Induction of multiple shoots in Oryza sativa: roles of thidiazuron, 6-benzylaminopurine, decapitation, flooding, and Ethrel® treatments

Nautiyal, Aparna; Rashid, Abdur; Agnihotri, Ashish

doi:10.1007/s11627-022-10316-2

Cited by 5 publications

(2 citation statements)

References 103 publications

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“…These findings align with previous studies that extensively used cytokinins for in vitro shoot multiplication [13,14,16]. Furthermore, this study's findings on 'Shine Muscat' grapevines validate the beneficial effects of TDZ on main shoot development and multiple shoot formation, consistent with earlier research [43,44]. TDZ is well-known for its ability to stimulate shoot proliferation in various woody plants, as demonstrated in previous studies [45,46].…”

Section: Assessment Of the Impact Of Hormones On The Growth And Multi...supporting

confidence: 92%

Optimization of Plant Growth Regulators for In Vitro Mass Propagation of a Disease-Free ‘Shine Muscat’ Grapevine Cultivar

Kim,

Zebro,

Jang

et al. 2023

CIMB

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This study addresses the propagation challenges faced by ‘Shine Muscat’, a newly introduced premium grapevine cultivar in South Korea, where multiple viral infections pose considerable economic loss. The primary objective was to establish a robust in vitro propagation method for producing disease-free grapes and to identify effective plant growth regulators to facilitate large-scale mass cultivation. After experimentation, 2.0 µM 6-benzyladenine (BA) exhibited superior shoot formation in the Murashige and Skoog medium compared with kinetin and thidiazuron. Conversely, α-naphthaleneacetic acid (NAA) hindered shoot growth and induced callus formation, while indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA) demonstrated favorable root formation, with IBA showing better results overall. Furthermore, inter simple sequence repeat analysis confirmed the genetic stability of in vitro-cultivated seedlings using 2.0 μM BA and 1.0 μM IBA, validating the suitability of the developed propagation method for generating disease-free ‘Shine Muscat’ grapes. These findings offer promising prospects for commercial grape cultivation, ensuring a consistent supply of healthy grapes in the market.

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Section: Assessment Of the Impact Of Hormones On The Growth And Multi...supporting

confidence: 92%

Optimization of Plant Growth Regulators for In Vitro Mass Propagation of a Disease-Free ‘Shine Muscat’ Grapevine Cultivar

Kim,

Zebro,

Jang

et al. 2023

CIMB

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show abstract

“…vetiver grass [8], Cymbopogon schoenanthus [9], switchgrass (Panicum virgatum L.) [10], finger millet (Eleusine coracana (L.) Gaertn.) [11], Pennisetum purpureum [12], Pennisetum × advena 'Rubrum' [13], and cereals; as well as rice [14], wheat [15], wheat, durum wheat, oat, barley, triticale [16], and sorghum [17].…”

Section: Introductionmentioning

confidence: 99%

The Cytokinins BAP and 2-iP Modulate Different Molecular Mechanisms on Shoot Proliferation and Root Development in Lemongrass (Cymbopogon citratus)

Cárdenas-Aquino,

Camas-Reyes,

Valencia-Lozano

et al. 2023

Plants

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The known activities of cytokinins (CKs) are promoting shoot multiplication, root growth inhibition, and delaying senescence. 6-Benzylaminopurine (BAP) has been the most effective CK to induce shoot proliferation in cereal and grasses. Previously, we reported that in lemongrass (Cymbopogon citratus) micropropagation, BAP 10 µM induces high shoot proliferation, while the natural CK 6-(γ,γ-Dimethylallylamino)purine (2-iP) 10 µM shows less pronounced effects and developed rooting. To understand the molecular mechanisms involved, we perform a protein–protein interaction (PPI) network based on the genes of Brachypodium distachyon involved in shoot proliferation/repression, cell cycle, stem cell maintenance, auxin response factors, and CK signaling to analyze the molecular mechanisms in BAP versus 2-iP plants. A different pattern of gene expression was observed between BAP- versus 2-iP-treated plants. In shoots derived from BAP, we found upregulated genes that have already been demonstrated to be involved in de novo shoot proliferation development in several plant species; CK receptors (AHK3, ARR1), stem cell maintenance (STM, REV and CLV3), cell cycle regulation (CDKA-CYCD3 complex), as well as the auxin response factor (ARF5) and CK metabolism (CKX1). In contrast, in the 2-iP culture medium, there was an upregulation of genes involved in shoot repression (BRC1, MAX3), ARR4, a type A-response regulator (RR), and auxin metabolism (SHY2).

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Stress-induced somatic embryogenesis on seedlings of Azadirachta indica A. Juss. by thidiazuron and its inhibition by ethylene modulators

Nautiyal

Ramlal

Agnihotri

et al. 2023

Plant Cell Tiss Organ Cult

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Induction of multiple shoots in Oryza sativa: roles of thidiazuron, 6-benzylaminopurine, decapitation, flooding, and Ethrel® treatments

Cited by 5 publications

References 103 publications

Optimization of Plant Growth Regulators for In Vitro Mass Propagation of a Disease-Free ‘Shine Muscat’ Grapevine Cultivar

Optimization of Plant Growth Regulators for In Vitro Mass Propagation of a Disease-Free ‘Shine Muscat’ Grapevine Cultivar

The Cytokinins BAP and 2-iP Modulate Different Molecular Mechanisms on Shoot Proliferation and Root Development in Lemongrass (Cymbopogon citratus)

Stress-induced somatic embryogenesis on seedlings of Azadirachta indica A. Juss. by thidiazuron and its inhibition by ethylene modulators

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