Induction of antisporozoite antibodies by biting of transgenic <i>Anopheles stephensi</i> delivering malarial antigen via blood feeding

Yamamoto, Daisuke; Sumitani, Megumi; Nagumo, H.; Yoshida, Shigeto; Matsuoka, Hiroyuki

doi:10.1111/j.1365-2583.2011.01128.x

Cited by 16 publications

(27 citation statements)

References 47 publications

(60 reference statements)

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“…These results are consistent with our previous findings showing the expression of the non-toxic fluorescent protein DsRed. In these transgenic lines, strong red fluorescence was observed in the distal-lateral lobes [13–15]. …”

Section: Resultsmentioning

confidence: 99%

“…stephensi aapp promoter (pAAPP) and An . gambiae trypsin terminator (tryter) were amplified by PCR from pMinos-EGFP-aappP-mDsRed-Pbcsp-antryp1T [15] by the primers pAAPP-SalI: 5’-GCGTCGACCACCTTATAAGACGGAGCTC-3’ and pAAPPR-XbaI: 5’-GCTCTAGACGTTTATTCACCTGTGAACTG-3’, TrypolyA-NotI: 5’-GCGGCCGCATGCGATCTCGGCTTCAAAAC-3’ and TrypolyA-SpeI: 5’-ACTAGTCACCCTTCAGCGAAAGCTTGTC-3’, respectively. PCR reactions were performed using KOD plus neo polymerase (Toyobo, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

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Inhibition of Malaria Infection in Transgenic Anopheline Mosquitoes Lacking Salivary Gland Cells

et al. 2016

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Malaria is an important global public health challenge, and is transmitted by anopheline mosquitoes during blood feeding. Mosquito vector control is one of the most effective methods to control malaria, and population replacement with genetically engineered mosquitoes to block its transmission is expected to become a new vector control strategy. The salivary glands are an effective target tissue for the expression of molecules that kill or inactivate malaria parasites. Moreover, salivary gland cells express a large number of molecules that facilitate blood feeding and parasite transmission to hosts. In the present study, we adapted a functional deficiency system in specific tissues by inducing cell death using the mouse Bcl-2-associated X protein (Bax) to the Asian malaria vector mosquito, Anopheles stephensi. We applied this technique to salivary gland cells, and produced a transgenic strain containing extremely low amounts of saliva. Although probing times for feeding on mice were longer in transgenic mosquitoes than in wild-type mosquitoes, transgenic mosquitoes still successfully ingested blood. Transgenic mosquitoes also exhibited a significant reduction in oocyst formation in the midgut in a rodent malaria model. These results indicate that mosquito saliva plays an important role in malaria infection in the midgut of anopheline mosquitoes. The dysfunction in the salivary glands enabled the inhibition of malaria transmission from hosts to mosquito midguts. Therefore, salivary components have potential in the development of new drugs or genetically engineered mosquitoes for malaria control.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Inhibition of Malaria Infection in Transgenic Anopheline Mosquitoes Lacking Salivary Gland Cells

et al. 2016

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“…The Anopheles stephensi Swiss 500 strain was reared in our laboratory [9]. BALB/c mice were purchased from SRL Japan (Shizuoka) and reared at 26°C with 60–70% relative humidity under a 13 h-light/11 h-dark cycle.…”

Section: Methodsmentioning

confidence: 99%

Visualization of Malaria Parasites in the Skin Using the Luciferase Transgenic Parasite,<i> Plasmodium berghei</i>

et al. 2015

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We produced a transgenic rodent malaria parasite (Plasmodium berghei) that contained the luciferase gene under a promoter region of elongation factor-1α. These transgenic (TG) parasites expressed luciferase in all stages of their life cycle, as previously reported. However, we were the first to succeed in observing sporozoites as a mass in mouse skin following their deposition by the probing of infective mosquitoes. Our transgenic parasites may have emitted stronger bioluminescence than previous TG parasites. The estimated number of injected sporozoites by mosquitoes was between 34 and 775 (median 80). Since luciferase activity diminished immediately after the death of the parasites, luciferase activity could be an indicator of the existence of live parasites. Our results indicated that sporozoites survived at the probed site for more than 42 hours. We also detected sporozoites in the liver within 15 min of the intravenous injection. Bioluminescence was not observed in the lung, kidney or spleen. We confirmed the observation that the liver was the first organ in which malaria parasites entered and increased in number.

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“…The A. stephensi mosquito strain SDA500 was maintained at Jichi Medical University according to previously described methods (Yamamoto et al, 2012). Female ICR strain mice were obtained from Japan SLC (Hamamatsu, Shizuoka, Japan) and were used at 8weeks of age.…”

Section: Animalsmentioning

confidence: 99%

“…Several effector molecules against the malaria parasite have been expressed in the midgut, hemolymph and salivary glands of mosquitoes (Ito et al, 2002;Kokoza et al, 2010;Dong et al, 2011;Isaacs et al, 2011;Isaacs et al, 2012;Sumitani et al, 2013). Moreover, vaccine candidate molecules have been shown to be expressed in salivary glands (Matsuoka et al, 2010;Yamamoto et al, 2010;Yamamoto et al, 2012). The stable maintenance of a large number of transgenic lines requires a large effort and cost.…”

Section: Introductionmentioning

confidence: 99%

Artificial activation of mature unfertilized eggs in the malaria vector mosquito, Anopheles stephensi (Diptera, Culicidae)

Yamamoto

Hatakeyama

Matsuoka

2013

Journal of Experimental Biology

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SUMMARYIn the past decade, many transgenic lines of mosquitoes have been generated and analyzed, whereas the maintenance of a large number of transgenic lines requires a great deal of effort and cost. In vitro fertilization by an injection of cryopreserved sperm into eggs has been proven to be effective for the maintenance of strains in mammals. The technique of artificial egg activation is a prerequisite for the establishment of in vitro fertilization by sperm injection. We demonstrated that artificial egg activation is feasible in the malaria vector mosquito, Anopheles stephensi (Diptera, Culicidae). Nearly 100% of eggs dissected from virgin females immersed in distilled water darkened, similar to normally oviposited fertilized eggs. It was revealed by the cytological examination of chromosomes that meiotic arrest was relieved in these eggs approximately 20min after incubation in water. Biochemical examinations revealed that MAPK (mitogen-activated protein kinase)/ERK (extracellular signal-regulated protein kinase) and MEK (MAPK/ERK kinase) were dephosphorylated similar to that in fertilized eggs. These results indicate that dissected unfertilized eggs were activated in distilled water and started development. Injection of distilled water into body cavity of the virgin blood-fed females also induced activation of a portion of eggs in the ovaries. The technique of artificial egg activation is expected to contribute to the success of in vitro fertilization in A. stephensi.

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Induction of antisporozoite antibodies by biting of transgenic Anopheles stephensi delivering malarial antigen via blood feeding

Cited by 16 publications

References 47 publications

Inhibition of Malaria Infection in Transgenic Anopheline Mosquitoes Lacking Salivary Gland Cells

Inhibition of Malaria Infection in Transgenic Anopheline Mosquitoes Lacking Salivary Gland Cells

Visualization of Malaria Parasites in the Skin Using the Luciferase Transgenic Parasite,<i> Plasmodium berghei</i>

Artificial activation of mature unfertilized eggs in the malaria vector mosquito, Anopheles stephensi (Diptera, Culicidae)

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