Infection of the One-Day Old Fertile Hen's Egg With Rabies Virus

Yoshino, Kamesaburo; Kuma, Nobuaki; Kondo, Akira; Kitaoka, Masami

doi:10.7883/yoken1952.9.259

Cited by 18 publications

(11 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another CE passage line of this strain had been supplied by Dr. A. Kondo, National Institute of Health, Tokyo, which represented the 20th CE passage when received and was further passaged in CE cells using neutral maintenance medium in this laboratory up to the 150th passage level. Three other strains of rabies virus [46] had the following passage history before adaptation to CE cells : Nishigahara, 1848 rabbit brain, 4 adult mouse brain, 30 one-day egg and then 17 adult mouse brain passages; CVS, 64 adult mouse brain and 42 one-day egg passages; and Takamen, 12 adult mouse brain and 12 suckling mouse brain passages. The three strains were further passaged in CE cells using neutral maintenance medium as indicated in Results.…”

Section: And Methodsmentioning

confidence: 99%

Alteration of the In Vitro Host Range of Rabies Virus after Serial Chick Embryo Cell Passage Using Alkaline Maintenance Medium

Hashimoto

Yoshino

1976

Japanese Journal of Microbiology

Self Cite

View full text Add to dashboard Cite

HEP Flury strain of rabies virus maintained by 7-day chicken egg passage (parent line) and the same strain serially passaged in primary chick embryo (CE) cells using alkaline maintenance medium (AM line) were inoculated to cells of various species. Growth was negative in primary mouse embryo, L and HeLa cells, and positive in primary hamster kidney and BHK21 cells with both lines. An allor-none difference between the two lines was observed in primary monkey kidney and Vero cells. The parent line did not multiply in these monkey cells, whereas the AM line grew to high titers. In the case of Vero cells a unique cytopathic effect (CPE) was induced by the AM line. After five consecutive passages in Vero cells, the CPE-inducing agent was identified as rabies virus by a neutralization test. It was infective to intracerebrally inoculated suckling mice but not to adult mice, and its Vero cell-infective titer determined by CPE induction was about 1 log lower than the baby mouse-infective and CE plaque-forming titers. In contrast to the AM line, HEP Flury strain receiving 150 CE cell passages under neutral maintenance medium and three other strains receiving similar CE cell passages all failed to grow in Vero cells.

show abstract

Section: And Methodsmentioning

confidence: 99%

Alteration of the In Vitro Host Range of Rabies Virus after Serial Chick Embryo Cell Passage Using Alkaline Maintenance Medium

Hashimoto

Yoshino

1976

Japanese Journal of Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Materials and methods were essentially the same as detailed in the foregoing paper (Yoshino et al, 1956), except for the following points. FOP and COP viruses: Both Flury and CVS strains were serially passed through 1-day eggs as described previously (Yoshino et al, 1956…”

Section: Methodsmentioning

confidence: 99%

“…The A seed of COP virus was serially diluted and injected into 25 mice and/or here that these 1-day egg-adapted viruses showed about one log higher 1-day egg ID50 units than m.i.u. (Yoshino et al, 1956). Therefore, it was thought to be a reasonable estimation that the 1-day egg LD50 faithfully represented the 1-day egg ID50.…”

Section: Inoculation At Different Sites With 1-day Egg-adapted and Nomentioning

confidence: 98%

“…The ID50 determination was done in a manner identical with that previously performed (Yoshino et al, 1956). This time, however, a special consideration was taken on the possibility that a slight shift in the inoculation site might change the ID50 titer.…”

Section: Inoculation At Different Sites With 1-day Egg-adapted and Nomentioning

confidence: 99%

“…Since it was demonstrated that the embryos of 1-day eggs infected with rabies virus were apt to die within one week (Yoshino et al, 1956), attempts have been made to develop a method for viral titration by inoculation of 1-day eggs. However, two difficulties were encountered: firstly, the eggs inoculated with a considerable amount of virus did not necessarily die within a short period of time, and secondly control eggs receiving diluent only also showed a high percentage of non-specific deaths.…”

mentioning

confidence: 99%

See 2 more Smart Citations