Infection of the one-day old fertile hen's egg with rabies virus

Yoshino, Kamesaburo; Kondo, Akira; Kuma, Nobuaki; Taniguchi, Hirokazu

doi:10.1007/bf01241645

Cited by 10 publications

(1 citation statement)

References 10 publications

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“…It is worth noting that the techniques used by Yoshino and his co-workers for these studies have many advantages in economy and speed of handling. The use of the 1-day-old egg enables the presence or absence of neutralizing antibodies to be detected in 5 days, because in the presence of infectious virus the blastoderm ceases to grow (87). The titration of the neutralizing activity of each serum can be performed on a chick embryo monolayer growing in a single petri dish (77).…”

Section: Serological Epidemiologymentioning

confidence: 99%

Herpesvirus Hominis (Virus of Herpes Simplex)1

Scott¹,

Tokumaru²

1964

Bacteriological Reviews

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Section: Serological Epidemiologymentioning

confidence: 99%

Herpesvirus Hominis (Virus of Herpes Simplex)1

Scott¹,

Tokumaru²

1964

Bacteriological Reviews

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Infection of the one-day old fertile hen's egg with rabies virus

Yoshino

Suzuki

Kondo

1961

Archiv f Virusforschung

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Plaque assay of rabies virus in chick embryo cells

Yoshino

Taniguchi

Arai

1966

Archiv f Virusforschung

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It was attempted to establish a plaque assay method for rabies virus using primary chick embryo (CE) cell monolayers. Independently of our investigation, Kondo (1) found plaque formation in CE cells of a CE fibroblast-adapted line of the HEP Flury strain (2). However, according to his result, the incubation period required for the development of plaques was not constant, varying from l0 to 20 days depending upon each test. In contrast, our present results show that plaques become visible in a constant period of 6 days, and that the HEP Flury strain need not be further adapted to cultured CE fibroblasts in order to be able to form plaques in CE cells.Nine-day chick embryos were trypsinized by the method described elsewhere (3), suspended in a growth mediu~t (Earle~s balanced salt solution supplemented with 0.5% lactalbumin hydrolysate, 0.1~o yeast extract, 10% calf serum, 0.01 M Tris buffer of pH 7.2, and antibiotics) at a concentration of 2,000,000 cells per ml. and sown in 5 ml. amounts into 60 mm. petri dishes. In this procedure, it was essential to minimize the time lapse during which the cells were placed in the cold. After 24 hours' incubation at 37 ~ C, the dishes were washed once with PBS (4) and received 0.05 ml. per dish of virus inocula, which had been made by diluting a seed virus (a 20% emulsion of brains of chick embryos which had been infected at the age of 7 days with the HEP Flury strain) with 0.1 ~ yolk-saline (3). The dishes were incubated at 37 ~ C for one hour for * This study was supported in part by UoS. PHS grant AI-04248 and a grant from the World Health Organization. F i g . 1. P l a q u e s f o r m e d i n C E cells b y I~E P F l u r y s t r a i n of r a b i e s v i r u s . T r i s b u f f e r s of p i t 7.2, 7.8 a n d 8.6 w e r e i n c o r p o r a t e d i n t h e o v e r l a y m e d i a , as i n d i c a t e d . ~ C~ m" 9 6. Yoshino, K., A. Kondo, iV. Kuma, and H. Taniguchi: Infection of the oneday old fertile hen's egg with rabies virus VI. Strain differences in the egg adaptability, Arch. ges. Virusforsch. 10~ 684--697 (1960).

show abstract

Infection of the one-day old fertile hen's egg with rabies virus

Cited by 10 publications

References 10 publications

Herpesvirus Hominis (Virus of Herpes Simplex)1

Herpesvirus Hominis (Virus of Herpes Simplex)1

Infection of the one-day old fertile hen's egg with rabies virus

Plaque assay of rabies virus in chick embryo cells

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