Induction of murine embryonic stem cell differentiation by medicinal plant extracts

Reynertson, Kurt A.; Charlson, Mary E.; Gudas, Lorraine J.

doi:10.1016/j.yexcr.2010.10.010

Cited by 21 publications

(19 citation statements)

References 52 publications

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“…In our study, addition of EA to cells resulted in decline in the activity of colonic ALP activity. Similar evidences supporting for EA inhibiting ALP activity have already been reported [33]. Administration of EA reduced the activity of LDH in HCT-15 cells.…”

Section: Discussionsupporting

confidence: 88%

Ellagic acid inhibits proliferation and induced apoptosis via the Akt signaling pathway in HCT-15 colon adenocarcinoma cells

2014

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Chemoprevention is regarded as one of the most promising and realistic approaches in the prevention of human cancer. Ellagic acid (EA) has been known for its chemopreventive activity against various cancers and numerous investigations have shown its apoptotic activity both in vivo and in vitro. The present study was focused to elucidate the anticancerous effect and the mode of action of EA against HCT-15 colon adenocarcinoma cells. Cell viability was assessed using trypan blue assay at different concentrations. EA also promoted cell cycle arrest substantially at G2/M phase in HCT-15 cells. The activities of alkaline phosphatase and lactate dehydrogenase were decreased upon EA treatment, which shows the antiproliferative and the cytotoxic effects, respectively. The production of reactive oxygen intermediates, which were examined by 2,7-dichlorodihydrofluorescein diacetate (H2DCF-DA), increased with time, after treatment with EA. In further studies, EA inhibited proliferation-associated markers proliferating cell nuclear antigen and cyclin D1. The induction of apoptosis was accompanied by a strong inactivation of phosphatidylinositol 3-kinase (PI3K)/Akt pathway by EA. The expression of PI3K and pAkt was down-regulated in EA-treated cells, compared to normal cells. Further, EA promoted the expression of Bax, caspase-3, and cytochrome c, and suppression of Bcl-2 activity in HCT-15 cells that was determined by western blot analysis. Increased annexin V apoptotic cells and DNA fragmentation also accompanied EA-induced apoptosis. In conclusion, EA increased the production of ROS, decreased cell proliferation, and induced apoptosis in HCT-15 cells, and thus can be used as an agent against colon cancer.

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Section: Discussionsupporting

confidence: 88%

Ellagic acid inhibits proliferation and induced apoptosis via the Akt signaling pathway in HCT-15 colon adenocarcinoma cells

2014

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“…Previous studies have reported from this plant triterpenes and flavonoid glycosides (Mitchell and Geissman, 1971). In addition, Reynertson et al (2011) isolated protocatechuic acid and the flavonoids rutin and 7-O-methylrutin with cytotoxic effect.…”

Section: Resultsmentioning

confidence: 99%

Screening of plants used in Mayan traditional medicine to treat cancer-like symptoms

Caamal-Fuentes

Torres-Tapia

Simá-Polanco

et al. 2011

Journal of Ethnopharmacology

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“…mRNA (5 μg) was reverse transcribed using the qScript cDNA SuperMix (Quanta Biosciences; 95048) (24). Semi-quantitative RT-PCR reactions were performed with 2 μg cDNA as template using a BioRad iCycler (25).…”

Section: Methodsmentioning

confidence: 99%

Role of NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex 4-Like 2 in Clear Cell Renal Cell Carcinoma

Minton

Mongan

et al. 2016

Clinical Cancer Research

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Purpose We delineated the functions of the HIF1α target NADH Dehydrogenase (Ubiquinone) 1 alpha subcomplex 4-like 2 (NDUFA4L2) in ccRCC and characterized NDUFA4L2 as a novel molecular target for ccRCC treatment. Experimental Design We evaluated normal kidney and ccRCC patient microarray and RNAseq data from Oncomine and The Cancer Genome Atlas (TCGA) for NDUFA4L2 mRNA levels and the clinical implications of high NDUFA4L2 expression. Additionally, we examined normal kidney and ccRCC patient tissue samples, human ccRCC cell lines, and murine models of ccRCC for NDUFA4L2 mRNA and protein expression. Utilizing shRNA, we performed NDUFA4L2 knockdown experiments and analyzed the proliferation, clonogenicity, metabolite levels, cell structure, and autophagy in ccRCC cell lines in culture. Results We found that NDUFA4L2 mRNA and protein are highly expressed in ccRCC samples but undetectable in normal kidney tissue samples, and that NDUFA4L2 mRNA expression correlates with tumor stage and lower overall survival. Additionally, we demonstrated that NDUFA4L2 is a HIF1α target in ccRCC and that NDUFA4L2 knockdown has a profound anti-proliferative effect, alters metabolic pathways, and causes major stress in cultured RCC cells. Conclusions Collectively, our data show that NDUFA4L2 is a novel molecular target for ccRCC treatment.

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Induction of murine embryonic stem cell differentiation by medicinal plant extracts

Cited by 21 publications

References 52 publications

Ellagic acid inhibits proliferation and induced apoptosis via the Akt signaling pathway in HCT-15 colon adenocarcinoma cells

Ellagic acid inhibits proliferation and induced apoptosis via the Akt signaling pathway in HCT-15 colon adenocarcinoma cells

Screening of plants used in Mayan traditional medicine to treat cancer-like symptoms

Role of NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex 4-Like 2 in Clear Cell Renal Cell Carcinoma

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