decline (P<0.05). Western blot analysis showed that chronic G1 treatment attenuated OVX-associated decreases in heat shock protein (HSP) 90, HSP70, and HSP27 expressions. In vitro studies using the L6 myoblast cell line demonstrated that G1 increased mRNA levels of HSPs in cultured cells. Collectively, these data demonstrate that the activation of GPR30 mitigates the adverse effects of estrogen loss on exercise capacity and skeletal muscle contractile function in old F344BN rats. The protective effects of GPR30 might be through its upregulation of heat shock proteins in skeletal muscle.

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Section: Discussionsupporting

confidence: 86%

Activation of GPR30 improves exercise capacity and skeletal muscle strength in senescent female Fischer344 × Brown Norway rats

Wang

Alencar

Lin

et al. 2016

Biochemical and Biophysical Research Communications

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“…Additionally, it was found that severe muscle injury and cell apoptosis in ducks postmortem have effects on muscle color and water holding capacity in stress state45. Apoptosis often occurs with damage to cytoskeleton proteins and cellular components and shrinkage of muscle cells, which leads to changes of meat quality traits4647.…”

Section: Discussionmentioning

confidence: 99%

Elucidating a molecular mechanism that the deterioration of porcine meat quality responds to increased cortisol based on transcriptome sequencing

Wan

Wang

Xiong

et al. 2016

Sci Rep

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Stress response is tightly linked to meat quality. The current understanding of the intrinsic mechanism of meat deterioration under stress is limited. Here, male piglets were randomly assigned to cortisol and control groups. Our results showed that when serum cortisol level was significantly increased, the meat color at 1 h postmortem, muscle bundle ratio, apoptosis rate, and gene expression levels of calcium channel and cell apoptosis including SERCA1, IP3R1, BAX, Bcl-2, and Caspase-3, were notably increased. However, the value of drip loss at 24 h postmortem and serum CK were significantly decreased. Additionally, a large number of differentially expressed genes (DEGs) in GC regulation mechanism were screened out using transcriptome sequencing technology. A total of 223 DEGs were found, including 80 up-regulated genes and 143 down-regulated genes. A total of 204 genes were enriched in GO terms, and 140 genes annotated into in KEGG database. Numerous genes were primarily involved in defense, inflammatory and wound responses. This study not only identifies important genes and signalling pathways that may affect the meat quality but also offers a reference for breeding and feeding management to provide consumers with better quality pork products.

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“…This, coupled with the macrophage continuum, has led to inconsistencies with regard to identification and nomenclature across fields and across species (Murray et al , 2014). Skeletal muscle macrophages have primarily been studied in rodent models of injury, where the M1 versus M2 macrophage classification has proven useful (Smith et al , 2008; Chazaud et al , 2009; Tidball and Villalta, 2010, Kharraz et al , 2013; Novak and Koh, 2013; Saclier et al , 2013b; Rigamonti et al , 2014; Tidball et al , 2014; Wang et al , 2014; Sciorati et al , 2016; Varga et al , 2016; Mackey and Kjaer, 2017). The skeletal muscle response to injury is characterized by highly orchestrated temporal processes.…”

Section: Introductionmentioning

confidence: 99%

“…The skeletal muscle response to injury is characterized by highly orchestrated temporal processes. Initially, M1 macrophages phagocytize damaged skeletal muscle fibers and debris, followed by M2 macrophage-facilitated repair and regeneration (Chazaud et al , 2009; Tidball and Villalta, 2010; Kharraz et al , 2013; Saclier et al , 2013a; Tidball et al , 2014; Sciorati et al , 2016). Recent work nicely details fiber repair in human skeletal muscle in vivo , showing the presence of macrophages, using a pan-macrophage intracellular marker (CD68+), in regenerating zones along injured fibers (Mackey and Kjaer, 2017).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Identification of Human Skeletal Muscle Macrophages

et al. 2018

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Macrophages have well-characterized roles in skeletal muscle repair and regeneration. Relatively little is known regarding the role of resident macrophages in skeletal muscle homeostasis, extracellular matrix remodeling, growth, metabolism and adaptation to various stimuli including exercise and training. Despite speculation into macrophage contributions during these processes, studies characterizing macrophages in non-injured muscle are limited and methods used to identify macrophages vary. A standardized method for the identification of human resident skeletal muscle macrophages will aide in the characterization of these immune cells and allow for the comparison of results across studies. Here, we present an immunohistochemistry (IHC) protocol, validated by flow cytometry, to distinctly identify resident human skeletal muscle macrophage populations. We show that CD11b and CD206 double IHC effectively identifies macrophages in human skeletal muscle. Furthermore, the majority of macrophages in non-injured human skeletal muscle show a ‘mixed’ M1/M2 phenotype, expressing CD11b, CD14, CD68, CD86 and CD206. A relatively small population of CD11b+/CD206− macrophages are present in resting skeletal muscle. Changes in the relative abundance of this population may reflect important changes in the skeletal muscle environment. CD11b and CD206 IHC in muscle also reveals distinct morphological features of macrophages that may be related to the functional status of these cells.

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Cell death, clearance and immunity in the skeletal muscle

Cited by 127 publications

References 155 publications

Activation of GPR30 improves exercise capacity and skeletal muscle strength in senescent female Fischer344 × Brown Norway rats

Activation of GPR30 improves exercise capacity and skeletal muscle strength in senescent female Fischer344 × Brown Norway rats

Elucidating a molecular mechanism that the deterioration of porcine meat quality responds to increased cortisol based on transcriptome sequencing

Immunohistochemical Identification of Human Skeletal Muscle Macrophages

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