Infiltration of trace metal ions in the oral mucosa of a rat analyzed using SRXRF, XAFS, and ICP-MS

Abstract

Although the accumulation and distribution of metals from metallic orthodontic appliances in the oral mucosa have been studied extensively, they remain unclear because their concentration is quite low. In this study, metal specimens (Ni, Ni-Ti, and Co-Cr) were sutured in the unilateral oral mucosa of rats, and the distribution of the eluted elements in the mucosal tissue was estimated using inductively coupled plasma mass spectrometry (ICP-MS) and synchrotron radiation X-ray fluorescence analysis (SR-XRF). While the infiltrations of Ni, Co, and Cr into the oral mucosal connective tissue were observed with SR-XRF, significant increases were only found in Ni from the pure Ni group and Cr from the Co-Cr group. Furthermore, Ni and Co were estimated as hydrated ions while Cr was estimated in oxide form through X-ray absorption fine structure (XAFS) analysis.

Suprabasin-null mice retain skin barrier function and show high contact hypersensitivity to nickel upon oral nickel loading

“…In the oral mucosa, esophagus, and skin of Sbsn -/mice, there was no inflammation in a steady state. In addition to duodenum, upper digestive tract is an important site for the absorption of metals [18][19][20] . In conventional housing of mice, the blood nickel concentration was under detection level in both WT and Sbsn -/mice.…”

“…In addition to duodenum, upper digestive tract is an important site for the absorption of metals 18 – 20 . In conventional housing of mice, the blood nickel concentration was under detection level in both WT and Sbsn –/– mice.…”

Comparative analysis of the surface properties and corrosion resistance of Co-Cr dental alloys fabricated by different methods

“…38 The qualitative and quantitative analyses of the released elements are regarded as the most effective measure of the corrosion behavior and biological effect of metallic biomaterial. 19 Although Co-Cr dental alloy shows acceptable corrosion resistance and allergic reactions are rare compared with Ni-Cr alloys, 39 Co 2+ has been reported to have a cytotoxic effect on the human monocyte cell line or induce macrophage retention at inflammatory sites. 40,41 Furthermore, Shettlemore and Bundy 42 reported the toxicity rank order (Cr 6+ >Co 2+ >Cr 3+ ) by assessing corrosion products produced by the Co-Cr alloy.…”

“…The changeable oral environment, including temperature, pH, and pressure, has been reported to accelerate corrosion and the release of metal ions, which may induce deoxyribonucleic acid (DNA) damage and cell apoptosis. [18][19][20] Furthermore, corrosion resistance has been strongly associated with the microstructures and chemical composition of the alloy. 20 Therefore, the purpose of this in vitro study was to investigate the surface and corrosion properties of Co-Cr dental alloys fabricated by different methods (CAST, SLM, and CNC).…”

Toxicological aspects of soluble titanium – a review of in vitro and in vivo studies

“…Several reports focused on the release of titanium into periimplant tissues. 58,61,64,66 Metal does not seem to accumulate in the mucosal tissue, irrespective of whether following a short (2 weeks) or long (12 months) implantation time. 58,66 However, an increased Ti level was observed in the gingiva around a titanium mini-implant inserted in the palatal process of rats for seven days.…”