Induction of Plantlet Regeneration on Shallot (Allium cepa var. Aggregatum)

Abstract

Micropropagation is an alternative method for producing pathogenic contamination-free shallots seedlings. The aim of this research was to determine the optimum balance of auxin-cytokinin and sucrose concentration in stimulating the plantlet regeneration on shallot. The research was conducted from March to August 2019. In this study, the auxin and cytokinin treatment (1-5 ppm) were used in the format of a randomized complete block design in three replications. The basal plate containing a shoot bud was excised from healthy bulb and used as planting material. The explants had been through a sterilization process and was cultured aseptically in medium containing each treatment. Culture medium contained 7 g.L -1 agar powder and were adjusted at pH 5.8 before autoclaving. The multiple shootlet (10 weeks of cultured) were then subcultured for bulblet formation in MS medium supplemeted with BAP dan succrose (3-9%). The basal plate containing a shoot bud was excised from healthy bulb and used as planting material. The explants had been through a sterilization process and was cultured aseptically in medium containing each treatment. Culture medium contained 7 g L -1 agar powder and were adjusted at pH 5.8 before autoclaving. The results showed that the use of ½ MS media could increase the efficiency of using nutrient solution of tissue culture media. Micro shoot formation can be initiated on ½ MS medium with the addition of 1 ppm BAP (1.36 shoots / explants). This media also produced the best response for the number of micro roots (5.63 roots / explants), and the number of micro leaves (3.91 leaves). However, the difference in composition of nutrient in culture media was not able to accelerate the time of growth of shoots, roots and leaves. Further subculture in ½ strength of MS medium containing of BAP and sucrose 6% increased shoot, root and bulblet formation (13.3 shoots, 41.3 roots and 10.7 bulblets).

Pertumbuhan dan Hasil Genotipe Bawang Merah pada Peningkatan Dosis Sulfur melalui Sistem Hidroponik Nutrient Film Technique

“…Keanekaragaman fenotip bawang terletak pada ukuran dan warna umbi, panjang daun, habitat dan sifat agronomis lainnya seperti tanggapan terhadap stress dan mekanisme toleransi (Irawan et al, 2021) . Sifat unggul yang dimiliki bawang merah seperti tahan penyakit, tanaman yang tinggi, jumlah anakan sedang, umur tanaman genjah, ukuran umbi besar, warna umbi merah tua, serta bentuk umbinya bulat adalah tipe bawang merah yang ideal (Marlin et al, 2021).…”