Induction of vitellogenin synthesis in immature male yellowfin seabream (Acanthopagrus latus) exposed to 4-nonylphenol and 17β-estradiol

Abstract

In the present study, the effects of xenoestrogen 4-nonylphenol (4-NP) and 17β-estradiol (E2) on the synthesis of vitellogenin (VTG) in immature male yellowfin seabream (Acanthoparus latus) were assessed to determine the potential use of this species as a biological model for studying endocrine disrupters in the Musa estuary in southern seawaters of Iran. A total number of 104 fish were injected by 10, 50, 100, 150 and 200 µg g(-) (1) week(-) (1) of 4-NP and 2 µg g(-) (1) week(-) (1) of E2, and their blood plasma was obtained for biochemical analysis on days 0, 7 and 14. The hepatosomatic index (HSI) of each fish was also recorded at each sampling time. The results showed that 4-NP significantly induced VTG synthesis in A. latus, which was detected by gel electrophoresis as two major protein bands (∼210 and 191 KDa). The induction of VTG was also determined indirectly by measuring the alkali-labile phosphate, total plasma calcium and protein. All of these VTG indicators significantly increased in the plasma of 4-NP- and E2-treated fish. Meanwhile, a significant dose-dependent increase was observed in HSI, indicating the activation of hepatic VTG production in 4-NP- and E2-injected fish. Furthermore, the plasma alanine aminotransferase and aspartate aminotransferase concentrations significantly increased in 4-NP-treated fish, presumably reflecting a situation of hepatic tissue damage due to 4-NP toxicity. Based on the results, it is suggested that A. latus could be used successfully as a model species for future studies of endocrine disrupting contaminants in the Musa estuary.

The response of thyroid hormones, biochemical and enzymological biomarkers to pyrene exposure in common carp (Cyprinus carpio)

“…The plasma biochemical constituents, thyroid hormones, liver function enzymes and ionoregulatory endpoints could also be used as biomarkers to reflect the toxicity of chemicals as they have been frequently used for the toxicity assessment of other PAHs in fish and other animals (Alonso-Alvarez et al, 2007;Kim et al, 2008;Latif et al, 2014;Maria et al, 2002;Okwu et al, 2014;Osman et al, 2009;Salman, 2011;Tintos et al, 2008;Wegwu and Omeodu, 2010). Plasma biochemical indicators and transaminases have been proved to be suitable stress biomarkers in fish because they are inexpensive, less variable, more highly conserved between species and are easy to measure (Naderi et al, 2015;Senthil Kumaran et al, 2011).…”

Short-term induction of vitellogenesis in the immature male yellowfin seabream (Acanthopagrus latus) exposed to bisphenol A and 17β-estradiol

“…Several studies have indicated that HSI is an unspecific biomarker to indicate the harmful effects of EDCs (Ishibashi et al, 2006;Kumari et al, 2014;Verslycke et al, 2002). It has also been documented that estrogenic EDCs caused increases in HSI by the alternation of liver VTG production in fish (Feifarek, 2015;Naderi et al, 2015). The liver VTG production can lead to hypertrophy of hepatocytes (Arukwe and Goksøyr, 1998;Zha et al, 2008).…”

Nonylphenol Toxicity Evaluation and Discovery of Biomarkers in Rat Urine by a Metabolomics Strategy through HPLC-QTOF-MS

“…According to an investigation, the structure of NP is similar to 17β-estradiol (E2), and NP can compete to inhibit the combination of E2 with the estrogen receptor (ER) of the target organ [ 44 , 45 ]. It has been reported that E2 could increase the 5-HT level in the nervous system by inhibiting the MAO activity [ 8 , 46 ].…”