Induction of stilbene phytoalexins in grapevine (Vitis vinifera) and transgenic stilbene synthase-apple plants (Malus domestica) by a culture filtrate of Aureobasidium pullulans

Rühmann, Susanne; Pfeiffer, Judith; Brunner, Philipp; Szankowski, I.; Fischer, Thilo C.; Forkmann, G.; Treutter, D.

doi:10.1016/j.plaphy.2013.03.011

Cited by 22 publications

(7 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was reported also in experiments reaching high content of resveratrol by the biotransformation of piceid with cellulases in P. cupsidatum [59]. A similar production of trans-resveratrol (about 200 µg/g of DW) has been reported in Vitis cell cultures elicited by centrifugate of the fungus Aerobasidium pullulans [50].…”

Section: Discussionsupporting

confidence: 80%

“…However, fungi also contain another oligosaccharides, proteins and polyunsaturated fatty acids that can trigger the self-defense hypersensitivity reaction and elicit production of phytoalexins [48]. Another defined or complex biotic elicitors used for production of secondary metabolites in grapevine callus and shoot cultures were filtrates, centrifugates, or isolated polysaccharides from yeast, algae and plants [18,49,50].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Sák

Dokupilová²,

Kaňuková

et al. 2021

Plants

View full text Add to dashboard Cite

The in vitro cell cultures derived from the grapevine (Vitis vinifera L.) have been used for the production of stilbenes treated with different biotic and abiotic elicitors. The red-grape cultivar Váh has been elicited by natural cellulose from Trichoderma viride, the cell wall homogenate from Fusarium oxysporum and synthetic jasmonates. The sodium-orthovanadate, known as an inhibitor of hypersensitive necrotic response in treated plant cells able to enhance production and release of secondary metabolite into the cultivation medium, was used as an abiotic elicitor. Growth of cells and the content of phenolic compounds trans-resveratrol, trans-piceid, δ-viniferin, and ɛ-viniferin, were analyzed in grapevine cells treated by individual elicitors. The highest accumulation of analyzed individual stilbenes, except of trans-piceid has been observed after treatment with the cell wall homogenate from F. oxysporum. Maximum production of trans-resveratrol, δ- and ɛ-viniferins was triggered by treatment with cellulase from T. viride. The accumulation of trans-piceid in cell cultures elicited by this cellulase revealed exactly the opposite effect, with almost three times higher production of trans-resveratrol than that of trans-piceid. This study suggested that both used fungal elicitors can enhance production more effectively than commonly used jasmonates.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Sák

Dokupilová²,

Kaňuková

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…Despite limited knowledge of the composition and mechanism of action of yeast and fungal elicitors, autoclaved solutions of yeast culture, fungi mycelia or spores providing cell wall fragments are widely used as elicitors to enhance PSM production, mainly in plant cell or hairy root cultures. An extract of the yeast Aureobasidium pullulans stimulated the accumulation of stilbene phytoalexins in V. vinifera calli [103]. Treatment of P. ginseng cell suspension and hairy root cultures with a yeast preparation enhanced the content of saponins and other unknown compounds [28,80].…”

Section: Biotic Elicitorsmentioning

confidence: 99%

Elicitation, an Effective Strategy for the Biotechnological Production of Bioactive High-Added Value Compounds in Plant Cell Factories

et al. 2016

View full text Add to dashboard Cite

Plant in vitro cultures represent an attractive and cost-effective alternative to classical approaches to plant secondary metabolite (PSM) production (the "Plant Cell Factory" concept). Among other advantages, they constitute the only sustainable and eco-friendly system to obtain complex chemical structures biosynthesized by rare or endangered plant species that resist domestication. For successful results, the biotechnological production of PSM requires an optimized system, for which elicitation has proved one of the most effective strategies. In plant cell cultures, an elicitor can be defined as a compound introduced in small concentrations to a living system to promote the biosynthesis of the target metabolite. Traditionally, elicitors have been classified in two types, abiotic or biotic, according to their chemical nature and exogenous or endogenous origin, and notably include yeast extract, methyl jasmonate, salicylic acid, vanadyl sulphate and chitosan. In this review, we summarize the enhancing effects of elicitors on the production of high-added value plant compounds such as taxanes, ginsenosides, aryltetralin lignans and other types of polyphenols, focusing particularly on the use of a new generation of elicitors such as coronatine and cyclodextrins.

show abstract

“…Accumulation of stilbenic phytoalexins such as trans ‐resveratrol (3,5,4′‐tryhydroxystilbene), its oligomer trans ‐ε‐viniferin, and its derivative trans ‐piceid (5,4′‐dihydroxystilbene‐3‐ O ‐β‐glucopyranoside) have been shown in response to a wide range of plant pathogens (Laquitaine et al , 2006). Furthermore, several studies have indicated that effectiveness of beneficial bacteria for induction of systemic resistance in grapevine against plant pathogens is related to phytoalexin production (Verhagen et al , 2010, 2011; Rühmann et al , 2013; Aziz et al , 2016).…”

Section: Introductionmentioning

confidence: 99%

Induction of systemic resistance to Agrobacterium tumefaciens by endophytic bacteria in grapevine

et al. 2020

View full text Add to dashboard Cite

Crown gall disease of grapevine, caused by Agrobacterium tumefaciens, often results in severe economic loss to grape production worldwide. This study demonstrated the ability of the endophytic bacteria Pseudomonas sp. Sn48 and Pantoea sp. Sa14 isolated from domesticated and wild grapevines to induce resistance in both above‐ and belowground tissues of grapevines infected with A. tumefaciens. Our results provide evidence that both strains can colonize roots and/or shoots. We showed that the strains Pseudomonas sp. Sn48 and Pantoea sp. Sa14 are capable of inducing stilbenic phytoalexin production in grapevine tissues and to further prime plantlets for enhanced phytoalexin production after A. tumefaciens inoculation. We also showed that in the majority of treatments, polyamine accumulation remained unchanged or slightly increased in plantlets treated with Pseudomonas sp. Sn48 and Pantoea sp. Sa14 compared with the control. Our findings indicated that the levels of polyamines remain unchanged or significantly decrease in plantlets treated with endophytic bacteria after A. tumefaciens challenge compared to the control and plantlets treated with individual endophytic bacterial strains. PR1, PR2, and PR4 gene expression levels of plantlets treated with Pseudomonas sp. Sn48 and Pantoea sp. Sa14 significantly increased after A. tumefaciens inoculation. The findings revealed the efficacy of the selected endophytic bacteria in triggering grapevine resistance against A. tumefaciens and the possible use of these strains as an alternative to chemical control methods in grapevine crown gall disease management.

show abstract

Induction of stilbene phytoalexins in grapevine (Vitis vinifera) and transgenic stilbene synthase-apple plants (Malus domestica) by a culture filtrate of Aureobasidium pullulans

Cited by 22 publications

References 54 publications

Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Biotic and Abiotic Elicitors of Stilbenes Production in Vitis vinifera L. Cell Culture

Elicitation, an Effective Strategy for the Biotechnological Production of Bioactive High-Added Value Compounds in Plant Cell Factories

Induction of systemic resistance to Agrobacterium tumefaciens by endophytic bacteria in grapevine

Contact Info

Product

Resources

About