Industrial Genotoxicology Group collaborative trial to investigate cell cycle parameters in human lymphocyte cytogenetics studies

Henderson, L.M.; Jones, E.; Brooks, T.M.; Chételat, Andreé-Anne; Chiliutti, P.; Freemantle, M.; Howard, C. Anthony; Mackay, James; Phillips, Barry; Riley, Sue; Roberts, Charles; Wotton, A.K.; Waart, E.J. van de

doi:10.1093/mutage/12.3.163

Cited by 22 publications

(11 citation statements)

References 15 publications

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“…For many years, human lymphocyte cultures have been used to assess the clastogenic potential of test substances in vitro (Henderson et al, 1997). However, since many drugs are (in)activated after their metabolization, tests in vivo of mutagenicity are recommended for assessing the clastogenic activity of certain compounds whose activity requires metabolic activation; such an approach also provides conditions closer to those found in humans (Legator and Ward Jr., 1991).…”

Section: Discussionmentioning

confidence: 99%

Genotoxic action of the sesquiterpene lactone glaucolide B on mammalian cells in vitro and in vivo

Burim¹,

Canalle²,

Lopes³

et al. 1999

Genet. Mol. Biol.

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Glaucolide B is a sesquiterpene lactone isolated from Vernonia eremophila Mart. (Vernonieae, Asteraceae) and has schistosomicidal, antimicrobial and analgesic activities. This study examined the cytotoxic and clastogenic activities of glaucolide B in human cultured lymphocytes and in bone marrow cells from BALB/c mice. The mitotic index (MI) and chromosomal aberrations were analyzed in both of the above systems, whereas sister chromatid exchanges (SCE) and the proliferation index (PI) were determined only in vitro. In human cultured lymphocytes, glaucolide B concentrations greater than 15 µg/ml of culture medium completely inhibited cell growth. At 4 µg/ml and 8 µg/ml of culture medium, glaucolide B significantly increased the frequency of chromosomal aberrations in lymphocytes and was also cytotoxic at concentrations ≥8 µg/ml; there was no increase in the frequency of SCE. Glaucolide B (160-640 mg/kg) did not significantly increase the frequency of chromosomal aberrations in mouse bone marrow cells nor did it affect cell division. Since glaucolide B showed no clastogenic action on mammalian cells in vivo but was cytotoxic and clastogenic in vitro, caution is needed in its medicinal use.

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Section: Discussionmentioning

confidence: 99%

Genotoxic action of the sesquiterpene lactone glaucolide B on mammalian cells in vitro and in vivo

Burim¹,

Canalle²,

Lopes³

et al. 1999

Genet. Mol. Biol.

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“…All viabilities were measured by trypan blue exclusion to avoid artefacts due to toxicity (Henderson et al 1997). The cut-off point suggested by Henderson et al (1997) was 70%.…”

Section: Viabilitiesmentioning

confidence: 99%

The effect of zinc oxide and titanium dioxide nanoparticles in the Comet assay with UVA photoactivation of human sperm and lymphocytes

et al. 2009

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The present study has examined the effects of ZnO and TiO 2 nanoparticles (40Á70 nm range) in the presence and absence of UVA light in human sperm and human lymphocytes in the dark (D), after pre-irradiation with UV (PI) and simultaneous irradiation with UV (SI). For both ZnO and TiO 2 nanoparticles, the percentage reduction in head DNA was greater for PI and SI samples compared with samples treated in the dark. However with regard to photogenotoxicity, sperm exhibited no significant differences when the results for PI and SI and the dark were compared, except at the lowest concentration for SI samples in the case of ZnO and the lowest concentration for PI in the case of TiO 2 . However for lymphocytes, responses were statistically significant at the highest concentration for both PI and SI samples. Thus, these studies suggest that there are photogenotoxic events in these cells in the absence of overt toxicity.

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“…Donors were not taking any form of medication. The measured cell cycle time of the donors estimated in our laboratory falls within the range 13± 2 h [38]. For the main experiment, aliquots of whole blood (0.5 ml) from each donor were added, separately, to sterile disposable centrifuge tubes containing 4.5 ml RPMI-1640 culture medium supplemented with 20% heatinactivated fetal calf serum (Gibco BRL), 100 U/ml Hepes buffer, 2 mM l-glutamine and antibiotics (1% w/v penicillin and 100 M streptomycin).…”

Section: Cells and Culture Conditionsmentioning

confidence: 62%

Cytogenetic evidence that DNA topoisomerase II is not involved in radiation induced chromsome-type aberrations

Mosesso

Pepe

Ottavianelli

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Industrial Genotoxicology Group collaborative trial to investigate cell cycle parameters in human lymphocyte cytogenetics studies

Cited by 22 publications

References 15 publications

Genotoxic action of the sesquiterpene lactone glaucolide B on mammalian cells in vitro and in vivo

Genotoxic action of the sesquiterpene lactone glaucolide B on mammalian cells in vitro and in vivo

The effect of zinc oxide and titanium dioxide nanoparticles in the Comet assay with UVA photoactivation of human sperm and lymphocytes

Cytogenetic evidence that DNA topoisomerase II is not involved in radiation induced chromsome-type aberrations

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