Infection status and molecular identification of digenean cercariae in snails in Kim Son district, Ninh Binh Province and Ba Vi district, Ha Noi

Abstract

In this study, the molecular method was used to identify digenean cercariae from freshwater snails in Kim Son District (Ninh Binh Province) and Ba Vi District Ha Noi. A total of 9 snail species were collected and examined for cercarial infection. Three snail species (Radix swinhoei, Angulyagra polyzonata and Pomacea canaliculata) were not infected, while the other 6 species (Austropeplea viridis, Gyraulus convexiusculus, Parafossarulus striatulus, Bithynia fuchsiana, Melanoides tuberculata and Tarebia granifera) were infected with digenean cercariae at low infection rates, ranging from 2.3% to 6.3%. Seven cercarial groups were identified: Echinostome, Monostome, Parapleurophocercaria, Xiphidiocercaria, Fucocercaria, Gymnocephalous and Megalurous. Snails M. tuberculata and P. striatulus were infected with 4 cercarial groups, A. viridis and G. convexiusculus snails were infected with 2 groups, 2 snail species B. fuchsiana and T. granifera were infected with one group of cercaria. The results of ITS2 sequences analyses of the cercarial groups identified the larvae of 9 trematode species, namely Echinostoma revolutum, Echinochasmus japonicus, Notocotylus intestinalis, Philophthalmus gralli, Haplorchis pumilio, Procerovum cheni, Fasciola gigantica, Australapatemon burti and Cyathocotyle prussica. Among them the last three species, P. cheni, A. burti and C. prussica, were found for the first time in Vietnam. In addition, the ITS-2 sequence of Gymnocephalous cercariae which was previously identified as Sphaeridiotrema monorchis, from P. striatulus snail was 97% similar to that of Sphaeridiotrema pseudoglobulus. Likewise, ITS-2 sequence of Echinostome cercaria from B. fuchsiana snail was 93% similar to that of E. japonicus and that of Xiphidiocercaria cercaria from M. tuberculata snail was 93% similar to Lecithodendrium spathulatum.

Trematode larval infections in snails collected from aquaculture ponds in Ha Noi and Yen Bai, Vietnam

“…Up to present, the gymnocephalous cercariae of three species i.e. Fasciola gigantica, Sphaeridiotrema monorchis and S. pseudoglobulus have been identified by the experimental infection or molecular analyses (Besprozvannykh et al, 2013;Doanh et al 2019). Regarding the snail host, Radix auriculata is of great parasitological importance as it includes several intermediate hosts of trematodes which infect man, mammals, e.g.…”

Digenean infections in snails (cercariae stage) in Can Gio Mangrove Biosphere Reserve, Ho Chi Minh City, Vietnam

“…In 2015, three cercariae groups are recovered from 7 snail species, in which Melanoides tuberculata has the highest prevalence [ 34 ]. Nine snail species infected with cercariae in Ninh Binh province and Ha Noi city [ 35 ].…”

Pesticidal Activities of Callicarpa and Premna Essential Oils From Vietnam

“…Gyraulus convexiusculus (Hutton) is an intermediate host for several trematode parasites 13,14 including Echinostoma revolutum , Australapatemon burti , 15 Artyfechinostomum malayanum (intestinal flukes), 16 Sanguinicola armata (blood fluke), 17 and Cercaria sp. 18 In addition, G convexiusculus is an intermediate host of Olveria indica , an amphistome parasite.…”

“…Moreover, P. canaliculata is an intermediate host for human parasites such as Angiostrongylus cantonensis, which leads to eosinophilic meningitis, 9 Gnathostoma spinigerum, which is the cause of gnathostomiasis, 10 and Angiostrongylus vasorum which leads to eosinophilic encephalitis, 11 and the intestinal fluke Echinostoma ilocanum. 12 Gyraulus convexiusculus (Hutton) is an intermediate host for several trematode parasites 13,14 including Echinostoma revolutum, Australapatemon burti, 15 Artyfechinostomum malayanum (intestinal flukes), 16 Sanguinicola armata (blood fluke), 17 and Cercaria sp. 18 In addition, G convexiusculus is an intermediate host of Olveria indica, an amphistome parasite.…”