Inflammatory Granulocytes Decrease Subcutaneous Growth of Melanoma in Mice

Costa, María Teresa; Águas, Artur P.

doi:10.1007/s10753-004-6646-7

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…These cells were chosen based on the H-2 haplotypes, considering that AIRmax mice are predominantly H-2 b , and AIRmin mice are H-2 d [ 6 ]. We then chose S91 melanoma (H-2 d ), originally derived from DBA/2 animals, and B16F10 (H-2 b ), derived from C57BL/6 mice [ 6 , 28 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Activity and Lymphocyte Subtypes in Mice Selected for Maximal and Minimal Inflammatory Response after Transplantation of B16F10 and S91 Melanoma Cells

Castoldi

Romagnoli

Golim

et al. 2022

International Journal of Inflammation

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AIRmax and AIRmin mice strains were selected according to the intensity of their acute inflammatory responsiveness. Previous studies have shown that AIR mice differ in their resistance to chemically induced skin tumors and in the development of melanoma metastases, in addition to differences in neutrophil and NK cells activity. In the present work, we aimed to evaluate whether the difference of susceptibility to murine melanoma is associated with NK cytotoxic activity against Yac.1 cells and lymphocyte subsets. Mice were subcutaneously inoculated with B16F10 or S91 melanoma cells. After 7, 14, or 30 days, the animals were euthanized to analyze the number of lymphocyte subsets, cytotoxic activity, and number of cytokine-producing spleen cells. AIRmax mice presented a higher number of CD4+/CD25+ cells than that of AIRmin mice following inoculation of B16F10 cells, whereas inoculation of S91 cells reduced CD4+/CD25+ and increased TCD8+ cell subsets in the AIRmax mice. AIRmax mice had a higher number of interleukin (IL)-10- and IL-12-producing cells and a lower number of interferon-γ–producing cells than those of AIRmin mice at 30 days. The cytotoxic activity of nonadherent spleen cells was similar in both the AIR strains. These results suggest that melanoma cells can induce different responses in AIR mice, possibly owing to alterations in regulatory mechanisms, such as the action of CD4+/CD25+ regulatory T cells and IL-10, in AIRmax mice.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic Activity and Lymphocyte Subtypes in Mice Selected for Maximal and Minimal Inflammatory Response after Transplantation of B16F10 and S91 Melanoma Cells

Castoldi

Romagnoli

Golim

et al. 2022

International Journal of Inflammation

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Tumour-associated neutrophils in patients with cancer

2019

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The Effects of Lead and Selenium on Melanoma Induction

2015

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Background: Melanoma is a malignant skin cancer and is one of the most aggressive malignancies in humans. Heavy metals, including lead, are known to cause cellular toxicity and have been studied for their potentials to induce apoptosis in tumor cells. Since selenium is considered to act protectively in cases of lead poisoning, this study focused on the effects of sodium selenite and lead chloride, both alone and combined, on melanoma cell apoptosis. Methods: This study was carried out by doing cell culture of melanoma cells (B16-F10 cell line) and using C57BL/6 mice. Melanoma cells suspended in lead (II) chloride, sodium selenite, or lead (II) chloride + sodium selenite solutions were injected subcutaneously to mice to induce tumor growth. After 12 days, tumors were excised and measured, followed by flow cytometry and a statistical analysis using a one-way ANOVA. Results: In the group of mice receiving a single injection of melanoma cells suspended in 10 μmol/l of lead (II) chloride, the growth of tumor was significantly slower than in the control group. In mice treated with lead (II) chloride 50 μmol/l and 100 μmol/l, no tumor was visible at the end of the experiment. With a single injection of lead (II) chloride and sodium selenite at concentrations ≥ 10 μmol/l, the weight and size of the tumor were substantially smaller than in the control group. Conclusion: The effect of lead (II) chloride on melanoma induction is dependent on the concentration of lead (II) chloride. Future applications may include the use of lead (II) chloride to increase apoptosis and necrosis in tumor cells and thus suppress tumor cells proliferation.

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Inflammatory Granulocytes Decrease Subcutaneous Growth of Melanoma in Mice

Cited by 3 publications

References 12 publications

Cytotoxic Activity and Lymphocyte Subtypes in Mice Selected for Maximal and Minimal Inflammatory Response after Transplantation of B16F10 and S91 Melanoma Cells

Cytotoxic Activity and Lymphocyte Subtypes in Mice Selected for Maximal and Minimal Inflammatory Response after Transplantation of B16F10 and S91 Melanoma Cells

Tumour-associated neutrophils in patients with cancer

The Effects of Lead and Selenium on Melanoma Induction

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