Biodegradation: gaining insight through proteomics

Chauhan, Archana; Jain, Rakesh

doi:10.1007/s10532-010-9361-0

Cited by 27 publications

(10 citation statements)

References 157 publications

(151 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…While proteomics approaches have an essential role to play in determining changes in microbial function expression under environmental influences (Bastida et al, 2009;Chauhan and Jain, 2010), little work has been done on them. To our knowledge this is the first study focusing on laccase sequencing from proteins produced in litter.…”

Section: Discussionmentioning

confidence: 99%

Identification of various laccases induced by anthracene and contribution to its degradation in a Mediterranean coastal pine litter

Qasemian¹,

Guiral²,

Belghazi³

et al. 2011

Chemosphere

View full text Add to dashboard Cite

Mediterranean coastal ecosystems are known to be highly subject to natural and anthropic environmental stress. In this study, we examine the effects of anthracene as a common pollutant on the total microbial communities from a Pinus halepensis litter of a typical Mediterranean coastal site (Les Calanques, Marseille). The main objective was to identify the microbial factors leading the resilience of this ecosystem. Two questions were addressed: (i) how lignin-degrading enzymes (Laccase, Lignin-peroxidase and Mn-peroxidase) are affected by the presence of this molecule, (ii) whether the indigenous consortia are involved in its degradation in mesocosms under favorable incubation conditions (25 °C, 60% WHC) and after different time intervals (1 and 3 month(s)). We found a strong increase in laccase production in the presence of anthracene after 3 months, together with anthracene degradation (28%±5). Moreover 9,10-anthraquinone is detected as the product of anthracene oxidation after 3 months. However neither lignin-peroxidase activity nor Mn-peroxidase activity is detected. Laccase proteins directly extracted from litter were sequenced via Nano-LC-MS/MS and reveal twelve different peptide sequences induced by the presence of anthracene in the mesocoms. Our study confirms the major detoxification role of this enzymatic system and highlights the high degradation potential of fungal species inhabiting P. halepensis litter, a factor in the resilience of Mediterranean ecosystems.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of various laccases induced by anthracene and contribution to its degradation in a Mediterranean coastal pine litter

Qasemian¹,

Guiral²,

Belghazi³

et al. 2011

Chemosphere

View full text Add to dashboard Cite

show abstract

“…It is a cheaper method which uses harmless microbial consortium to degrade the pollutants, especially useful for saturated soil and groundwater remediation. The technique includes conditions such as the infiltration of water containing nutrients and oxygen as electron acceptors (Vidali 2001;Chauhan and Jain 2010;Rayu et al 2012).…”

Section: In Situ Bioremediationmentioning

confidence: 99%

“…However, lack of information about the growth and metabolism of microorganisms in the polluted environment often limits its implementation. Recent advances in the understanding of biogeochemical processes and genomics have opened up new perspectives toward new opportunities of pollution abatement (Chauhan and Jain 2010;Jeffries et al 2012;Rayu et al 2012;Tyagi et al 2011). In order for considerable aerobic biodegradation to occur, however, sufficient amounts of dissolved oxygen must exist within the subsurface to serve as an electron acceptor (Adams and Reddy 2003).…”

Section: Biodegradationmentioning

confidence: 99%

“…Bioinformatics, based on proteomics and genomics (Chauhan and Jain 2010;Poirier et al 2013), offers remarkable promise as tools to address longstanding questions regarding the molecular mechanisms involved in the control of mineralization pathways (Kim and Park 2013;Govarthanan et al 2013;Achal et al 2012b). Using a proteomics approach, the physiological changes in an organism during bioremediation provide further insight into bioremediation related genes and their regulation.…”

Section: Application Of Bioremediationmentioning

confidence: 99%

See 1 more Smart Citation

Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation

Mani

Kumar

2013

Int. J. Environ. Sci. Technol.

499

137

View full text Add to dashboard Cite

The ability of heavy metals bioaccumulation to cause toxicity in biological systems-human, animals, microorganisms and plants-is an important issue for environmental health and safety. Recent biotechnological approaches for bioremediation include biomineralization (mineral synthesis by living organisms or biomaterials), biosorption (dead microbial and renewable agricultural biomass), phytostabilization (immobilization in plant roots), hyperaccumulation (exceptional metal concentration in plant shoots), dendroremediation (growing trees in polluted soils), biostimulation (stimulating living microbial population), rhizoremediation (plant and microbe), mycoremediation (stimulating living fungi/mycelial ultrafiltration), cyanoremediation (stimulating algal mass for remediation) and genoremediation (stimulating gene for remediation process). The adequate restoration of the environment requires cooperation, integration and assimilation of such biotechnological advances along with traditional and ethical wisdom to unravel the mystery of nature in the emerging field of bioremediation. This review highlights better understanding of the problems associated with the toxicity of heavy metals to the contaminated ecosystems and their viable, sustainable and eco-friendly bioremediation technologies, especially the mechanisms of phytoremediation of heavy metals along with some case studies in India and abroad. However, the challenges (biosafety assessment and genetic pollution) involved in adopting the new initiatives for cleaning-up the heavy metals-contaminated ecosystems from both ecological and greener point of view must not be ignored.

show abstract

“…All of these three methods when combined reveal a greater understanding of cellular function in an environment [84,85]. Proteomics is being successfully used for identification of novel metabolites that bacteria produce when undergoing a stress response.…”

Section: Desired Community and Its Performancementioning

confidence: 99%

Integrated Perspective for Effective Bioremediation

Paliwal

Puranik

Purohit

2011

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Identification of factors which can influence the natural attenuation process with available microbial genetic capacities can support the bioremediation which has been viewed as the safest procedure to combat with anthropogenic compounds in ecosystems. With the advent of molecular techniques, assimilatory capacity of an ecosystem can be defined with changing community dynamics, and if required, the essential genetic potential can be met through bioaugmentation. At the same time, intensification of microbial processes with nutrient balancing, expressing and enhancing the degradative capacities, could reduce the time frame of restoration of the ecosystem. The new concept of ecosystems biology has added greatly to conceptualize the networking of the evolving microbiota of the niche that helps in effective application of bioremediation tools to manage pollutants as additional carbon source.

show abstract

Biodegradation: gaining insight through proteomics

Cited by 27 publications

References 157 publications

Identification of various laccases induced by anthracene and contribution to its degradation in a Mediterranean coastal pine litter

Identification of various laccases induced by anthracene and contribution to its degradation in a Mediterranean coastal pine litter

Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation

Integrated Perspective for Effective Bioremediation

Contact Info

Product

Resources

About