Inflammatory mediators generated at the site of inoculation of Loxosceles gaucho spider venom

Barbaro, Kátia Cristina; Lira, Marcela S.; Araújo, Claudia Andréa Alves de; Pareja-Santos, Alessandra; Távora, Bianca C.L.F.; Prezotto-Neto, José Pedro; Kimura, Louise F.; Lima, Carla; Lopes-Ferreira, Mônica; Santoro, Marcelo Larami

doi:10.1016/j.toxicon.2010.06.022

Cited by 33 publications

(21 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…According to our data, the inflammation increases progressively from two hours and then becomes severe eight hours post L. similis crude venom injection corroborating the already existing studies with L. laeta and L. intermedia venom (15,17,32). Morphometrical parameters showed here can be used to monitor the process dynamics, and also any treatment and sera effectiveness studies (22,31,33,34). In the present study, the heterophils/neutrophils were the predominant cells observed as mentioned by others for loxoscelism.…”

Section: Discussionsupporting

confidence: 91%

“…The average diameter of blood vessels was significantly higher (p < 0.05) in experimental animals compared with the control ones, in all time intervals. These data show that L. similis venom has a vasodilator action, already observed for other Loxosceles, and which is mediated by histamine (30,31). The edema was significantly greater at 2 hours after venom injection.…”

Section: Discussionsupporting

confidence: 75%

See 1 more Smart Citation

Histopathological characterization of experimentally induced cutaneous loxoscelism in rabbits inoculated with Loxosceles similis venom

Pereira

Kalapothakis

Vasconcelos

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Abstract:Envenomation by Loxosceles bites is characterized by dermonecrotic and/or systemic features that lead to several clinical signs and symptoms called loxoscelism. Dermonecrotic lesions are preceded by thrombosis of the dermal plexus. Recent studies show that atheromatous plaque is prone to thrombosis due to endothelial cell apoptosis. To the best of our knowledge, there are no reports of microscopic dermal lesion and endothelial cell apoptosis induced by Loxosceles similis venom in the literature. Thus, the aim of the present study is to describe histological lesions induced by L. similis venom in rabbit skin and to elucidate whether apoptosis of endothelial cells is involved in the pathogenesis of loxoscelism. Forty male rabbits were split into two groups: the control group (intradermally injected with 50 µL of PBS) and the experimental group (intradermally injected with 0.5 µg of L. similis crude venom diluted in 50 µL of PBS). After 2, 4, 6 and 8 hours of injection, skin fragments were collected and processed for paraffin or methacrylate embedding. Sections of 5 µm thick were stained by HE, PAS or submitted to TUNEL reaction. Microscopically, severe edema, diffuse heterophilic inflammatory infiltrate, perivascular heterophilic infiltrate, thrombosis, fibrinoid necrosis of arteriolar wall and cutaneous muscle necrosis were observed. Two hours after venom injection, endothelial cells with apoptosis morphology were evidenced in the dermal plexus. Apoptosis was confirmed by TUNEL reaction. It seems that endothelial cell apoptosis and its consequent desquamation is an important factor that induces thrombosis and culminates in dermonecrosis, which is characteristic of cutaneous loxoscelism.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 75%

Histopathological characterization of experimentally induced cutaneous loxoscelism in rabbits inoculated with Loxosceles similis venom

Pereira

Kalapothakis

Vasconcelos

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…All mouse strains tested here showed a significant edema after venom inoculation. However, it is noteworthy that the paw edema was persistent to the strains C57BL/6 and BALB/c for 24 h. This result was different from that observed for Loxosceles gaucho venom inoculated in the paw of BALB/c mice which persisted for only two hours after inoculation (Barbaro et al, 2010).…”

Section: Discussioncontrasting

confidence: 69%

“…The use of mouse models for the study of loxoscelism is restricted to analysis of inflammatory events, since the dermonecrotic lesion was not observed in mice using intradermal injection of the venom. However, venom toxins promote damage in the liver, kidneys, heart and central nervous system with possible progression to death in these animals (Barbaro et al, 2010;Beckwith et al, 1980;Sunderkotter et al, 2001). The nephrotoxicity of the L. intermedia venom was attributed to disturbances on renal glomeruli and tubules.…”

Section: Introductionmentioning

confidence: 99%

Pattern of inflammatory response to Loxosceles intermedia venom in distinct mouse strains: A key element to understand skin lesions and dermonecrosis by poisoning

Ribeiro

Oliveira

Monteiro-Machado

et al. 2015

Toxicon

View full text Add to dashboard Cite

“…Edema formation has also been observed for nontheraphosid spiders such as P. nigriventer (Antunes et al, 1992Bento et al, 1995;Costa et al, 1997) and Loxosceles spp. (Rattmann et al, 2008;Paludo et al, 2009;Barbaro et al, 2010;Guimarães et al, 2013;Ribeiro et al, 2015), and a variety of Australian spiders (Atkinson, 1986;Korszniak and Story, 1995). Unlike envenoming by Loxosceles spp.…”

Section: Discussionmentioning

confidence: 99%

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Silva

Linardi

Antunes

et al. 2015

Journal of Pharmacology and Experimental Therapeutics

View full text Add to dashboard Cite

Bites by tarantulas (Theraphosidae, Mygalomorphae) in humans can result in mild clinical manifestations such as local pain, erythema, and edema. Vitalius dubius is a medium-sized, nonaggressive theraphosid found in southeastern Brazil. In this work, we investigated the mediators involved in the plasma extravasation caused by V. dubius venom in rats. The venom caused dose-dependent (0.1-100 mg/site) edema in rat dorsal skin. This edema was significantly inhibited by butyl]benzamide) (SR48968, a neurokinin NK 2 receptor antagonist) had no effect on the venom-induced increase in vascular permeability. In rat hind paws, the venom-induced edema was attenuated by ketoprofen (a nonselective COX inhibitor) administered 15 minutes postvenom. Preincubation of venom with commercial antiarachnid antivenom attenuated the venom-induced edema. These results suggest that the enhanced vascular permeability evoked by V. dubius venom involves serotonin, COX products, neurokinin NK 1 receptors, and nitric oxide formation. The attenuation of hind paw edema by ketoprofen suggests that COX inhibitors could be useful in treating the local inflammatory response to bites by these spiders.

show abstract

Inflammatory mediators generated at the site of inoculation of Loxosceles gaucho spider venom

Cited by 33 publications

References 42 publications

Histopathological characterization of experimentally induced cutaneous loxoscelism in rabbits inoculated with Loxosceles similis venom

Histopathological characterization of experimentally induced cutaneous loxoscelism in rabbits inoculated with Loxosceles similis venom

Pattern of inflammatory response to Loxosceles intermedia venom in distinct mouse strains: A key element to understand skin lesions and dermonecrosis by poisoning

Pharmacological Characterization of the Edema Caused by Vitalius dubius (Theraphosidae, Mygalomorphae) Spider Venom in Rats

Contact Info

Product

Resources

About