Induction of neonatal tolerance to GFP-labeled karyocytes in C57/B6 mice

Dovhyi, Roman; Pishel, Iryna; Butenko, G. M.; Skivka, Larysa

doi:10.1016/j.jim.2017.04.011

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…In addition, host neonatal mice may accept NPCs expressing exogenous proteins. In this study, neonatal mice exhibited tolerance to the EGFP antigen, which triggers a T-cell immune response, supporting the notion that their immune system is less mature compared to adults 33 , 44 .…”

Section: Discussionsupporting

confidence: 80%

Long-term viable chimeric nephrons generated from progenitor cells are a reliable model in cisplatin-induced toxicity

Matsui,

Yamanaka,

Chen

et al. 2023

Commun Biol

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Kidney organoids have shown promise as evaluation tools, but their in vitro maturity remains limited. Transplantation into adult mice has aided in maturation; however, their lack of urinary tract connection limits long-term viability. Thus, long-term viable generated nephrons have not been demonstrated. In this study, we present an approachable method in which mouse and rat renal progenitor cells are injected into the developing kidneys of neonatal mice, resulting in the generation of chimeric nephrons integrated with the host urinary tracts. These chimeric nephrons exhibit similar maturation to the host nephrons, long-term viability with excretion and reabsorption functions, and cisplatin-induced renal injury in both acute and chronic phases, as confirmed by single-cell RNA-sequencing. Additionally, induced human nephron progenitor cells differentiate into nephrons within the neonatal kidneys. Collectively, neonatal injection represents a promising approach for in vivo nephron generation, with potential applications in kidney regeneration, drug screening, and pathological analysis.

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Section: Discussionsupporting

confidence: 80%

Long-term viable chimeric nephrons generated from progenitor cells are a reliable model in cisplatin-induced toxicity

Matsui,

Yamanaka,

Chen

et al. 2023

Commun Biol

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“…For example, it has been proposed that rejection of GFP-labeled skin grafts is prevented in syngeneic mice by grafting GFP-positive donor splenocytes into recipient mice at the neonatal stage (Dovhyi, Pishel, Butenko, & Skivka, 2017). The present results indicate that immunological tolerance to functional Luc2 or EGFP can be induced by ubiquitous expression of their nonfunctional mutant proteins.…”

Section: Discussionmentioning

confidence: 52%

“…Several methods have been developed to avoid immune responses to xenogeneic gene products in mammals. For example, it has been proposed that rejection of GFP-labeled skin grafts is prevented in syngeneic mice by grafting GFP-positive donor splenocytes into recipient mice at the neonatal stage (Dovhyi, Pishel, Butenko, & Skivka, 2017). However, induction of the neonatal tolerance may be affected by various factors including antigens, mouse strains and the number of injected splenocytes that are often difficult to be controlled precisely.…”

Section: Discussionmentioning

confidence: 99%

Transgenic mice that accept Luciferase‐ or GFP‐expressing syngeneic tumor cells at high efficiencies

et al. 2018

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Jellyfish green fluorescent protein (GFP) and firefly luciferase can serve as versatile tracking markers for identification and quantification of transplanted cancer cells in vivo. However, immune reactions against these markers can hamper the formation of syngraft tumors and metastasis that follows. Here, we report two transgenic (Tg) mouse lines that express nonfunctional mutant marker proteins, namely modified firefly luciferase (Luc2) or enhanced GFP (EGFP). These mice, named as Tg-mLuc2 and Tg-mEGFP, turned out to be immunologically tolerant to the respective tracking markers and thus efficiently accepted syngeneic cancer cells expressing the active forms of the markers. We then injected intrarectally the F hybrid Tg mice (BALB/c × C57BL/6J) with Colon-26 (C26) colon cancer cells that originated from a BALB/c mouse. Even when C26 cells expressed active Luc2 or EGFP, they formed primary tumors in the Tg mice with only 10 cells per mouse compared with more than 10 cells required in the nontransgenic BALB/c hosts. Furthermore, we detected metastatic foci of C26 cells in the liver and lungs of the Tg mice by tracking the specific reporter activities. These results show the usefulness of the Tg mouse lines as recipients for transplantation experiments with the non-self tracking marker-expressing cells.

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Current state and development prospects of fundamental studies in research institutions of the National Academy of Medical Sciences of Ukraine

Цымбалюк¹,

Medvedovska²

2019

Journal NAMSU

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Fundamental scientific studies have always been and will always be a priority in Ukrainian academic medical science. Particular attention must be paid to the development of innovations and scientific and technical develop- ments in the medical industry. The National Academy of Medical Sciences of Ukraine endorses basic principles of further progressive development of medical science in the field of prevention of diseases, their early diagnosis, treatment and effective methods of rehabilitation. The Academy designates the strategic directions of research and innovation, ways to implement tasks and activities of a medical, economic, legal and social pattern, with the aim of maintaining and strengthening human health, as well as extending active longevity of the population. The implementation of the main directions of modern fundamental research at the level of the leading global institutions calls for a significant improvement in methodology, technologies and scien- tific workforce on par with the leading scientific institutions worldwide. This will give a new impetus to the development, implementation and continuity of fundamental research in medical science considering demographic, socio-hygienic and socio-economic factors that have a formative role for the health of the nation. The organization of research and production enterpris- es within the framework of academic institutions with the elimination of interdepartmental barriers, a more complex scientific research, the rational use of the funding, and the active implementation of results in health care practice is also of great importance to the further development of the funda- mental research of the medical science in Ukraine. The development of the global medical science is continuous, while priorities and key development points show the tendency to sway toward biomedical research and biotech- nology, genetic engineering and personalized high-tech medicine. Key words: fundamental studies, NAMS of Ukraine, medical science, innovation, health care.

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Induction of neonatal tolerance to GFP-labeled karyocytes in C57/B6 mice

Cited by 3 publications

References 17 publications

Long-term viable chimeric nephrons generated from progenitor cells are a reliable model in cisplatin-induced toxicity

Long-term viable chimeric nephrons generated from progenitor cells are a reliable model in cisplatin-induced toxicity

Transgenic mice that accept Luciferase‐ or GFP‐expressing syngeneic tumor cells at high efficiencies

Current state and development prospects of fundamental studies in research institutions of the National Academy of Medical Sciences of Ukraine

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