Induction of pluripotent stem cells by reprogramming human ocular fibroblasts under xeno-free conditions

Abstract

Sendai virus facilitates induced pluripotent stem cell reprogramming of ocular fibroblasts-both human pterygium and human Tenon's capsule fibroblasts being safe and efficient for induced pluripotent stem cell reprogramming. Although the reprogramming efficiencies of ocular-derived induced pluripotent stem cells under xeno-free conditions were not superior to those observed using the traditional reprogramming system, the cell therapy system reprogramming system is a good option when induced pluripotent stem cells are to be induced under xeno-free conditions.

Carbon Nanotube Polymer Scaffolds as a Conductive Alternative for the Construction of Retinal Sheet Tissue

“…As in previous studies, we have developed an optimized system for derivation of 3D retinal tissue. − Stem-cell-derived retinal organoids are seeded on poly­(lactic- co -glycolic acid) (PLGA) scaffolds and directed toward retinal ganglion cell (RGC) fate. In the present study, we synthesized the CNT-PLGA scaffolds by electrospinning technology and assessed their conductivity, biocompatibility, and degradation.…”

Generation and delivery of “Yamanaka factor” recombinant proteins mediated with magnetic iron oxide nanoparticles (MIONPs)

“…However, the lower reprogramming efficiency and especially potential safety issues such as insertional mutagenesis of genome, unpredictable genetic dysfunction, and tumorigenicity limit the clinical applications of iPSCs. New methodological modifications such as reducing the number of defined factors, using non-integrating virus vector (such as adenovirus) (Ye and Wang 2018), excisable vector (such as piggyBac) (Rodriguez-Polo et al 2019), or non-viral vector (such as transposon system) (Kues et al 2013), establishing xeno-free conditions (Xiong et al 2018), and developing non-DNA inducers such as synthetic mRNA (McGrath et al 2018), self-replicating RNA (srRNA) (Steinle et al 2019), microRNA (miRNA) (Kogut et al 2018), small molecule (Ma et al 2017), or recombinant protein were developed to weaken or overcome these drawbacks. As a novel, nucleic acid-free, and non-integrating strategy, recombinant protein-mediated reprogramming can effectively eliminate any risks of insertional mutagenesis and provide a substantially simpler, faster, and safer approach to generate iPSCs.…”