Infection of novel reassortant H1N2 and H3N2 swine influenza A viruses in the guinea pig model

Tapia, Rodrigo; García, Victòria Reyes; Mena, Juan; Bucarey, Sergio A.; Medina, Rafael; Neira, Víctor

doi:10.1186/s13567-018-0572-4

Cited by 16 publications

(22 citation statements)

References 23 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In cats, at the last sampling, serum samples were also collected. Nasal samples were collected using flocked swabs; fresh feces were stored in ziplock bags [16][17][18] and deposited in viral transport media.…”

Section: Case Description and Specimen Collectionmentioning

confidence: 99%

A household case evidences shorter shedding of SARS-CoV-2 in naturally infected cats compared to their human owners

Neira

Brito

Agüero

et al. 2021

Emerging Microbes & Infections

Self Cite

View full text Add to dashboard Cite

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been detected in domestic and wild cats. However, little is known about natural viral infections of domestic cats, although their importance for modelling disease spread, informing strategies for managing positive human-animal relationships and disease prevention. Here, we describe the SARS-CoV-2 infection in a household of two human adults and sibling cats (one male and two females) using real-time RT–PCR, an ELISA test, viral sequencing, and virus isolation. On May 5th, 2020, the cat-owners tested positive for SARS-CoV-2. Two days later, the male cat showed mild respiratory symptoms and tested positive. Four days after the male cat, the two female cats became positive, asymptomatically. Also, one human and one cat showed antibodies against SARS-CoV-2. All cats excreted detectable SARS-CoV-2 RNA for a shorter duration than humans and viral sequences analysis confirmed human-to-cat transmission. We could not determine if cat-to-cat transmission also occurred.

show abstract

Section: Case Description and Specimen Collectionmentioning

confidence: 99%

A household case evidences shorter shedding of SARS-CoV-2 in naturally infected cats compared to their human owners

Neira

Brito

Agüero

et al. 2021

Emerging Microbes & Infections

Self Cite

View full text Add to dashboard Cite

show abstract

“…The HA sequences from clusters ChH1A and ChH1B were previously classified within the human seasonal lineage Other-Human-1B.2 26 , according to the global swine H1 clade classification 13 . However, although both were of the Other-Human-1B.2 global clade, ChH1A and ChH1B were vastly distinct genetically.…”

Section: Discussionmentioning

confidence: 99%

“…The HA segments of these Chilean IAVs were likely introduced into swine from humans in the late 1980s and early 1990s 24 . Hence, these viruses evolved in the swine population for over two decades, and now they are genetically distant from other human IAVs described worldwide [24][25][26] . Moreover, these Chilean strains have unique glycosylation site patterns, which were not detected in human IAV strains 20 .…”

Section: Discussionmentioning

confidence: 99%

“…Cells were then rinsed with 1x phosphate-buffered saline (PBS) to remove unbound virus, and IAV growth medium was added. Cells were incubated at 37 °C and observed for cytopathic effect (CPE) daily for 5 days 26 . CPE positive samples were tested by real time RT-PCR to confirm the presence of IAV.…”

mentioning

confidence: 99%

“…Four-week-old female guinea pigs, Pirbright strain, free of IAV and IAV antibodies, were obtained from the Instituto de Salud Pública de Chile. They were acclimated for 7 days on a 12 h light/dark cycle and were allowed access to food and water ad libitum 26 . Animals were then randomly separated in experimental groups of 2 animals, which were immunized subcutaneously with 0.2 mL of each inactivated IAV vaccine.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Antigenic characterization of novel H1 influenza A viruses in swine

Tapia

Torremorell

Culhane

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Novel H1N2 influenza A viruses (IAVs) in swine have been identified in Chile co-circulating with pandemic H1N1 2009-like (A(H1N1)pdm09-like) viruses. The objective of this study was to characterize antigenically the swine H1 IAVs circulating in Chile. Genetic analysis based on the HA1 domain and antigenic analysis by hemagglutination inhibition assay were carried out. Three antigenic clusters were identified, named Chilean H1 A (ChH1A), Chilean H1 B (ChH1B), and A(H1N1)pdm09-like. The antigenic sites of ChH1A and ChH1B strains were 10-60% distant from those of commercial vaccine strains at the amino acid sequence level. Antigenic variants were identified within the clusters ChH1A and A(H1N1)pdm09-like. Substitutions in the main antigenic sites (E153G in Sa, Q193H in Sb, D168N in Ca1, P137S in Ca2, and F71L in Cb) were detected in variants from the ChH1A cluster, whereas only a single substitution in antigenic site Sa (G155E) was detected in variants from A(H1N1)pdm09-like cluster, which confirms the importance to carrying out antigenic analyses in addition to genetic analyses to evaluate control measures such as vaccination. These results highlight the need to update vaccines for swine in Chile and the importance of continued surveillance to determine the onward transmission of antigenic variants in Chilean pig populations. Influenza A virus (IAV) is a member of the family Orthomyxoviridae possessing 8 negative sense single-stranded RNA segments 1 and classified in subtypes based on the antigenicity of their surface glycoproteins: 18 subtypes for hemagglutinin (HA) and 11 subtypes for neuraminidase (NA) 2. IAV can infect birds and several mammalian species, including human and swine. Pigs have an important role in the ecology of IAV, since they can become infected with both human and avian strains 3,4. Co-infection with IAVs from different lineages can generate reassortant strains with potential epidemic and zoonotic risks 5-7. IAVs are ubiquitous in swine worldwide, generating significant economic losses and representing a public health concern 8,9. The main control measure in many swine farms is the use of vaccines, but the commercial vaccines currently available are based on North American or European IAV strains 10,11. H1N1, H1N2 and H3N2 are the main subtypes circulating in swine globally; however, IAVs in swine are genetically and antigenically diverse even within each subtype, and several lineages have been reported 12,13. This IAV diversity is the result of genetic evolution and antigenic changes that occur primarily through 2 mechanisms: 'antigenic shift' , by reassortment of gene segments encoding surface glycoproteins, HA and NA; and 'antigenic drift' , by non-synonymous substitutions in these glycoproteins, mainly in the antigenic sites of the HA, against which neutralizing antibodies against IAV are predominantly generated 14,15. The HA is organized as a non-covalent homo-trimer on the viral surface where each monomer consists of two polypeptides, HA1 and HA2 16. HA1 is the major immunogenic polypept...

show abstract

Syntheses and Applications of Nanomaterials-Based Photocatalysts for Air Purification

Haliq

Tarigan

Aprilio

et al. 2022

Photocatalytic Activities for Environmental Remediation and Energy Conversion

View full text Add to dashboard Cite

Infection of novel reassortant H1N2 and H3N2 swine influenza A viruses in the guinea pig model

Cited by 16 publications

References 23 publications

A household case evidences shorter shedding of SARS-CoV-2 in naturally infected cats compared to their human owners

A household case evidences shorter shedding of SARS-CoV-2 in naturally infected cats compared to their human owners

Antigenic characterization of novel H1 influenza A viruses in swine

Syntheses and Applications of Nanomaterials-Based Photocatalysts for Air Purification

Contact Info

Product

Resources

About