Inflammatory and immuno-reactivity in mice induced by intramuscular implants of HSNGLPL peptide grafted-polyurethane

Xiao, Jiangwei; Huang, Cao; Shi, Dandan; Zhu, Rong; Gu, Ruicai; Wang, Huan; Wu, Gang; Liao, Hua

doi:10.1039/c5tb02567b

Cited by 9 publications

(12 citation statements)

References 35 publications

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“…[29][30][31] We previously demonstrated that monocyte/ macrophage responses can be readily induced in the skeletal muscle of B6 mice that received the synthetic implants, 30,31 indicating that the recruitment of these cells into the muscle containing foreign implants is crucial for the subsequent inflammation response and muscle regeneration. Monocytes/macrophages are the most important players in this system.…”

Section: Discussionmentioning

confidence: 99%

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids

Guo

Dong

Xiao

et al. 2018

J Biomedical Materials Res

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Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology. However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined. In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO ) and chitosan-silica-hydroxyapatite (CS/SiO /HA) hybrids, through sol-gel process and 3D plotting skill, followed by the naturally or freeze drying separately. Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids. After intramuscular implantation, CS/SiO and CS/SiO /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration. Naturally dried CS/SiO hybrid provoked a more severe inflammation than the freeze-dried ones. Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants. Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation. Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease. Thus, the present results suggest that, in vivo, 3D plotted porous CS/SiO and CS/SiO /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1223-1235, 2018.

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Section: Discussionmentioning

confidence: 99%

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids

Guo

Dong

Xiao

et al. 2018

J Biomedical Materials Res

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“…The degrading implants can cause myofiber degeneration that can be detected by H&E staining. 63 We did not notice any myofiber necrosis in the muscle tissue surrounding our samples, indicating that AS coating is nontoxic and does not cause a foreign body reaction. Structure of the myofibers was normal with well-defined nuclei at the implant interface as well as in the deeper layers of the muscle.…”

Section: Discussionmentioning

confidence: 51%

In vivo study of self‐assembled alkylsilane coated degradable magnesium devices

et al. 2018

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Magnesium (Mg) and its alloys are candidate materials for resorbable implantable devices, such as orthopedic devices or cardiovascular stents. Mg has a number advantages, including mechanical properties, light weight, its osteogenic effects and the fact that its degradation products are nontoxic and naturally present in the body. However, production of H gas during the corrosion reaction can cause formation of gas pockets at the implantation site, posing a barrier to clinical applications of Mg. It is therefore desirable to develop methods to control corrosion rate and gas pocket formation around the implants. Here we evaluate the potential of self-assembled multilayer alkylsilane (AS) coatings to control Mg device corrosion and formation of gas pockets in vivo and to assess effects of the AS coatings on the surrounding tissues in a subcutaneous mouse model over a 6 weeks' period. The coating significantly slowed down corrosion and gas pocket formation as evidenced by smaller gas pockets around the AS coated implants (ANOVA; p = 0.013) and decrease in the weight loss values (t test; p = 0.07). Importantly, the microCT and profilometry analyses demonstrated that the coating inhibited the pitting corrosion. Specifically, the roughness of the coated samples was ∼30% lower than uncoated specimen (p = 0.02). Histological assessment of the tissues under the implant revealed no inflammation or foreign body reaction. Overall, our results demonstrate the feasibility of use of the seld assembled AS coatings for reduction of gas pocket formation around the resorbable Mg devices. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018.

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“…The N 1s peak intensity significantly increased after TGF‐β conjugated onto PCDU‐Peptide surface. Our previous report showed the N 1s peak intensity increases after the peptides were grafted onto the PU as pendent groups, since the nitrogen relative amounts in the peptide are higher than that in polyurethane . The N 1s peak intensity increase in Figure may also be attributed to the TGF‐β conjugated on PCDU‐peptide surface, because the factor has higher compositional N elements in its molecule than that of PCDU‐peptide.…”

Section: Resultsmentioning

confidence: 86%

“…In our previous work, we have reported the synthesis of PU with the pendent HSNGLPL peptide groups and the presenting of the characteristic absorption bands of the polymer by FTIR, XPS, and 1 H‐NMR spectra . Here, we further compared the XPS spectra of PCDU‐Peptide film with and without conjugating TGF‐β showing elements changes after the growth factor's affinity binding.…”

Section: Resultsmentioning

confidence: 94%

“…We had developed a PU clicked with pendent HSNGLPL group, which have strong affinity to the transforming growth factor‐betas (TGF‐β). The reaction procedure of the PU material was monitored and characterized by FTIR, NMR, and X‐ray photoelectron spectroscopy (XPS) to prove the proper structure . Moreover, the bio‐ and immuno‐reactivity in vivo for this peptide‐grafted PUs had been carefully investigated …”

Section: Introductionmentioning

confidence: 99%

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Polyurethane conjugating TGF‐β on surface impacts local inflammation and endoplasmic reticulum stress in skeletal muscle

Shi

Xiao

et al. 2017

J Biomedical Materials Res

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The synthesized short peptide-polymers would provide key functions for tissue regeneration and repair, through enriching bioactive molecules on polymers or releasing these molecules pre-conjugated on the materials. We have developed a degradable polyurethane (PU) bearing HSNGLPL peptide, which has affinity binding ability to transforming growth factor-betas (TGF-β). For deeply understanding spatial release of TGF-β from the PU polymers and its localized bioactivity, quartz crystal microbalance (QCM) and Elisa test were used to verify TGF-β binding capacities in vitro and in vivo. The PU polymers, with or without pre-conjugating of TGF-β, were implanted into gastronomies muscle (GN) of C57BL/6 mice, for addressing TGF-β release from the polymers and its bio-regulating function in vivo. QCM result shows that PU bearing HSNGLPL peptide has affinity binding ability to TGF-β in vitro. Intramuscular implanting experiment further supports the enrichment efficiency of TGF-β on PU polymers in vivo. The detecting data involving intramuscular inflammatory infiltration triggered by the implants, myofiber regeneration, muscular fibrosis degree, and activation of endoplasmic reticulum stress (ER stress), evidence TGF-β can be released from PU polymers, and exerts regulating effects on the material-induced inflammation. Thus, our present results suggest it is feasible to improve biocompatibility of PU polymers in vivo, by pre-bearing bioactive molecules on materials before the implanting. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1156-1165, 2017.

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Inflammatory and immuno-reactivity in mice induced by intramuscular implants of HSNGLPL peptide grafted-polyurethane

Abstract: Synthetic peptide-based polyurethanes (PUs), introduced as bioactive agents and possessing impressive properties, have emerged as attractive functional biomaterials for tissue regeneration.

Cited by 9 publications

References 35 publications

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids

In vivo study of self‐assembled alkylsilane coated degradable magnesium devices

Polyurethane conjugating TGF‐β on surface impacts local inflammation and endoplasmic reticulum stress in skeletal muscle

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Inflammatory and immuno-reactivity in mice induced by intramuscular implants of HSNGLPL peptide grafted-polyurethane

Abstract: Synthetic peptide-based polyurethanes (PUs), introduced as bioactive agents and possessing impressive properties, have emerged as attractive functional biomaterials for tissue regeneration.

Cited by 9 publications

References 35 publications

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO2 and chitosan/SiO2/hydroxyapatite hybrids

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO2 and chitosan/SiO2/hydroxyapatite hybrids

In vivo study of self‐assembled alkylsilane coated degradable magnesium devices

Polyurethane conjugating TGF‐β on surface impacts local inflammation and endoplasmic reticulum stress in skeletal muscle

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Product

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In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids

In vivo immuno‐reactivity analysis of the porous three‐dimensional chitosan/SiO₂ and chitosan/SiO₂/hydroxyapatite hybrids