Infectivity of<i>Plasmodium berghei</i>Sporozoites Delivered by Intravenous Inoculation versus Mosquito Bite: Implications for Sporozoite Vaccine Trials

Vaughan, Jefferson A.; Scheller, Libia F.; Wirtz, Robert A.; Azad, Abdu F.

doi:10.1128/iai.67.8.4285-4289.1999

Cited by 67 publications

(23 citation statements)

References 54 publications

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“…Humoral immune responses against mosquito saliva have been frequently observed using ICR mice (Limesand et al, 2000;Ohtsuka et al, 2001;Thangamani et al, 2010). Moreover, ICR mice were used for the study of mosquito bite P. berghei challenge model (Vaughan et al, 1999). DsRed-PbCS3R transgenic or WT female mosquitoes at days 7-10 after emergence were allowed to feed on blood from all over the body of individual mice.…”

Section: Immunization Of Mice With Tg Mosquito Bitesmentioning

confidence: 99%

Induction of antisporozoite antibodies by biting of transgenic Anopheles stephensi delivering malarial antigen via blood feeding

Yamamoto

Sumitani

Nagumo

et al. 2012

Insect Molecular Biology

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We produced a transgenic mosquito expressing a rodent malaria vaccine candidate antigen in the salivary gland. Three tandemly repeated amino acid units from the repeat region of circumsporozoite protein of Plasmodium berghei (PbCS3R) fused to red fluorescent protein (monomeric DsRed) was chosen as a vaccine candidate antigen. Immunoblot and fluorescence microscopic analyses showed the transgene expression in the female salivary gland. The transgene product was released from the proboscis as a component of saliva. The monomeric DsRed-fusion expression system could be suitable for transgene secretion in the saliva of female mosquitoes. Mice repeatedly bitten by transgenic mosquitoes raised antibodies against P. berghei sporozoites, and the sera had protective ability against sporozoite invasion of human hepatoma HepG2 cells. These results suggest that transgene products are immunogenically active in saliva, and induce the antibodies to malaria parasite. These findings indicate that this technology has the potential for production of a 'flying vaccinator' for rodent malaria parasites.

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Section: Immunization Of Mice With Tg Mosquito Bitesmentioning

confidence: 99%

Induction of antisporozoite antibodies by biting of transgenic Anopheles stephensi delivering malarial antigen via blood feeding

Yamamoto

Sumitani

Nagumo

et al. 2012

Insect Molecular Biology

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“…Bites from infected mosquitoes or sporozoite injection can be used for experimental sporozoite inoculation. Sporozoites isolated from salivary glands of mosquitoes have a limited ex vivo viability [136,137], and methods to improve their preservation would be useful.…”

Section: Tools Required Facilitating In Vivo Imaging Of Malaria In Nhmentioning

confidence: 99%

In vivo imaging in NHP models of malaria: Challenges, progress and outlooks

Beignon

Grand

Chapon

2014

Parasitology International

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Animal models of malaria, mainly mice, have made a large contribution to our knowledge of host-pathogen interactions and immune responses, and to drug and vaccine design. Non-human primate (NHP) models for malaria are admittedly under-used, although they are probably closer models than mice for human malaria; in particular, NHP models allow the use of human pathogens (Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium knowlesi). NHPs, whether natural hosts or experimentally challenged with a simian Plasmodium, can also serve as robust pre-clinical models. Some simian parasites are closely related to a human counterpart, with which they may share a common ancestor, and display similar major features with the human infection and pathology. NHP models allow longitudinal studies, from the early events following sporozoite inoculation to the later events, including analysis of organs and tissues, particularly liver, spleen, brain and bone marrow. NHP models have one other significant advantage over mouse models: NHPs are our closest relatives and thus their biology is very similar to ours. Recently developed in vivo imaging tools have provided insight into malaria parasite infection and disease in mouse models. One advantage of these tools is that they limit the need for invasive procedures, such as tissue biopsies. Many such technologies are now available for NHP studies and provide new opportunities for elucidating host/parasite interactions. The aim of this review is to bring the malaria community up to date on what is currently possible and what soon will be, in terms of in vivo imaging in NHP models of malaria, to consider the pros and the cons of the various techniques, and to identify challenges.

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“…Plasmodium sporozoites provide an interesting case study for highlighting the difference of the syringe versus natural injection approaches, albeit not all aspects are understood. Clearly, even when injected by syringe intravenously, sporozoites are not as infectious as when injected by natural bite (Vaughan et al ., 1999). Similar differences were shown in avian malaria when sporozoites were injected intradermally (Krettli and Dantas, 2000).…”

Section: Modulation Of Natural Infection By Insect Salivamentioning

confidence: 99%

The skin as interface in the transmission of arthropod-borne pathogens

Frischknecht¹

2007

Cell Microbiol

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SummaryAnimal skin separates the inner world of the body from the largely hostile outside world and is actively involved in the defence against microbes. However, the skin is no perfect defence barrier and many microorganisms have managed to live on or within the skin as harmless passengers or as disease-causing pathogens. Microbes have evolved numerous strategies that allow them to gain access to the layers underneath the epidermis where they either multiply within the dermis or move to distant destinations within the body for replication. A number of viruses, bacteria and parasites use arthropod vectors, like ticks or mosquitoes, to deliver them into the dermis while taking their blood meal. Within the dermis, successful pathogens subvert the function of a variety of skin resident cells or cells of the innate immune system that rush to the site of infection. In this review several interactions with cells of the skin by medically relevant vector-borne pathogens are discussed to highlight the different ways in which these pathogens have come to survive within the skin and to usurp the defence mechanisms of the host for their own ends.

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Infectivity ofPlasmodium bergheiSporozoites Delivered by Intravenous Inoculation versus Mosquito Bite: Implications for Sporozoite Vaccine Trials

Cited by 67 publications

References 54 publications

Induction of antisporozoite antibodies by biting of transgenic Anopheles stephensi delivering malarial antigen via blood feeding

Induction of antisporozoite antibodies by biting of transgenic Anopheles stephensi delivering malarial antigen via blood feeding

In vivo imaging in NHP models of malaria: Challenges, progress and outlooks

The skin as interface in the transmission of arthropod-borne pathogens

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