Induction of protective immune responses in mice by double DNA vaccine encoding of &lt;i&gt;Brucella melitensis&lt;/i&gt; Omp31 and &lt;i&gt;Escherichia coli&lt;/i&gt; Eae genes

Ranjbar, Reza; Sharifimoghadam, Samanehsadat; Saeidi, Elnaz; Jonaidi, Nematollah; Sheikhshahrokh, Amirhossein

doi:10.4314/tjpr.v15i10.4

Cited by 3 publications

(3 citation statements)

References 19 publications

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“…Plasmid DNA carrying the BLS gene was additionally a good candidate for vaccination against Brucella (17). Another previous study demonstrated a protective immune response induced by a novel double DNA vaccine encoding the Brucella melitensis omp31 gene and the E. coli eae gene in a mouse model (31). All these results suggested that DNA vaccines demonstrated great immunogenicity and protective efficacy against infection in a mouse model.…”

Section: Discussionmentioning

confidence: 87%

Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice

et al. 2018

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Brucellosis is an important neglected zoonotic disease, and the pathogens responsible are Brucellae. In order to evaluate the immunogenicity and protective efficacy of a DNA vaccine encoding Brucella BvrR, the recombinant plasmid pCDNA-BvrR was constructed by inserting the BvrR gene fragment into a pCDNA3.0 vector. The His 6-tagged BvrR was purified with His-trap FF crude affinity chromatography and verified with an anti-histidine monoclonal antibody by western blot analysis. The specific immunoglobulin antigens and their isotypes were detected by indirect ELISA. The recombinant His 6-BvrR protein was expressed and purified by affinity chromatography. The optical density 450 value of immunoglobulin G (IgG) in the pCDNA-BvrR group was significantly increased compared with the pCDNA3.0 vector or PBS groups (P<0.05), and the pCDNA3.0 vector and PBS groups exhibited no significant difference (P>0.05). BvrR induced specific antibodies with a dominance of IgG2a over IgG1 and the T cell-proliferative response, in addition to a typical T helper-1 (Th1)-dominated immune response in mice. The splenocytes from mice of the pCDNA-BvrR group demonstrated significant proliferative activity compared with the pCDNA3.0 vector group. The present results indicated that immunization with BvrR induced a specific Th1-type immune response in mice. Subsequent to challenging with B. abortus S19, it was identified that the DNA vaccine pCDNA-BvrR induced a significant level of protection in BALB/c mice by evaluating systemic bacterial clearance. These results suggested that BvrR may be a good candidate for a DNA vaccine against brucellosis.

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Section: Discussionmentioning

confidence: 87%

Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice

et al. 2018

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“…Omp31 and Omp25B are immunogenic and were reported to induce humoral and cellular immune responses, as well as have protective effects against Brucella (Clausse et al, 2013 ). A study reported that mice immunized with peptides of OMP31 containing T epitope, B epitope or TB epitopes are of high immunogenicity, which can protect mice from B. melitensis infection in the lung (Zhang et al, 2019 ) and that a stable memory immune response was produced (Ranjbar et al, 2016 ). Therefore, exosomes containing antigens can inhibit the reproduction of Brucella in mice and induce the M1 polarization of macrophages in vivo to promote Th1‐type immunity.…”

Section: Discussionmentioning

confidence: 99%

Exosomes released by Brucella‐infected macrophages inhibit the intracellular survival of Brucella by promoting the polarization of M1 macrophages

Wang

et al. 2023

Microbial Biotechnology

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Exosomes, membrane vesicles released extracellularly from cells, contain nucleic acids, proteins, lipids and other components, allowing the transfer of material information between cells. Recent studies reported the role of exosomes in pathogenic microbial infection and host immune mechanisms. Brucella‐invasive bodies can survive in host cells for a long time and cause chronic infection, which causes tissue damage. Whether exosomes are involved in host anti‐Brucella congenital immune responses has not been reported. Here, we extracted and identified exosomes secreted by Brucella melitensis M5 (Exo‐M5)‐infected macrophages, and performed in vivo and in vitro studies to examine the effects of exosomes carrying antigen on the polarization of macrophages and immune activation. Exo‐M5 promoted the polarization of M1 macrophages, which induced the significant secretion of M1 cytokines (tumour necrosis factor‐α and interferon‐γ) through NF‐κB signalling pathways and inhibited the secretion of M2 cytokines (IL‐10), thereby inhibiting the intracellular survival of Brucella. Exo‐M5 activated innate immunity and promoted the release of IgG2a antibodies that protected mice from Brucella infection and reduced the parasitaemia of Brucella in the spleen. Furthermore, Exo‐M5 contained Brucella antigen components, including Omp31 and OmpA. These results demonstrated that exosomes have an important role in immune responses against Brucella, which might help elucidate the mechanisms of host immunity against Brucella infection and aid the search for Brucella biomarkers and the development of new vaccine candidates.

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“…DNA-based vaccines contain gene sequences of pathogens, which are essential for intracellular survival of Brucella spp. The immunogenicity and efficacy of these virulence genes used in DNA vaccines have been demonstrated in animal studies, including the two-component BvrR/BvrS system ( 119 ), Cu-Zn superoxide dismutase (SOD) ( 126 , 140 ), ribosomal L7/L12 or Brucella lumazine synthase (BLS) ( 139 , 141 ), B. melitensis omp31 and omp25 genes ( 125 , 142 ), antigenic surface protein (BCSP31) gene ( 120 ), SP41 ( 143 ), and ribosomal protein L9 (rL9) ( 122 ). According to the studies that have been done, DNA vaccines may have the ability to resolve the disadvantages of other brucellosis vaccines ( 119 , 120 , 144 ).…”

Section: Dna Vaccinesmentioning

confidence: 99%

Evaluation of Brucellosis Vaccines: A Comprehensive Review

Heidary

Dashtbin

Ghanavati³

et al. 2022

Front. Vet. Sci.

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Brucellosis is a bacterial zoonosis caused by Brucella spp. which can lead to heavy economic losses and severe human diseases. Thus, controlling brucellosis is very important. Due to humans easily gaining brucellosis from animals, animal brucellosis control programs can help the eradication of human brucellosis. There are two popular vaccines against animal brucellosis. Live attenuated Brucella abortus strain 19 (S19 vaccine) is the first effective and most extensively used vaccine for the prevention of brucellosis in cattle. Live attenuated Brucella melitensis strain Rev.1 (Rev.1 vaccine) is the most effective vaccine against caprine and ovine brucellosis. Although these two vaccines provide good immunity for animals against brucellosis, the expense of persistent serological responses is one of the main problems of both vaccines. The advantages and limitations of Brucella vaccines, especially new vaccine candidates, have been less studied. In addition, there is an urgent need for new strategies to control and eradicate this disease. Therefore, this narrative review aims to present an updated overview of the available different types of brucellosis vaccines.

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Induction of protective immune responses in mice by double DNA vaccine encoding of <i>Brucella melitensis</i> Omp31 and <i>Escherichia coli</i> Eae genes

Cited by 3 publications

References 19 publications

Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice

Evaluation of a DNA vaccine encoding Brucella BvrR in BALB/c mice

Exosomes released by Brucella‐infected macrophages inhibit the intracellular survival of Brucella by promoting the polarization of M1 macrophages

Evaluation of Brucellosis Vaccines: A Comprehensive Review

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