Infection of rat cells by avian sarcoma virus: Factors affecting transformation and subsequent reversion

Wyke, John A.; Quade, Kristina

doi:10.1016/0042-6822(80)90246-9

Cited by 29 publications

(31 citation statements)

References 36 publications

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“…ASV transformants A typical experiment like that illustrated in Figure 1 using All VIT, a Rat-I cell transformed by ASV is illustrated in Figure 4. After digestion with EcoRI, three bands are detected by the ASV probe, the top one being a doublet (Wyke and Quade, 1980). The bands obtained with the DNA purified from cages that retained only 4% of the total are at least 3 x more intense than the control bands (Figure 4, compare channels 3 and 4 with 1, 2, and 9-11).…”

Section: Integrated Polyoma Sequencesmentioning

confidence: 99%

“…The derivation of the polyoma and ASV transformants has been described (Lania et al, , 1980Wyke and Quade, 1980;Varmus et al, 1981;Chiswell et al, 1982).…”

Section: Cellsmentioning

confidence: 99%

“…taining viral sequences integrated at one different cellular site, were obtained by transformation of Rat-I cells (Mishra and Ryan, 1973) with polyoma (Lania et al, , 1980 or ASV (Wyke and Quade, 1980;Chiswell et al, 1982;Varmus et al, 1981). One mouse line transformed by polyoma (i.e., Tsa 3T3) was also included (Lania et al, , 1980.…”

Section: Introductionmentioning

confidence: 99%

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Active viral genes in transformed cells lie close to the nuclear cage.

Cook¹,

Lang²,

Hayday³

et al. 1982

The EMBO Journal

108

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Nuclear DNA is looped by attachment to a matrix or cage. Using nine different lines transformed by polyoma or avian sarcoma virus, we have mapped viral sequences integrated within these loops. In all lines that contain high concentrations of viral transcripts and express the transformed phenotype, the integrated viral genes lie close to the points of attachment to the cage. Integration of polyoma DNA induces outlying cellular sequences to become closely associated with the cage. The strength of this correlation between gene activity and proximity to the cage was examined using sub‐clones of one avian sarcoma virus transformant. Proviral sequences are closely associated with the cage in this transformant, much less so in two untransformed ‘flat revertants’ which contain no detectable viral transcripts but regain their close association with the cage in two retransformed derivatives.

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Section: Integrated Polyoma Sequencesmentioning

confidence: 99%

“…The derivation of the polyoma and ASV transformants has been described (Lania et al, , 1980Wyke and Quade, 1980;Varmus et al, 1981;Chiswell et al, 1982).…”

Section: Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Active viral genes in transformed cells lie close to the nuclear cage.

Cook¹,

Lang²,

Hayday³

et al. 1982

The EMBO Journal

108

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“…Chicken embryo fibroblasts (CEF) were derived from chicken factor-negative eggs (Lohmann, Cuxhaven, F.R.G.). CEF, normal rat kidney (NRK) and rat-1 cells were cultured as described previously (Wyke & Kurth, 1978;Wyke & Quade, 1980;Ellwart-Tschiirtz & Kurth, 1981). Temperature-sensitive (ts) transformation-defective (td) RSV mutants were used to infect CEF maintained at permissive (37 °C) or restrictive (41 °C) temperature.…”

Section: --/Omentioning

confidence: 99%

“…RSV of an appropriate envelope subgroup efficiently penetrates mammalian cells, but the level of expression of structural and transforming viral genes in mammalian cells is generally lower and the ratios of viral RNAs are different from those observed in permissive avian cells. Mammalian cells are usually non-permissive for virus replication and, despite DNA provirus integration (Boettiger et at., 1975;Varmus et at., t975), malignant cell transformation appears to be an infrequent and reversible event (Wyke & Quade, 1980). Although the majority of RSV-infected mammalian cells remain normal, transformed descendents can be isolated after prolonged cultures.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Monoclonal Antibodies Specific for Rous Sarcoma Virus Structural, Polymerase and Transforming Proteins and Their Use for the Study of Mutant Virus-infected Cells

Kurth

Tanaka

Moelling

1985

Journal of General Virology

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SUMMARYMonoclonal antibodies were developed that are specific for Rous sarcoma virus structural, polymerase (reverse transcriptase) and transforming proteins. The monoclonal antibodies were shown to bind to purified virus proteins in an indirect ~ :5I-labelled Protein A binding assay suitable for screening even very large numbers of hybridomas. Additional tests for specificity included radioimmunoprecipitation of purified virus structural proteins P 12 and P27, of reverse transcriptase subunits ~ and/~, and of the transforming protein pp60 ..... . Pilot immunofluorescence and protein kinase assays of the expression of virus proteins in avian and mammalian cells infected by wild-type virus as well as by temperature-sensitive, transformation-defective virus mutants revealed that synthesis of virus structural and transforming proteins is hardly affected by changes in temperature, whereas the pp60"-~c-associated kinase activity is temperature-sensitive in cells infected by most, but not all the virus mutants.

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