Inductive immune responses in the Japanese pufferfish (Takifugu rubripes) treated with recombinant IFN-γ, IFN-γrel, IL-4/13A and IL-4/13B

Biswas, Gouranga; Nagamine, R.; Hikima, Jun-ichi; Sakai, Masahiro; Kono, Tomoya

doi:10.1016/j.intimp.2015.12.005

Cited by 20 publications

(3 citation statements)

References 32 publications

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“…In zebrafish embryos, knockdown of the IFN-γrel1 (IFN-γ1) and its cognate receptor CRFB13 by morpholinos results in a significant reduction of embryo survival after infection with the bacterial pathogen Yersinia ruckeri , suggesting it plays an important role in innate defence against bacterial infection [ 263 ]. When injected intramuscularly into Fugu, IFN-γrel increases expression of IL-1β, IL-6, p35, p40, and IFN-γ, which are important mediators of immune responses against bacterial infection [ 264 ]. In Fugu, IFN-γrel and IFN-γ appear to trigger similar responses, including phagocytic activity and lysozyme activity.…”

Section: Type II α Helical Cytokinesmentioning

confidence: 99%

The Function of Fish Cytokines

Zou

Secombes

2016

Biology

428

269

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What is known about the biological activity of fish cytokines is reviewed. Most of the functional studies performed to date have been in teleost fish, and have focused on the induced effects of cytokine recombinant proteins, or have used loss- and gain-of-function experiments in zebrafish. Such studies begin to tell us about the role of these molecules in the regulation of fish immune responses and whether they are similar or divergent to the well-characterised functions of mammalian cytokines. This knowledge will aid our ability to determine and modulate the pathways leading to protective immunity, to improve fish health in aquaculture.

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Section: Type II α Helical Cytokinesmentioning

confidence: 99%

The Function of Fish Cytokines

Zou

Secombes

2016

Biology

428

269

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“…Stimulation of tetraodon spleen and head kidney cells in vitro with IFN-γrel2 (termed IFN-γ1 in this paper) enhanced their nitric oxide responses and expression of ISG15 ( 58 ). Injection of IFN-γrel2 into Japanese pufferfish has been shown to increase phagocyte function in terms of phagocytosis and ROS production, and IFN-γ expression in head kidney cells 1 day post-injection, with a longer term effect seen on IL-6 and IL-12p35/IL-12p40 gene expression ( 59 ). It is clear that IFN-γrel is an important immune molecule within the immune system of teleost fish.…”

Section: Ifn-γ Relatedmentioning

confidence: 99%

Evolution of Interferons and Interferon Receptors

2017

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The earliest jawed vertebrates (Gnathostomes) would likely have had interferon (IFN) genes, since they are present in extant cartilaginous fish (sharks and rays) and bony fish (lobe-finned and ray-finned fish, the latter consisting of the chondrostei, holostei, and teleostei), as well as in tetrapods. They are thought to have evolved from a class II helical cytokine ancestor, along with the interleukin (IL)-10 cytokine family. The two rounds of whole genome duplication (WGD) that occurred between invertebrates and vertebrates (1) may have given rise to additional loci, initially containing an IL-10 ancestor and IFN ancestor, which have duplicated further to give rise to the two loci containing the IL-10 family genes, and potentially the IFN type I and IFN type III loci (2). The timing of the divergence of the IFN type II gene from the IL-10 family genes is not clear but was also an early event in vertebrate evolution. Further WGD events at the base of the teleost fish, and in particular teleost lineages (cyprinids, salmonids), have duplicated the loci further, giving rise to additional IFN genes, with tandem gene duplication within a locus a common occurrence. Finally, retrotransposition events have occurred in different vertebrate lineages giving rise to further IFN loci, with large expansions of genes at these loci in some cases. This review will initially explore the likely IFN system present in the earliest Gnathostomes by comparison of the known cartilaginous fish genes with those present in mammals and will then explore the changes that have occurred in gene number/diversification, gene organization, and the encoded proteins during vertebrate evolution.

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“…A cell line expressing IL-4/13B has been obtained from carp ( Cyprinus carpio ) to investigate in vitro Th2 fish immune responses 25 . Finally, a possible Th2 inflammatory process has been discovered in coho salmon ( Oncorhynchus kisutch ) after a disease outbreak due to the salmon louse ( Lepeophtheirus salmonis ) 26 and recombinant IL-4/13 molecules have been produced in zebrafish 27 , carp 28 , rainbow trout 24 , Japanese pufferfish 29 and goldfish 30 to investigate the fish Th2-like regulatory mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity

Stocchi

Wang

Randelli

et al. 2017

Sci Rep

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Th2 immunity is a primary host defence against metazoan pathogens and two of the important cytokines involved in this immune response in mammals are IL-4 and IL-13. Recently the origin and evolution of Th2 immune responses have been investigated in fish where a molecule with relatedness to both IL-4 and IL-13 is present, termed IL-4/13. Different IL-4/13 paralogues (IL-4/13 A and IL-4/13B) exist in teleost fish. In this paper, we have focused on the IL-4/13 isoforms found in the European sea bass (Dicentrarchus labrax L.). Two tandem duplicated but divergent IL-4/13 A isoforms and one IL-4/13B are present, a unique situation compared to other teleosts. These genes were studied in terms of their in vitro and in vivo transcript levels after different treatments and their biological activities after production of the recombinant isoforms. The results show that the presence of these three paralogues is associated with different activities, both in terms of their expression profiles and the ability of the proteins to modulate the expression of immune genes in head kidney leukocytes. It is clear that the initiation and control of type-2 responses in seabass is complex due to the presence of multiple IL-4/13 isoforms with overlapping but distinct activities.

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Inductive immune responses in the Japanese pufferfish (Takifugu rubripes) treated with recombinant IFN-γ, IFN-γrel, IL-4/13A and IL-4/13B

Cited by 20 publications

References 32 publications

The Function of Fish Cytokines

The Function of Fish Cytokines

Evolution of Interferons and Interferon Receptors

Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity

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