Infectious Bronchitis Virus S2 Expressed from Recombinant Virus Confers Broad Protection Against Challenge

Toro, Haroldo; Zhao, Wei; Breedlove, C.; Zhang, Zhenyu; Santen, Vicky L. van; Yu, Qingzhong

doi:10.1637/10641-081613-reg.1

Cited by 43 publications

(32 citation statements)

References 48 publications

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“…With a prime-boost application, 42-day-old SPF birds survived a virulent NDV challenge two weeks after the last vaccine was administered (Kanabagatte Basavarajappa et al, 2014). During the same year, recombinant LaSota virus containing the IBV S2 gene was also generated as a priming vaccine (Toro et al, 2014).…”

Section: Ndv-vectored Vaccinesmentioning

confidence: 99%

Newcastle disease vaccines—A solved problem or a continuous challenge?

Dimitrov

Afonso

et al. 2017

Veterinary Microbiology

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Newcastle disease (ND) has been defined by the World Organisation for Animal Health as infection of poultry with virulent strains of Newcastle disease virus (NDV). Lesions affecting the neurological, gastrointestinal, respiratory, and reproductive systems are most often observed. The control of ND must include strict biosecurity that prevents virulent NDV from contacting poultry, and also proper administration of efficacious vaccines. When administered correctly to healthy birds, ND vaccines formulated with NDV of low virulence or viral-vectored vaccines that express the NDV fusion protein are able to prevent clinical disease and mortality in chickens upon infection with virulent NDV. Live and inactivated vaccines have been widely used since the 1950's. Recombinant and antigenically matched vaccines have been adopted recently in some countries, and many other vaccine approaches have been only evaluated experimentally. Despite decades of research and development towards formulation of an optimal ND vaccine, improvements are still needed. Impediments to prevent outbreaks include uneven vaccine application when using mass administration techniques in larger commercial settings, the difficulties associated with vaccinating free-roaming, multi-age birds of village flocks, and difficulties maintaining the cold chain to preserve the thermo-labile antigens in the vaccines. Incomplete or improper immunization often results in the disease and death of poultry after infection with virulent NDV. Another cause of decreased vaccine efficacy is the existence of antibodies (including maternal) in birds, which can neutralize the vaccine and thereby reduce the effectiveness of ND vaccines. In this review, a historical perspective, summary of the current situation for ND and NDV strains, and a review of traditional and experimental ND vaccines are presented.

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Section: Ndv-vectored Vaccinesmentioning

confidence: 99%

Newcastle disease vaccines—A solved problem or a continuous challenge?

Dimitrov

Afonso

et al. 2017

Veterinary Microbiology

Self Cite

283

230

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“…S1 is a major protective antigen for IBV, but is highly variable among different IBV stains (Ignjatovic and Galli, 1994). In contrast to S1, S2 is highly conserved and carries broad antigenic epitopes (Toro et al, 2014). Therefore, S2 could be a marker for developing more efficient vaccines and broad diagnostic approaches.…”

Section: Introductionmentioning

confidence: 99%

“…(Ignjatovic and Galli, 1995) found that S2 glycoprotein was the most immunogenic structural protein following vaccination with inactivated virus. S2 protein expressed from recombinant virus confers broad protection against challenge (Toro et al, 2014). Therefore, S2 protein has important roles in inducing protective immunity (Eldemery et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Peptides with 16R in S2 protein showed broad reactions with sera against different types of infectious bronchitis viruses

Lin

Qian

et al. 2019

Veterinary Microbiology

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Vaccination plays a vital role in controlling diseases caused by chicken infectious bronchitis virus (IBV). The continuously variant antigenicity of IBV limits the application of current vaccine strategies and serological diagnostic systems. S2 protein is an invariant that harbors broad neutralizing epitopes. However, little is known about the key amino acids that contribute to the broad-spectrum S2 epitopes. In this study, we aimed to elucidate the specific amino acids contributing to S2 epitopes. Site mutagenesis and peptide-based enzyme-linked immunosorbent assays (ELISAs) showed that 16R in S2 protein was a key amino acid mediating the antigenicity of S2 protein. S2-derived peptides with 16R, but not those with 16 K, could react with sera against different types of IBVs. Notably, a commercial ELISA kit for detection of antibodies against IBV did not react with sera against all types of IBVs. Taken together, these data demonstrated that S2-derived peptides with 16R could be used as novel marker-based antigens for developing both broad-spectrum vaccines and serological diagnostic kits to control IBV.

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“…A number of foreign antigens derived from avian viral pathogens, such as the haemagglutinin (HA) gene of avian influenza virus, the VP2 gene of infectious bursal disease virus, the S2 gene of infectious bronchitis virus, the glycoprotein B (gB) and D (gD) of infectious laryngotracheitis virus, and the glycoprotein (G) gene of avian metapneumovirus (DiNapoli et al, 2010;Hu et al, 2011;Huang et al, 2004;Toro et al, 2014;Zhao et al, 2014), have been expressed by NDV as bivalent vaccines against NDV and the target avian pathogen challenges. However, most of the NDV vaccine vector strains are thermolabile and may not be suitable for use as vaccine vectors for village chickens.…”

Section: Introductionmentioning

confidence: 99%