Induction of sister-chromatid exchanges by procarcinogens in metabolically competent Chinese hamster epithelial liver cells

Salvia, R. De; Meschini, Roberta; Fiore, Mario; Polani, Stefania; Palitti, F.; Carluccio, Maria Annunziata; Turchi, G.

doi:10.1016/0165-7992(88)90044-9

Cited by 14 publications

(3 citation statements)

References 25 publications

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“…There is complete agreement between our results and other findings of analogous experiments in CHO cells with an external metabolizing source (S9), in which phenolphthalein induced chromosomal aberrations only in the presence of the liver fraction S9 [4]. The validity of CHEL cells to activate different inactive substances in compounds having clastogenic or mutagenic activity is once again confirmed [16][17][18]. A similar positive response on chromosome aberration induction was obtained when human cells from AF were treated with phenolphthalein (Table II).…”

Section: Discussionsupporting

confidence: 81%

“…In this context, to better investigate the cytogenetic effects of phenolphthalein we also assayed this chemical for chromosome aberration in a Chinese hamster cell line, CHEL cells (Chinese hamster epithelial liver cells) metabolically competent to activate different classes of procarcinogens and promutagens into biologically active metabolites [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Phenolphthalein induces chromosome aberrations in human and Chinese hamster liver cells (CHEL) cultured in vitro

Biondi

Andreozzi

Amoruso

et al. 2000

Teratog. Carcinog. Mutagen.

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Phenolphthalein is a nonprescription laxative agent that has been widely used during this century. Recent studies in animal models have shown that phenolphthalein has carcinogenic activity. In order to assess cytogenetic effects on human cells in vitro, we tested phenolphthalein in a chromosome aberration assay in human embryo cells derived from amniotic fluid. Our results show that phenolphthalein induces a significant increase in the frequency of chromosome aberrations in human cells. The lowest dose level at which the clastogenic effect is evident is 23.2 microg/ml. Similar positive results were obtained in a Chinese hamster liver cell line, which is metabolically competent to activate different classes of promutagens and procarcinogens into biologically active metabolites. Instead, parallel experiments in Chinese hamster ovary cells did not show any clastogenic effect due to phenolphthalein. These latter data suggested that phenolphthalein acts as a promutagen and must be metabolically activated to exert its clastogenic effect. Teratogenesis Carcinog. Mutagen. 20:209-217, 2000.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Phenolphthalein induces chromosome aberrations in human and Chinese hamster liver cells (CHEL) cultured in vitro

Biondi

Andreozzi

Amoruso

et al. 2000

Teratog. Carcinog. Mutagen.

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“…Fluoranthene and pyrene could induce SCE in CHO-K1 cells (Roggeband et al 1994). Pyrene and phenanthrene could also induce chromosomal aberration (CA) in Chinese hamster V79-4 cells without metabolic activation (Yan et al 2004), while benzo(a) pyrene could induce CA without metabolic activation in rat liver RL1 cells (Pal 1981) and in mouse lymphoma L5178 Y cells (Baird et al 1984), MN and SCE in human hepatoma cells, SCE in rat pleural mesothelioma cells (Abe et al 1983), Chinese hamster V 79 cells (Dean 1981), human hepatoma cells (De Salvia et al 1988), Chinese hamster epithelial liver cells (Yan et al 2004), and in CHO cells (Roggeband et al 1994). Benzo(a) pyrene could also induce CA with and without metabolic activation in human fibroblasts WI-38 cells (Fernandez and Haridon 1994) and in human lymphocytes (USEPA 1980).…”

Section: )mentioning

confidence: 98%

The Genotoxicity of Priority Polycyclic Aromatic Hydrocarbons (PAHs) Containing Sludge Samples

Krishnamurthi

Devi

Chakrabarti

2007

Toxicology Mechanisms and Methods

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In this research work we developed in vitro tests utilizing mammalian cell cultures, which can rapidly assess effect of exposure of oily sludge-derived chemicals on human and ecological health. Many of these are hazardous to health and environment due to their toxicity and/or accumulation potential in sediments as well as in organisms. Petroleum refinery and petrochemical industry-derived oily sludges contain toxic polycyclic aromatic hydrocarbons (PAHs), some of which are lipophilic in nature. Risk assessment of environmental samples suffers from inadequate availability of toxicity data, lack of knowledge about behavior of genotoxic substances in complex matrices, paucity of information on synergistic and antagonistic interactions of mixture of components, etc.; the literature describing the behavior of genotoxic substances in complex mixtures is sparse and sometimes contradictory. The present study aims at assessing the genotoxic potential of oily sludges collected from an integrated petroleum refinery and petrochemical industry located in the southwestern part of India and a petrochemical industry located in the western part of India using a battery of genotoxicity assays such as DNA damage/strand break, chromosomal aberration, p(53) protein induction, and apoptosis in CHO-K1 cell culture system. Exposure with different dose levels of sludge extracts (25, 50, 100 muL) in CHO-K1 cells could cause statistically significant level of (P < 0.001) DNA damage, chromosomal aberration, p(53) protein induction, and apoptosis in comparison to negative control treatment groups, and the genotoxicity was attributed to PAHs present in the sludge as identified by GC-MS. This implies that the sludges are genotoxic in nature in mammalian cells tested, and the exposure to these may pose a potential genotoxic risk to human beings.

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Clastogenic effects of the dithiocarbamate fungicides thiram and ziram in Chinese hamster cell lines cultured in vitro

Mosesso

Turchi

Cinelli³

et al. 1994

Teratog Carcinog Mutagen

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We report here the results obtained using the dithiocarbamate fungicides thiram and ziram to investigate the induction of chromosomal aberrations (CAs) in Chinese hamster ovary (CHO) cells both in the absence and presence of S9 metabolism, and in a Chinese hamster epithelial liver (CHEL) cells which retain metabolic competence to activate different classes of promutagens/procarcinogens. Both thiram and ziram proved to be strong chromosome breaking agents in the CHEL cells and CHO cells in the presence of S9 metabolism. These findings suggest that thiram and ziram require metabolic conversion to become genetically active, and corroborate the evidence that CHEL cells are suitable to activate and detect a broad spectrum of chemical procarcinogens including these two pesticides.

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Induction of sister-chromatid exchanges by procarcinogens in metabolically competent Chinese hamster epithelial liver cells

Cited by 14 publications

References 25 publications

Phenolphthalein induces chromosome aberrations in human and Chinese hamster liver cells (CHEL) cultured in vitro

Phenolphthalein induces chromosome aberrations in human and Chinese hamster liver cells (CHEL) cultured in vitro

The Genotoxicity of Priority Polycyclic Aromatic Hydrocarbons (PAHs) Containing Sludge Samples

Clastogenic effects of the dithiocarbamate fungicides thiram and ziram in Chinese hamster cell lines cultured in vitro

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