Inflammation in Wound Repair: Role and Function of Inflammation in Wound Repair

MacLeod, Amanda S.; Kwock, J.

doi:10.1002/9781119282518.ch14

Cited by 5 publications

(4 citation statements)

References 144 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The etiology of chronic wounds is multifaceted, involving factors like overproduction of proinflammatory cytokines, oxidative stress, cellular senescence, infections and impaired stem cell function [6,7]. Persistent inflammation, a pivotal aspect of wound healing, impedes proper healing, resulting in chronic wounds [8]. Transforming growth factor-β (TGF-β), a crucial cytokine in wound healing, has a dual role, initially facilitating healing and potentially contributing to chronic wound pathogenesis when its activity is prolonged [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Enhancing chronic wound healing with Thai indigenous rice variety, Kaab Dum: Exploring ER stress and senescence inhibition in HaCaT keratinocyte cell line

Payuhakrit,

Panpinyaporn,

Khumsri

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Kaab Dum, a prominent indigenous rice variety cultivated in the Pak Phanang Basin of Nakhon Si Thammarat, Thailand, is the focus of our study. We investigate the therapeutic potential of indigenous Kaab Dum rice extract in the context of chronic wounds. Our research encompasses an examination of the nutritional compositions and chemical profiles of Kaab Dum rice extract. Additionally, we assess how the extract affects chronic wounds in TGF-β-induced HaCaT cells. Our evaluation methods include the detection of cellular oxidative stress, the examination of endoplasmic reticulum (ER) stress, wound healing assays, analysis of cell cycle arrest and the study of cellular senescence through senescence-associated β-galactosidase (SA-β-gal) staining. Our research findings demonstrate that TGF-β induces oxidative stress in HaCaT cells, which subsequently triggers ER stress, confirmed by the expression of the PERK protein. This ER stress results in cell cycle arrest in HaCaT cells, characterized by an increase in p21 protein, a cyclin-dependent kinase inhibitor (CDKI). Ultimately, this leads to cellular senescence, as confirmed by SA-β-gal staining. Importantly, our study reveals the effectiveness of Kaab Dum rice extract in promoting wound healing in the chronic wound model. The extract reduces ER stress and senescent cells. These beneficial effects are potentially linked to the antioxidant and anti-inflammatory properties of the rice extract. The findings of our study have the potential to make significant contributions to the development of enhanced products for both the prevention and treatment of chronic wounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Enhancing chronic wound healing with Thai indigenous rice variety, Kaab Dum: Exploring ER stress and senescence inhibition in HaCaT keratinocyte cell line

Payuhakrit,

Panpinyaporn,

Khumsri

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…The skin is the interface between body and environmental factors, and it acts as the first line of defense against many insults, including microbes. 36,65 It plays a fundamental role in innate antimicrobial host immunity. 66 Moreover, with the aging process of the skin, the three layers of skin (epidermis, dermis, and hypodermis) undergo degenerative changes, and alterations of the dermis are the most obvious.…”

Section: Discussionmentioning

confidence: 99%

Relation Between Reactive Oxygen Species Production and Transient Receptor Potential Vanilloid1 Expression in Human Skin During Aging

Favero,

Gianò,

Franco

et al. 2024

J Histochem Cytochem.

View full text Add to dashboard Cite

Skin sensitivity and impaired epidermal barrier function are associated with aging and are at least partly due to increased production of reactive oxygen species (ROS). Transient receptor potential vanilloid1 (TRPV1) is expressed in keratinocytes, fibroblasts, mast cells, and endothelial cells in skin. We investigated in skin biopsies of adult and elderly donors whether TRPV1 expression is involved in the skin aging process. We found that aging skin showed a strongly reduced epidermal thickness, strongly increased oxidative stress, protease expression, and mast cell degranulation and strongly increased TRPV1 expression both in epidermis and dermis. Based on our findings, the aging-related changes observed in the epidermis of the skin level are associated with increased ROS production, and hypothesized alterations in TRPV1 expression are mechanistically linked to this process.

show abstract

“…The most important step of the healing process for wounds is the inflammatory phase. Prolonged inflammation, on the other hand, causes increased cytokine production, such as IL-1, IL-6, and TNF-, severe healing disturbances, and increasing fibrosis and scarring [ 60 ]. TNF-α is a proinflammatory cytokine expressed in higher levels during the inflammatory stages than at other times [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo Investigation

El-Sayed,

Morad,

Sonbol

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Bacterial-associated wound infections are an obstacle for individuals and the medical industry. Developing versatile, antibiotic-free therapies helps heal wounds more quickly and efficiently. In the current study, fungal metabolites were employed as a reducing agent in fabricating selenium nanoparticles (SeNPs) for improved antibacterial and wound healing properties. Utilizing UV-visible spectroscopy, dynamic light scattering (DLS), zeta potential, X-ray diffraction (XRD), and electron microscopic examination, the properties of the synthesized nanoparticles were extensively evaluated. Myco-synthesized SeNPs demonstrated strong antibacterial activity against Staphylococcus aureus ATCC 6538 with a minimum inhibitory concentration of 0.3125 mg/mL, reducing cell number and shape distortion in scanning electron microscope (SEM) images. SeNPs’ topical administration significantly reduced wound area and healing time, exhibiting the least bacterial load after six days compared to controls. After six and 11 days of treatment, SeNPs could decrease proinflammatory cytokines IL-6 and TNF-α production. The histopathological investigation showed a healed ulcer with moderate infiltration of inflammatory cells after exposing mice’s skin to SeNPs for six and 11 days. The docking interaction indicated that SeNPs were highly efficient against the IL-6 and TNF-α binding receptors. These findings imply that myco-fabricated SeNPs might be used as topically applied antimicrobial agents for treating skin infections and wounds.

show abstract

Inflammation in Wound Repair: Role and Function of Inflammation in Wound Repair

Cited by 5 publications

References 144 publications

Enhancing chronic wound healing with Thai indigenous rice variety, Kaab Dum: Exploring ER stress and senescence inhibition in HaCaT keratinocyte cell line

Enhancing chronic wound healing with Thai indigenous rice variety, Kaab Dum: Exploring ER stress and senescence inhibition in HaCaT keratinocyte cell line

Relation Between Reactive Oxygen Species Production and Transient Receptor Potential Vanilloid1 Expression in Human Skin During Aging

Myco-Synthesized Selenium Nanoparticles as Wound Healing and Antibacterial Agent: An In Vitro and In Vivo Investigation

Contact Info

Product

Resources

About