Induction of Stress Shock Proteins DnaK and GroEL by Phenoxyherbicide 2,4-D in Burkholderia sp. YK-2 Isolated from Rice Field

Cho, Yun-Seok; Park, Sang-Ho; Kim, Chi-Kyung; Oh, Keun-Yeob

doi:10.1007/s002840010087

Cited by 34 publications

(20 citation statements)

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“…[9], and Burkholderia sp. [10] that are able to metabolize these compounds. In the work described in this paper we used 2D-PAGE to investigate proteins induced in Acinetobacter calcoaceticus in response to phenol and catechol.…”

Section: Introductionmentioning

confidence: 99%

Protein synthesis patterns in Acinetobacter calcoaceticus induced by phenol and catechol show specificities of responses to chemostress

Benndorf¹

2001

FEMS Microbiology Letters

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The proteins induced in Acinetobacter calcoaceticus by the potentially toxic growth substrates phenol and catechol were analyzed by 2D-electrophoresis of cell extracts and compared with those induced by heat shock and oxidative stress. Although both aromatic compounds are quite similar, the only difference being that catechol has an additional hydroxyl group, the responses obtained differed considerably. Phenol has greater lipophilicity and mainly induced heat shock proteins, whereas catechol, which causes the production of reactive oxygen species, predominantly induced oxidative stress proteins. Furthermore, some special proteins were induced by phenol or catechol, which might be useful as biomarkers for chemostress, and could be involved in the catalytic degradation of potentially toxic compounds. ß 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

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Section: Introductionmentioning

confidence: 99%

Protein synthesis patterns in Acinetobacter calcoaceticus induced by phenol and catechol show specificities of responses to chemostress

Benndorf¹

2001

FEMS Microbiology Letters

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“…Oxidative stress may originate from the dioxygenases' activity, which can produce reactive oxygen species (5,36). Four proteins that were more abundant during growth on the aromatic compounds have been linked to oxidative stress responses: the general stress protein GroEL (5,6,15); HslU (25), which degrades abnormal proteins; and AhpCF (4,21,31), which detoxifies peroxides; and a serine kinase (38). Moreover, the increases in purine nucleotide metabolism (PurF and 5,10-methylene-THF reductase) may be an indirect response to oxidative damage.…”

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confidence: 99%

Growth Substrate- and Phase-Specific Expression of Biphenyl, Benzoate, and C ₁ Metabolic Pathways in Burkholderia xenovorans LB400

et al. 2005

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Recent microarray experiments suggested that Burkholderia xenovorans LB400, a potent polychlorinated biphenyl (PCB)-degrading bacterium, utilizes up to three apparently redundant benzoate pathways and a C 1 metabolic pathway during biphenyl and benzoate metabolism. To better characterize the roles of these pathways, we performed quantitative proteome profiling of cells grown on succinate, benzoate, or biphenyl and harvested during either mid-logarithmic growth or the transition between the logarithmic and stationary growth phases. The Bph enzymes, catabolizing biphenyl, were ϳ16-fold more abundant in biphenyl-versus succinate-grown cells. Moreover, the upper and lower bph pathways were independently regulated. Expression of each benzoate pathway depended on growth substrate and phase. Proteins specifying catabolism via benzoate dihydroxylation and catechol ortho-cleavage (ben-cat pathway) were approximately an order of magnitude more abundant in benzoate-versus biphenyl-grown cells at the same growth phase. The chromosomal copy of the benzoyl-coenzyme A (CoA) (box C ) pathway was also expressed during growth on biphenyl: Box C proteins were approximately twice as abundant as Ben and Cat proteins under these conditions. By contrast, proteins of the megaplasmid copy of the benzoyl-CoA (box M ) pathway were only detected in transition-phase benzoate-grown cells. Other proteins detected at increased levels in benzoate-and biphenyl-grown cells included general stress response proteins potentially induced by reactive oxygen species formed during aerobic aromatic catabolism. Finally, C 1 metabolic enzymes were present in biphenyl-grown cells during transition phase. This study provides insights into the physiological roles and integration of apparently redundant catabolic pathways in large-genome bacteria and establishes a basis for investigating the PCB-degrading abilities of this strain.

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“…DnaK and GroEL are induced by 2,4-dichlorophenoxyacetic acid (2,4-D) in Burkholderia sp. YK-2 (Cho et al 2000) and by 4-chlorobiphenyl and biphenyl in B. xenovorans LB400 ). Lacerda et al (2007) investigated the response of a natural community in a continuous-flow wastewater treatment bioreactor to an inhibitory level of cadmium by 2-D PAGE combined with MALDI-TOF/TOF-MS and de novo sequencing.…”

Section: Environmental Stress Responsesmentioning

confidence: 99%

Biodegradation: gaining insight through proteomics

Chauhan

Jain

2010

Biodegradation

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Biodegradation, a generic term used to describe methodologies to affect cleanup of environmental pollutants, has come up as an improved substitute for ineffective and expensive physico-chemical remediation methods. However, lack of information about the factors controlling the growth and metabolism of microorganisms in the polluted environment often limits its implementation. Recent advances in the understanding of biogeochemical processes and genomics have opened up new perspectives towards new opportunities of pollution abatement. High throughput genomic techniques have revolutionized the remediation process leading to breakthroughs in characterizing proteomes, metabolomes and phenotypes for organisms, communities and populations. These new techniques have allowed us to address longstanding questions regarding the molecular mechanisms that may control the mineralization processes and have an in-depth understanding of microbial community structure and stress responses. In order to explore insights of biodegradation this article discusses ways in which proteomics may be able to meet challenges in biodegradation.

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Induction of Stress Shock Proteins DnaK and GroEL by Phenoxyherbicide 2,4-D in Burkholderia sp. YK-2 Isolated from Rice Field

Cited by 34 publications

References 18 publications

Protein synthesis patterns in Acinetobacter calcoaceticus induced by phenol and catechol show specificities of responses to chemostress

Protein synthesis patterns in Acinetobacter calcoaceticus induced by phenol and catechol show specificities of responses to chemostress

Growth Substrate- and Phase-Specific Expression of Biphenyl, Benzoate, and C ₁ Metabolic Pathways in Burkholderia xenovorans LB400

Biodegradation: gaining insight through proteomics

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Induction of Stress Shock Proteins DnaK and GroEL by Phenoxyherbicide 2,4-D in Burkholderia sp. YK-2 Isolated from Rice Field

Cited by 34 publications

References 18 publications

Protein synthesis patterns in Acinetobacter calcoaceticus induced by phenol and catechol show specificities of responses to chemostress

Protein synthesis patterns in Acinetobacter calcoaceticus induced by phenol and catechol show specificities of responses to chemostress

Growth Substrate- and Phase-Specific Expression of Biphenyl, Benzoate, and C 1 Metabolic Pathways in Burkholderia xenovorans LB400

Biodegradation: gaining insight through proteomics

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Growth Substrate- and Phase-Specific Expression of Biphenyl, Benzoate, and C ₁ Metabolic Pathways in Burkholderia xenovorans LB400