“…Further curation that integrates additional properties such as vitamin auxotrophies and storage (66), as well as metabolic shifts occurring from gene regulation (67,68), is likely to improve the quantitative predictive power of this framework (33,69). Moreover, although our predictions based on metabolic mechanisms are informative of key aspects underlying the assembly of plantassociated microbiomes, they do not consider additional factors that are known to shape leaf communities, such as signaling molecules, antagonistic interactions, or host immunity (5,(70)(71)(72)(73). A potential consequence of this limitation may be seen in the example of Pseudomonas sp.…”