Induction of plant disease resistance upon treatment with yeast cell wall extract

Yaguchi, Takaomi; Kinami, Tomohisa; Ishida, Tetsuya; Yasuhara, Toru; Takahashi, Kosaku; Matsuura, Hideyuki

doi:10.1080/09168451.2017.1379351

Cited by 9 publications

(7 citation statements)

References 24 publications

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“…In addition to PR gene activation, a yeast cell wall extract added to rice cell suspension culture induced accumulation of signaling molecules involved in SAR; azelaic acid (AzA) and phenylalanine (Phe), as well as the wound-based signaling compound JA, and related jasmonate intermediates and actives 12-hydroxyjasmonoyl isoleucine and 12-oxo-phytodienoic acid (Yaguchi et al, 2017). These compounds were proposed to prime against the necrotrophic pathogen Botrytis cinerea in Arabidopsis .…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have been performed to test the efficacy of microbial fermentation products (MFPs) as elicitors of plant defence, including a yeast cell wall extract derived from the beer brewing process (Yaguchi et al, 2017), and a compound derived from glutamate fermentation (Chen et al, 2014). Derivatives of the yeast cell wall, including glucan, mannon, and chitin, have all been investigated as inducers of plant defence (Reglinski et al, 1994; Boller, 1995; Reglinski et al, 1995; Minami et al, 2011; Narusaka et al, 2015; Yaguchi et al, 2017), whereas lactic acid bacteria have been shown to prevent fungal disease in the field (Oliveira et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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A Microbial Fermentation Mixture Primes for Resistance Against Powdery Mildew in Wheat

2019

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Since many fungal pathogens develop resistance to fungicides, novel and low-cost alternative methods to improve plant health and fitness need to be developed. An approach to improve productivity in crops is to stimulate the plant’s own defence mechanisms via priming. Therefore, we investigated if a fermentation-based elicitor could prime plant defences against powdery mildew in wheat by inducing the expression of endogenous defence-related genes. Wheat seedlings were spray-treated with a fermentation-based elicitor 8 days prior to inoculation with powdery mildew. Disease assays showed a significantly reduced number of powdery mildew pustules were formed on wheat treated with the mixed elicitor. In vitro sensitivity assays tested the ability of powdery mildew conidia to germinate on agar amended with the fermentation-based product and concluded that fungal germination and differentiation were also inhibited. Tissue samples were taken at time points pertaining to different developmental stages of powdery mildew infection. Significantly higher expression of PR genes (PR1, PR4, PR5, and PR9) was observed in the microbial fermentation mixture-treated plants compared with untreated plants. These genes are often associated with the elicitation of plant defence responses to specific biotrophic pathogens, such as powdery mildew, suggesting an elicitor-mediated response in the wheat plants tested. The product components were assessed, and the components were found to act synergistically in the microbial fermentation mixture. Therefore, this fermentation-based elicitor provides an effective method for powdery mildew control.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Microbial Fermentation Mixture Primes for Resistance Against Powdery Mildew in Wheat

2019

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“…Plant pattern-recognition receptors that perceive yeast PAMPs remain largely unknown, although many studies demonstrate the ability of yeasts to activate plant immune signaling. For example, yeast cell wall extract of the budding yeast Saccharomyces pastorianus activates JA and azelaic acid signaling to induce plant disease resistance (Yaguchi et al, 2017). In Arabidopsis, yeast cell wall extract activates the JA, ethylene, and SA signaling pathways, and SA accumulation and signaling were required for induced resistance (Narusaka et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Several plants exhibit multiple responses to various yeast elicitor preparations; such as autoclaved baker's yeast (Saccharomyces cerevisiae), preparations from S. cerevisiae cell walls, or commercial yeast extract. These responses include, increased immunity against pathogens, induction of reactive oxygen species accumulation, and activation of known immune signaling pathways, in the model plant Arabidopsis thaliana (referred to hereafter as Arabidopsis; Raacke et al, 2006;Khokon et al, 2010;Minami et al, 2011;Ye et al, 2013;Narusaka et al, 2015;Yaguchi et al, 2017) and other plants (Sun et al, 2018a(Sun et al, , 2018b. Raacke et al (2006) used reverse genetics with Arabidopsis to demonstrate that yeast treatments induced salicylic acid (SA) dependent immunity against Pseudomonas syringae; however, induced resistance against the fungal pathogen Botrytis cinerea (hereafter referred to as Botrytis) was independent of the SA, jasmonate, and camalexin pathways.…”

Section: Introductionmentioning

confidence: 99%

The immunity priming effect of the Arabidopsis phyllosphere resident yeast Protomyces arabidopsidicola strain C29

2022

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The phyllosphere is a complex habitat for diverse microbial communities. Under natural conditions, multiple interactions occur between host plants and phyllosphere resident microbes, such as bacteria, oomycetes, and fungi. Our understanding of plant associated yeasts and yeast-like fungi lags behind other classes of plant-associated microbes, largely due to a lack of yeasts associated with the model plant Arabidopsis, which could be used in experimental model systems. The yeast-like fungal species Protomyces arabidopsidicola was previously isolated from the phyllosphere of healthy wild-growing Arabidopsis, identified, and characterized. Here we explore the interaction of P. arabidopsidicola with Arabidopsis and found P. arabidopsidicola strain C29 was not pathogenic on Arabidopsis, but was able to survive in its phyllosphere environment both in controlled environment chambers in the lab and under natural field conditions. Most importantly, P. arabidopsidicola exhibited an immune priming effect on Arabidopsis, which showed enhanced disease resistance when subsequently infected with the fungal pathogen Botrytis cinerea. Activation of the mitogen-activated protein kinases (MAPK), camalexin, salicylic acid, and jasmonic acid signaling pathways, but not the auxin-signaling pathway, was associated with this priming effect, as evidenced by MAPK3/MAPK6 activation and defense marker expression. These findings demonstrate Arabidopsis immune defense priming by the naturally occurring phyllosphere resident yeast species, P. arabidopsidicola, and contribute to establishing a new interaction system for probing the genetics of Arabidopsis immunity induced by resident yeast-like fungi.

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“…The increase of intermediate, precursors and catalytic enzymes involved in jasmonic acid biosynthetic pathways were reportedly observed following yeast extract elicitation (Yaguchi T et al, 2017;Hasanloo T et al, 2009). Thus, chitosan and yeast extract would bi-laterally enhance the aloe-emodin production through the jasmonic acid-signalling pathway and create a new synergistic elicitor duo.…”

Section: Simultaneous Double Elicitation On Aloe-emodin Productionmentioning

confidence: 96%

Effects of Sequential and Simultaneous Double Elicitation on Aloe-Emodin Production in Cassia Tora Root Cultures

Teptat¹,

Tansakul²,

Sakunphueak³

2020

Preprint

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Abstract Cassia tora root cultures were established to study anthraquinone production and as an alternative source of anthraquinone. In this study, several plant defence-based techniques were used to enhance aloe-emodin production in Cassia tora root cultures. Elicitation and the initial optimisation of exposure time and culture age suggested that 100 mg/L yeast extract was the most efficient elicitor, with the highest efficiency during early to mid-stationary growth phase. The elicited root produced approximately 2.7-2.9 folds-improvement in aloe-emodin content when compared with the control. The efficiency of the elicitor tended to increase along with the exposure time. Extending the elicitation period to the end of culture cycle (9-10 days) increased the production up to 6.21 times (5.740 ± 0.584 mg/gDW). Simultaneous double elicitation and sequential double elicitation also showed their effectiveness on aloe-emodin production. Aloe-emodin production was improved up to 9.09 times higher than the control by a simultaneous add-on of 50 mg/L chitosan with 100 mg/L yeast extract. Meanwhile, sequential elicitation of 50 mg/L chitosan for 24 hours prior to elicitation by yeast extract improved the production up to 11.27 times higher than the control. Both treatment styles exhibited a particular advantage toward the extracellular accumulation, which might facilitate the use of two-phase culture or in conjunction with other enhancing techniques.

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Induction of plant disease resistance upon treatment with yeast cell wall extract

Cited by 9 publications

References 24 publications

A Microbial Fermentation Mixture Primes for Resistance Against Powdery Mildew in Wheat

A Microbial Fermentation Mixture Primes for Resistance Against Powdery Mildew in Wheat

The immunity priming effect of the Arabidopsis phyllosphere resident yeast Protomyces arabidopsidicola strain C29

Effects of Sequential and Simultaneous Double Elicitation on Aloe-Emodin Production in Cassia Tora Root Cultures

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