Industrial soil is susceptible to acid or alkali pollution, but studies focused on the remediation of such soil are still limited. This manuscript investigated the neutralization effect of five agents (hydrochloric acid, citric acid, ferrous sulfate, calcium superphosphate and raw gypsum) to alkali polluted soil. The results showed that regarding the initial pH after the neutralizing agent addition, it was better to set it lower than the target, as the pH would rebound. None of the five agents caused an obvious increase in the heavy metal contents of the leachates, but they all caused an increase in electrical conductivity, which indicated an increase in soil salinity. The leachates showed a luminous gain to Vibrio fischeri. However, remediation with hydrochloric acid would cause significant inhibition of germination and root elongation of pakchoi. In addition, the addition of neutralizing agents reshaped the soil microbial community structure in different patterns. Soils treated with hydrochloric acid and ferrous sulfate seemed to improve the microbial richness. The neutralization might be favorable for the biodegradation of polycyclic aromatic hydrocarbons (PAHs), which usually coexist in industrial contaminated soil. In general, the neutralization of alkaline industrial soils using ferrous sulfate, superphosphate and gypsum brought minimal environmental risk, among which ferrous sulfate was the first recommendation in industrial soil after a comprehensive comparison.