Clastogenic effects of the dithiocarbamate fungicides thiram and ziram in Chinese hamster cell lines cultured in vitro

Mosesso, Pasquale; Turchi, G.; Cinelli, Serena; Chiara, D. Di; Fiore, Mario; Palitti, F.

doi:10.1002/tcm.1770140306

Cited by 20 publications

(3 citation statements)

References 32 publications

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“…There is complete agreement between our results and other findings of analogous experiments in CHO cells with an external metabolizing source (S9), in which phenolphthalein induced chromosomal aberrations only in the presence of the liver fraction S9 [4]. The validity of CHEL cells to activate different inactive substances in compounds having clastogenic or mutagenic activity is once again confirmed [16][17][18]. A similar positive response on chromosome aberration induction was obtained when human cells from AF were treated with phenolphthalein (Table II).…”

Section: Discussionsupporting

confidence: 81%

“…In this context, to better investigate the cytogenetic effects of phenolphthalein we also assayed this chemical for chromosome aberration in a Chinese hamster cell line, CHEL cells (Chinese hamster epithelial liver cells) metabolically competent to activate different classes of procarcinogens and promutagens into biologically active metabolites [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

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Phenolphthalein induces chromosome aberrations in human and Chinese hamster liver cells (CHEL) cultured in vitro

Biondi

Andreozzi

Amoruso

et al. 2000

Teratog. Carcinog. Mutagen.

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Phenolphthalein is a nonprescription laxative agent that has been widely used during this century. Recent studies in animal models have shown that phenolphthalein has carcinogenic activity. In order to assess cytogenetic effects on human cells in vitro, we tested phenolphthalein in a chromosome aberration assay in human embryo cells derived from amniotic fluid. Our results show that phenolphthalein induces a significant increase in the frequency of chromosome aberrations in human cells. The lowest dose level at which the clastogenic effect is evident is 23.2 microg/ml. Similar positive results were obtained in a Chinese hamster liver cell line, which is metabolically competent to activate different classes of promutagens and procarcinogens into biologically active metabolites. Instead, parallel experiments in Chinese hamster ovary cells did not show any clastogenic effect due to phenolphthalein. These latter data suggested that phenolphthalein acts as a promutagen and must be metabolically activated to exert its clastogenic effect. Teratogenesis Carcinog. Mutagen. 20:209-217, 2000.

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Section: Discussionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Phenolphthalein induces chromosome aberrations in human and Chinese hamster liver cells (CHEL) cultured in vitro

Biondi

Andreozzi

Amoruso

et al. 2000

Teratog. Carcinog. Mutagen.

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“…Thiram was found to induce malsegregation in fungi (Upshall and Johnson, 1981), excisable DNA lesions in bacteria (Crebelli et al, 1992), and also apoptosis in neuronal-like pheochromocytoma cells (Sook Han et al, 2003), chick chondrocytes (Rath et al, 1995), and Chinese hamster cells (Grosicka et al, 2005). Other examples of the consequences determined by treating Chinese hamster cells with Thiram are point mutations at the hypoxanthine-guanine phosphoribosyl transferase locus (Paschin and Bakhitova, 1985), increased CAs (Mosesso et al, 1994), cellular reduced glutathione (GSH) depletion, and protein and membrane lipid peroxidation (LPO) (Grosicka et al, 2005). In mice, the fungicide was associated with the formation of micronuclei in the bone marrow (Paschin and Bakhitova, 1985), but not in polychromatic erythrocytes (George and Kuttan, 1995) or splenocytes (Villani et al, 1998).…”

Section: Introductionmentioning

confidence: 95%

Chromosomal aberrations in cultured human lymphocytes treated with the fungicide, Thiram

Santovito¹,

Cervella²,

Delpero³

2012

Drug and Chemical Toxicology

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In vitro effects of different concentrations of Thiram were tested on human lymphocytes to determine, by means of the chromosome aberrations (CAs) assay, whether this fungicide could induce clastogenic damage. Evidences of the effect of Thiram on human lymphocytes were limited to sister chromatid exchange, micronuclei formation, and comet assays. We evaluated 0.01, 0.1, 1.2, and 12.0 μg/mL of Thiram, where 0.01 μg/mL represent the acceptable daily intake dose set by the World Health Organization and the Food and Agriculture Organization for fruit and vegetables, whereas 0.1, 1.2, and 12.0 μg/mL are its multiple values. Results indicated that human lymphocytes treated in vitro with Thiram at concentrations of 1.20 and 12.0 μg/mL significantly increased CAs frequency, compared with the negative control, whereas at lower concentrations (0.01 and 0.1 μg/mL), this effect was not observed. However, Thiram showed a clastogenic effect also at the concentration value of 1.2 μg/mL that represents a lower value with respect to the residue limits found in Italy for grapes, strawberries, potatoes, tobacco, and other fruits and vegetables. Finally, according to some evidence obtained from the study of other fungicides, Thiram produced a significant reduction in the mitotic index with increasing concentration.

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Thiram [MAK Value Documentation in German language, 2007]

2012

The MAK‐Collection for Occupational Health and Safety

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Veröffentlicht in der Reihe Gesundheitsschädliche Arbeitsstoffe , 43. Lieferung, Ausgabe 2007 Der Artikel enthält folgende Kapitel: Allgemeiner Wirkungscharakter Wirkungsmechanismus Toxikokinetik und Metabolismus Erfahrungen beim Menschen Subakute, subchronische und chronische Toxizität Allergene Wirkung Reproduktionstoxizität Tierexperimentelle Befunde und In‐vitro‐Untersuchungen Akute Toxizität Subakute, subchronische und chronische Toxizität Wirkung auf Haut und Schleimhäute Allergene Wirkung Reproduktionstoxizität Kanzerogenität Bewertung

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Clastogenic effects of the dithiocarbamate fungicides thiram and ziram in Chinese hamster cell lines cultured in vitro

Cited by 20 publications

References 32 publications

Phenolphthalein induces chromosome aberrations in human and Chinese hamster liver cells (CHEL) cultured in vitro

Phenolphthalein induces chromosome aberrations in human and Chinese hamster liver cells (CHEL) cultured in vitro

Chromosomal aberrations in cultured human lymphocytes treated with the fungicide, Thiram

Thiram [MAK Value Documentation in German language, 2007]

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