1996
DOI: 10.1002/(sici)1097-4652(199608)168:2<442::aid-jcp23>3.3.co;2-g
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Induction of normal and psoriatic phenotypes in submerged keratinocyte cultures

Abstract: Lesional psoriatic epidermis displays a number of phenotypic changes that are distinct from the differentiation program found in normal interfollicular epidermis. In psoriatic epidermis, keratinocytes are hyperproliferative and several differentiation-associated molecules are expressed that are absent in normal skin (e.g., cytokeratins (CK) 6, 16, and 17, and the epidermal proteinase inhibitor SKALP/ elafin). In addition, several molecules which are normally restricted to the stratum granulosum are strongly up… Show more

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Cited by 25 publications
(41 citation statements)
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“…CK16 and CK17 tended to be negative in the suprabasal cells of this part, confirming the infundibular differentiation. However, they were positive for the whole areas in some early or proliferative stage lesions, which may suggest either the hyperproliferative nature of this stage of lesions 46 or the beginning of the outer sheath differentiation in the invaginated part.…”
Section: Discussionmentioning
confidence: 93%
“…CK16 and CK17 tended to be negative in the suprabasal cells of this part, confirming the infundibular differentiation. However, they were positive for the whole areas in some early or proliferative stage lesions, which may suggest either the hyperproliferative nature of this stage of lesions 46 or the beginning of the outer sheath differentiation in the invaginated part.…”
Section: Discussionmentioning
confidence: 93%
“…AD, atopic dermatitis; NS, normal skin; PS, psoriasis. (van Ruissen et al, 1996;Zeeuwen et al, 2008). In differentiated keratinocyte cultures that show a normal skin phenotype, dectin-1 mRNA expression levels were low.…”
Section: Protein Expression Of Innate Immunity Genesmentioning
confidence: 98%
“…The evaluation of avarol and derivatives in keratinocyte differentiation models Using our keratinocyte differentiation culture models (van Ruissen et al, 1996;Pol et al, 2002), the effects of avarol and its 14 avarol derivatives (Fig. 1) on normal and psoriasis-like keratinocyte differentiation were evaluated.…”
Section: Resultsmentioning
confidence: 99%
“…Potential drugs for this skin disease can be identified by measuring their effects on normal and psoriasis-like differentiation of submerged confluent keratinocyte cultures. In a 96-well format, upregulation of CK10 expression is used as a marker for normal differentiation (van Ruissen et al, 1996), whereas the expression and secretion of SKALP/Elafin is used as a marker for stressed epidermal differentiation (Alkemade et al, 1995;van Ruissen et al, 1996). An interesting drug candidate should dose-dependently suppress SKALP/Elafin expression, show little to no cytotoxicity at the pharmacological concentration range of 0.1-10 μM and preferably should not affect normal epidermal differentiation as determined by CK10 expression.…”
Section: Discussionmentioning
confidence: 99%
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