Infection detection in mice using 99mTc-labeled HYNIC and N2S2 chelate conjugated to the antimicrobial peptide UBI 29-41

Welling, Mick M.; Visentin, R.; Feitsma, H. I. J.; Lupetti, Antonella; Pauwels, E. K. J.; Nibbering, Peter H.

doi:10.1016/j.nucmedbio.2003.11.009

Cited by 36 publications

(28 citation statements)

References 8 publications

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“…3). 43 Besides the above mentioned labeling methods, 99m Tc-labeling is also possible using the reducing conditions under which the þ7 oxidation state in pertechnetate is reduced to the þ5 oxidation state. This is problematic for peptides containing intramolecular disulfide bonds which are usually reduced resulting in altered chemical structure and altered biodistribution and biological activity.…”

Section: Labeling Of Peptides With Technetium-99mmentioning

confidence: 99%

The Pharmacology of Radiolabeled Cationic Antimicrobial Peptides

Brouwer

Wulferink

Welling

2008

Journal of Pharmaceutical Sciences

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Section: Labeling Of Peptides With Technetium-99mmentioning

confidence: 99%

The Pharmacology of Radiolabeled Cationic Antimicrobial Peptides

Brouwer

Wulferink

Welling

2008

Journal of Pharmaceutical Sciences

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“…8,9 In view of the incomplete knowledge we considered a labelling method via the well-accepted chelator HYNIC attached to the amine terminal of UBI 29-41 during synthesis while other amine residues were protected from multiple conjugations. This approach provides a well-understood labelling mechanism that lends itself for easy kit preparation.…”

Section: Accumulation In Bacterial Infected Thigh Musclesmentioning

confidence: 99%

“…Samples of 10-50 ml of the labelled samples were analysed on a reverse-phase HPLC column (Dionex 4.6 Â 250 mm C 18 column, Vydac, The Separations Group, Hesperia, CA) using a linear gradient as described 8 at a flow rate of 1 ml/min.…”

Section: Labellingmentioning

confidence: 99%

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Kit with technetium‐99m labelled antimicrobial peptide UBI 29‐41 for specific infection detection

Welling

Korsak²,

Górska³

et al. 2005

Labelled Comp Radiopharmac

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SummaryThe purpose of this study was to investigate radiochemical and biological characteristics of an instant kit for the preparation of TcO 4 À to the kit ITLC and HPLC reversephase analysis was performed. Stability of the labelled complex was challenged and the binding to bacterial pellets was assessed. Finally, the biodistribution and accumulation in MRSA-infected tissues were studied using scintigraphy and ex vivo countings. Data were compared to a non-kit control method.Radiochemical analysis indicated >96% labelling, stability for 24 h and the preparation was used without purification. In vitro studies showed 41% of radioactivity was bound to bacteria. After injection into mice with a bacterial infection the site of infection was visualized within 30 min. Kit prepared

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“…In this respect, a number of radiolabeled peptides have been assessed for the scintigraphic recognition of infections [8,9]. The most promising ones among them are 99m Tc-labeled cationic antimicrobial peptides originating from ubiquicidin (UBI), which remarkably bind to microorganisms [10,11].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic value of 99mTc-ubiquicidin scintigraphy for osteomyelitis and comparisons with 99mTc-methylene diphosphonate scintigraphy and magnetic resonance imaging

Assadi

Vahdat²,

Nabipour³

et al. 2011

Nuclear Medicine Communications

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Objective The discrimination of bacterial infections from sterile inflammatory processes is of great importance in the management of inflammation. Currently available techniques cannot decisively address this issue. In this respect, antimicrobial peptide 99m Tc-ubiquicidin (UBI) 29-41 scans have been showing interesting results. The aim of this study was to determine the accuracy of 99m Tc-UBI scan in the detection of osteomyelitis and to compare it with 99m Tc-methylene diphosphonate scan and magnetic resonance imaging (MRI).Methods Twenty patients (mean age = 48.90 years) with suspected osteomyelitis were included in this study. After evaluation of each patient through history taking, physical examination, appropriate laboratory tests, and other processes including bone probing, wound culture, and plain film radiography, MRIs, 99m Tc-UBI scans, and 99m Tc-methylene diphosphonate scans were performed. For quantitative analysis, the mean count of abnormal-tonormal (A/N) region was calculated for images acquired at 15, 30, 45, 60, 120, and 240 min to obtain the most favorable time for imaging. Results In total, osteomyelitis was detected in the 99mTc-UBI scans of 17 patients, indicating 100% accuracy, compared with an accuracy of 90% for osteomyelitis detected in three-phase bone scans. The maximum mean A/N was observed at 15 min after intravenous injection (median: 1.91; interquartile range: 1.54-2.94). MRI was performed in 12 cases only with 75% accuracy. In addition, the A/N ratios for the 99m Tc-UBI scans were not significantly different between patients with or without Staphylococcus aureus growth on wound cultures.Conclusion For fast imaging with high accuracy, 99m Tc-UBI 29-41 is a suitable choice for the detection of osteomyelitis.

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Infection detection in mice using 99mTc-labeled HYNIC and N2S2 chelate conjugated to the antimicrobial peptide UBI 29-41

Cited by 36 publications

References 8 publications

The Pharmacology of Radiolabeled Cationic Antimicrobial Peptides

The Pharmacology of Radiolabeled Cationic Antimicrobial Peptides

Kit with technetium‐99m labelled antimicrobial peptide UBI 29‐41 for specific infection detection

Diagnostic value of 99mTc-ubiquicidin scintigraphy for osteomyelitis and comparisons with 99mTc-methylene diphosphonate scintigraphy and magnetic resonance imaging

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